Restriction map for
pF1KB8836
(Linear) (Six-base) MAPSORT of: KIBB8836.seq Check: 4970 from: 1 to: 1383
KIBB8836.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 735 1323 1383
Size: 315 420 588 60
Fragments arranged by size:
588 420 315 60
AflII C'TTAA_G
Cuts at: 0 352 1383
Size: 352 1031
ApoI r'AATT_y
Cuts at: 0 148 255 449 591 681 1011 1037 1205
Size: 148 107 194 142 90 330 26 168
Cuts at: 1205 1263 1383
Size: 58 120
Fragments arranged by size:
330 194 168 148 142 120 107 90
58 26
BanII G_rGCy'C
Cuts at: 0 343 847 1383
Size: 343 504 536
Fragments arranged by size:
536 504 343
BclI T'GATC_A
Cuts at: 0 1236 1383
Size: 1236 147
BfrBI ATG'CAT
Cuts at: 0 765 1383
Size: 765 618
BglII A'GATC_T
Cuts at: 0 299 1383
Size: 299 1084
BmrI ACTGGGnnnn_n'
Cuts at: 0 383 1383
Size: 383 1000
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 866 1383
Size: 866 517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 563 647 815 899 983 1067 1151
Size: 479 84 84 168 84 84 84 84
Cuts at: 1151 1383
Size: 232
Fragments arranged by size:
479 232 168 84 84 84 84 84
84
BsaBI GATnn'nnATC
Cuts at: 0 80 1383
Size: 80 1303
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 1383
Size: 339 1044
BsiHKAI G_wGCw'C
Cuts at: 0 343 847 1383
Size: 343 504 536
Fragments arranged by size:
536 504 343
BsmI GAATG_Cn'
Cuts at: 0 21 197 1168 1330 1383
Size: 21 176 971 162 53
Fragments arranged by size:
971 176 162 53 21
Bsp1286I G_dGCh'C
Cuts at: 0 343 847 1383
Size: 343 504 536
Fragments arranged by size:
536 504 343
BsrDI GCAATG_nn'
Cuts at: 0 1158 1383
Size: 1158 225
BsrGI T'GTAC_A
Cuts at: 0 438 774 1383
Size: 438 336 609
Fragments arranged by size:
609 438 336
BstYI r'GATC_y
Cuts at: 0 299 1383
Size: 299 1084
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1140 1175 1383
Size: 1140 35 208
Fragments arranged by size:
1140 208 35
EcoICRI GAG'CTC
Cuts at: 0 341 845 1383
Size: 341 504 538
Fragments arranged by size:
538 504 341
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 479 563 647 735 815 899 983
Size: 315 164 84 84 88 80 84 84
Cuts at: 983 1067 1151 1323 1383
Size: 84 84 172 60
Fragments arranged by size:
315 172 164 88 84 84 84 84
84 84 80 60
EcoO109I rG'GnC_Cy
Cuts at: 0 215 1383
Size: 215 1168
EcoRI G'AATT_C
Cuts at: 0 449 591 1011 1037 1205 1263 1383
Size: 449 142 420 26 168 58 120
Fragments arranged by size:
449 420 168 142 120 58 26
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 132 1383
Size: 132 1251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 498 530 678 710 1383
Size: 498 32 148 32 673
Fragments arranged by size:
673 498 148 32 32
HindIII A'AGCT_T
Cuts at: 0 662 998 1383
Size: 662 336 385
Fragments arranged by size:
662 385 336
MslI CAynn'nnrTG
Cuts at: 0 124 202 1214 1383
Size: 124 78 1012 169
Fragments arranged by size:
1012 169 124 78
NsiI A_TGCA'T
Cuts at: 0 767 1383
Size: 767 616
NspI r_CATG'y
Cuts at: 0 765 1383
Size: 765 618
PacI TTA_AT'TAA
Cuts at: 0 269 1383
Size: 269 1114
SacI G_AGCT'C
Cuts at: 0 343 847 1383
Size: 343 504 536
Fragments arranged by size:
536 504 343
SmlI C'TyrA_G
Cuts at: 0 352 1383
Size: 352 1031
SphI G_CATG'C
Cuts at: 0 765 1383
Size: 765 618
TatI w'GTAC_w
Cuts at: 0 438 774 928 1348 1383
Size: 438 336 154 420 35
Fragments arranged by size:
438 420 336 154 35
XmnI GAAnn'nnTTC
Cuts at: 0 412 617 1383
Size: 412 205 766
Fragments arranged by size:
766 412 205
Enzymes that do cut:
AcuI AflII ApoI BanII BclI BfrBI BglII BmrI
BplI BpmI BsaBI BsgI BsiHKAI BsmI Bsp1286I BsrDI
BsrGI BstYI CspCI EcoICRI Eco57MI EcoO109I EcoRI FalI
Hin4I HindIII MslI NsiI NspI PacI SacI SmlI
SphI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI
BmtI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoNI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI StyI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
ZraI