Restriction map for
pF1KB8835
(Linear) (Six-base) MAPSORT of: KIBB8835.seq Check: 9874 from: 1 to: 1377
KIBB8835.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AflIII A'CryG_T
Cuts at: 0 611 1377
Size: 611 766
AleI CACnn'nnGTG
Cuts at: 0 1161 1377
Size: 1161 216
AlwNI CAG_nnn'CTG
Cuts at: 0 186 1377
Size: 186 1191
ApaLI G'TGCA_C
Cuts at: 0 230 1002 1377
Size: 230 772 375
Fragments arranged by size:
772 375 230
ApoI r'AATT_y
Cuts at: 0 280 1377
Size: 280 1097
AvaI C'yCGr_G
Cuts at: 0 873 911 1227 1377
Size: 873 38 316 150
Fragments arranged by size:
873 316 150 38
BanI G'GyrC_C
Cuts at: 0 271 1295 1377
Size: 271 1024 82
Fragments arranged by size:
1024 271 82
BanII G_rGCy'C
Cuts at: 0 386 1377
Size: 386 991
BbeI G_GCGC'C
Cuts at: 0 275 1377
Size: 275 1102
BbsI GAAGACnn'nnnn_
Cuts at: 0 569 1038 1377
Size: 569 469 339
Fragments arranged by size:
569 469 339
BbvCI CC'TCA_GC
Cuts at: 0 367 1377
Size: 367 1010
BglI GCCn_nnn'nGGC
Cuts at: 0 916 1351 1377
Size: 916 435 26
Fragments arranged by size:
916 435 26
Bme1580I G_kGCm'C
Cuts at: 0 234 1006 1015 1084 1377
Size: 234 772 9 69 293
Fragments arranged by size:
772 293 234 69 9
BmgBI CAC'GTC
Cuts at: 0 102 612 1263 1377
Size: 102 510 651 114
Fragments arranged by size:
651 510 114 102
BmtI G_CTAG'C
Cuts at: 0 425 1377
Size: 425 952
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 565 670 698 1360 1377
Size: 449 116 105 28 662 17
Fragments arranged by size:
662 449 116 105 28 17
Bpu10I CC'TnA_GC
Cuts at: 0 367 1377
Size: 367 1010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1285 1377
Size: 109 1176 92
Fragments arranged by size:
1176 109 92
BsaI GGTCTCn'nnnn_
Cuts at: 0 219 1377
Size: 219 1158
BsaAI yAC'GTr
Cuts at: 0 342 1377
Size: 342 1035
BsaHI Gr'CG_yC
Cuts at: 0 272 1377
Size: 272 1105
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 698 728 1234 1264 1377
Size: 698 30 506 30 113
Fragments arranged by size:
698 506 113 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 244 448 500 1377
Size: 100 144 204 52 877
Fragments arranged by size:
877 204 144 100 52
BseYI C'CCAG_C
Cuts at: 0 196 757 1377
Size: 196 561 620
Fragments arranged by size:
620 561 196
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1207 1377
Size: 1207 170
BsiEI CG_ry'CG
Cuts at: 0 1328 1377
Size: 1328 49
BsiHKAI G_wGCw'C
Cuts at: 0 234 341 1006 1377
Size: 234 107 665 371
Fragments arranged by size:
665 371 234 107
BsmI GAATG_Cn'
Cuts at: 0 366 1377
Size: 366 1011
Bsp1286I G_dGCh'C
Cuts at: 0 234 341 386 1006 1015 1084 1377
Size: 234 107 45 620 9 69 293
Fragments arranged by size:
620 293 234 107 69 45 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 201 335 1229 1377
Size: 201 134 894 148
Fragments arranged by size:
894 201 148 134
BsrBI CCG'CTC
Cuts at: 0 90 1377
Size: 90 1287
BsrFI r'CCGG_y
Cuts at: 0 662 1377
Size: 662 715
BssSI C'ACGA_G
Cuts at: 0 43 1161 1377
Size: 43 1118 216
Fragments arranged by size:
1118 216 43
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1010 1377
Size: 1010 367
BtgI C'CryG_G
Cuts at: 0 713 902 1322 1377
Size: 713 189 420 55
Fragments arranged by size:
713 420 189 55
DraIII CAC_nnn'GTG
Cuts at: 0 345 1010 1377
Size: 345 665 367
Fragments arranged by size:
665 367 345
EaeI y'GGCC_r
Cuts at: 0 39 113 152 242 532 720 899 1325
Size: 39 74 39 90 290 188 179 426
Cuts at: 1325 1352 1377
Size: 27 25
Fragments arranged by size:
426 290 188 179 90 74 39 39
27 25
EagI C'GGCC_G
Cuts at: 0 1325 1377
Size: 1325 52
EarI CTCTTCn'nnn_
Cuts at: 0 581 1377
Size: 581 796
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 565 670 698 1360 1377
Size: 449 116 105 28 662 17
Fragments arranged by size:
662 449 116 105 28 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 400 1377
Size: 400 977
EcoO109I rG'GnC_Cy
Cuts at: 0 473 828 952 1377
Size: 473 355 124 425
Fragments arranged by size:
473 425 355 124
EcoRI G'AATT_C
Cuts at: 0 280 1377
Size: 280 1097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 165 1062 1377
Size: 165 897 315
Fragments arranged by size:
897 315 165
HaeII r_GCGC'y
Cuts at: 0 275 446 1377
Size: 275 171 931
Fragments arranged by size:
931 275 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 598 630 1377
Size: 598 32 747
Fragments arranged by size:
747 598 32
HincII GTy'rAC
Cuts at: 0 303 1377
Size: 303 1074
KasI G'GCGC_C
Cuts at: 0 271 1377
Size: 271 1106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 788 1377
Size: 788 589
MscI TGG'CCA
Cuts at: 0 722 901 1377
Size: 722 179 476
Fragments arranged by size:
722 476 179
MslI CAynn'nnrTG
Cuts at: 0 1075 1135 1145 1161 1377
Size: 1075 60 10 16 216
Fragments arranged by size:
1075 216 60 16 10
MspA1I CmG'CkG
Cuts at: 0 33 895 1247 1377
Size: 33 862 352 130
Fragments arranged by size:
862 352 130 33
NarI GG'CG_CC
Cuts at: 0 272 1377
Size: 272 1105
NcoI C'CATG_G
Cuts at: 0 902 1377
Size: 902 475
NheI G'CTAG_C
Cuts at: 0 421 1377
Size: 421 956
NspI r_CATG'y
Cuts at: 0 1056 1377
Size: 1056 321
PflMI CCAn_nnn'nTGG
Cuts at: 0 1032 1377
Size: 1032 345
PfoI T'CCnGG_A
Cuts at: 0 843 1377
Size: 843 534
PmlI CAC'GTG
Cuts at: 0 342 1377
Size: 342 1035
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 184 216 1377
Size: 76 32 76 32 1161
Fragments arranged by size:
1161 76 76 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 473 952 1377
Size: 473 479 425
Fragments arranged by size:
479 473 425
PstI C_TGCA'G
Cuts at: 0 212 332 1377
Size: 212 120 1045
Fragments arranged by size:
1045 212 120
PvuII CAG'CTG
Cuts at: 0 33 1377
Size: 33 1344
SanDI GG'GwC_CC
Cuts at: 0 952 1377
Size: 952 425
SfcI C'TryA_G
Cuts at: 0 208 328 764 798 1377
Size: 208 120 436 34 579
Fragments arranged by size:
579 436 208 120 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1351 1377
Size: 1351 26
SfoI GGC'GCC
Cuts at: 0 273 1377
Size: 273 1104
SmaI CCC'GGG
Cuts at: 0 913 1377
Size: 913 464
SmlI C'TyrA_G
Cuts at: 0 124 1300 1377
Size: 124 1176 77
Fragments arranged by size:
1176 124 77
StuI AGG'CCT
Cuts at: 0 395 683 793 1377
Size: 395 288 110 584
Fragments arranged by size:
584 395 288 110
StyI C'CwwG_G
Cuts at: 0 275 726 902 1377
Size: 275 451 176 475
Fragments arranged by size:
475 451 275 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 706 1377
Size: 706 671
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 698 730 1377
Size: 698 32 647
Fragments arranged by size:
698 647 32
Tth111I GACn'n_nGTC
Cuts at: 0 298 1377
Size: 298 1079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 876 1377
Size: 876 501
XmaI C'CCGG_G
Cuts at: 0 911 1377
Size: 911 466
Enzymes that do cut:
AflIII AleI AlwNI ApaLI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BglI Bme1580I BmgBI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI
BssSI BstAPI BtgI DraIII EaeI EagI EarI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NarI NcoI NheI NspI
PflMI PfoI PmlI PpiI PpuMI PstI PvuII SanDI
SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII
BlpI BmrI BplI BsaBI BsaWI BsiWI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI
EciI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI NaeI NdeI NgoMIV NotI NruI
NsiI PacI PasI PciI PmeI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TatI XbaI XhoI XmnI ZraI