Restriction map for
pF1KB8833
(Linear) (Six-base) MAPSORT of: KIBB8833.seq Check: 7760 from: 1 to: 1359
KIBB8833.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AccI GT'mk_AC
Cuts at: 0 71 130 1359
Size: 71 59 1229
Fragments arranged by size:
1229 71 59
Acc65I G'GTAC_C
Cuts at: 0 388 1359
Size: 388 971
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 364 1359
Size: 193 171 995
Fragments arranged by size:
995 193 171
AhdI GACnn_n'nnGTC
Cuts at: 0 126 1359
Size: 126 1233
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 639 671 1359
Size: 639 32 688
Fragments arranged by size:
688 639 32
AlwNI CAG_nnn'CTG
Cuts at: 0 196 1359
Size: 196 1163
ApoI r'AATT_y
Cuts at: 0 1231 1359
Size: 1231 128
AvaI C'yCGr_G
Cuts at: 0 11 250 1359
Size: 11 239 1109
Fragments arranged by size:
1109 239 11
BamHI G'GATC_C
Cuts at: 0 289 1359
Size: 289 1070
BanI G'GyrC_C
Cuts at: 0 55 388 1359
Size: 55 333 971
Fragments arranged by size:
971 333 55
BanII G_rGCy'C
Cuts at: 0 540 1214 1359
Size: 540 674 145
Fragments arranged by size:
674 540 145
BbsI GAAGACnn'nnnn_
Cuts at: 0 125 1359
Size: 125 1234
BglI GCCn_nnn'nGGC
Cuts at: 0 437 1359
Size: 437 922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 661 1292 1359
Size: 524 137 631 67
Fragments arranged by size:
631 524 137 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 840 1359
Size: 840 519
BsaAI yAC'GTr
Cuts at: 0 730 1359
Size: 730 629
BsaWI w'CCGG_w
Cuts at: 0 560 1359
Size: 560 799
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 522 552 1265 1295 1359
Size: 522 30 713 30 64
Fragments arranged by size:
713 522 64 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 1359
Size: 139 1220
BseYI C'CCAG_C
Cuts at: 0 184 919 1359
Size: 184 735 440
Fragments arranged by size:
735 440 184
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 1359
Size: 931 428
BsiHKAI G_wGCw'C
Cuts at: 0 532 612 1359
Size: 532 80 747
Fragments arranged by size:
747 532 80
BsmI GAATG_Cn'
Cuts at: 0 726 1359
Size: 726 633
BsmBI CGTCTCn'nnnn_
Cuts at: 0 86 1359
Size: 86 1273
Bsp1286I G_dGCh'C
Cuts at: 0 532 540 612 1214 1359
Size: 532 8 72 602 145
Fragments arranged by size:
602 532 145 72 8
BspEI T'CCGG_A
Cuts at: 0 560 1359
Size: 560 799
BstYI r'GATC_y
Cuts at: 0 289 472 1359
Size: 289 183 887
Fragments arranged by size:
887 289 183
BstZ17I GTA'TAC
Cuts at: 0 72 1359
Size: 72 1287
Bsu36I CC'TnA_GG
Cuts at: 0 233 1359
Size: 233 1126
BtgI C'CryG_G
Cuts at: 0 884 1359
Size: 884 475
DrdI GACnn_nn'nnGTC
Cuts at: 0 886 1359
Size: 886 473
EaeI y'GGCC_r
Cuts at: 0 29 1359
Size: 29 1330
EarI CTCTTCn'nnn_
Cuts at: 0 84 1359
Size: 84 1275
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 364 524 661 1292 1359
Size: 193 171 160 137 631 67
Fragments arranged by size:
631 193 171 160 137 67
EcoNI CCTnn'n_nnAGG
Cuts at: 0 622 1359
Size: 622 737
EcoO109I rG'GnC_Cy
Cuts at: 0 327 407 627 1359
Size: 327 80 220 732
Fragments arranged by size:
732 327 220 80
EcoRI G'AATT_C
Cuts at: 0 1231 1359
Size: 1231 128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 594 1359
Size: 594 765
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 1076 1108 1359
Size: 112 32 932 32 251
Fragments arranged by size:
932 251 112 32 32
HincII GTy'rAC
Cuts at: 0 935 1359
Size: 935 424
HpaI GTT'AAC
Cuts at: 0 935 1359
Size: 935 424
KpnI G_GTAC'C
Cuts at: 0 392 1359
Size: 392 967
MslI CAynn'nnrTG
Cuts at: 0 946 1359
Size: 946 413
MspA1I CmG'CkG
Cuts at: 0 34 299 907 1359
Size: 34 265 608 452
Fragments arranged by size:
608 452 265 34
NcoI C'CATG_G
Cuts at: 0 884 1359
Size: 884 475
PfoI T'CCnGG_A
Cuts at: 0 638 1222 1359
Size: 638 584 137
Fragments arranged by size:
638 584 137
PpuMI rG'GwC_Cy
Cuts at: 0 407 627 1359
Size: 407 220 732
Fragments arranged by size:
732 407 220
PsiI TTA'TAA
Cuts at: 0 587 1054 1359
Size: 587 467 305
Fragments arranged by size:
587 467 305
PspXI vC'TCGA_Gb
Cuts at: 0 250 1359
Size: 250 1109
PvuII CAG'CTG
Cuts at: 0 907 1359
Size: 907 452
SmlI C'TyrA_G
Cuts at: 0 250 819 1359
Size: 250 569 540
Fragments arranged by size:
569 540 250
StyI C'CwwG_G
Cuts at: 0 317 323 506 677 884 1359
Size: 317 6 183 171 207 475
Fragments arranged by size:
475 317 207 183 171 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 727 1359
Size: 727 632
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 522 554 1359
Size: 522 32 805
Fragments arranged by size:
805 522 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 301 548 732 1359
Size: 301 247 184 627
Fragments arranged by size:
627 301 247 184
XhoI C'TCGA_G
Cuts at: 0 250 1359
Size: 250 1109
XmnI GAAnn'nnTTC
Cuts at: 0 1231 1359
Size: 1231 128
Enzymes that do cut:
AccI Acc65I AcuI AhdI AloI AlwNI ApoI AvaI
BamHI BanI BanII BbsI BglI BpmI BpuEI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BstYI BstZ17I Bsu36I BtgI DrdI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII
HpaI KpnI MslI MspA1I NcoI PfoI PpuMI PsiI
PspXI PvuII SmlI StyI TaqII TstI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AleI
AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI BsaBI
BsaHI BsiEI BsiWI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BtgZI
BtsI ClaI CspCI DraI DraIII EagI EciI EcoICRI
EcoRV FseI FspI FspAI HaeII HindIII KasI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PspOMI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XmaI ZraI