Restriction map for
pF1KB8832
(Linear) (Six-base) MAPSORT of: KIBB8832.seq Check: 1847 from: 1 to: 1356
KIBB8832.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 659 1356
Size: 659 697
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 774 942 1110 1194 1356
Size: 774 168 168 84 162
Fragments arranged by size:
774 168 168 162 84
AfeI AGC'GCT
Cuts at: 0 148 883 1356
Size: 148 735 473
Fragments arranged by size:
735 473 148
AflIII A'CryG_T
Cuts at: 0 554 1356
Size: 554 802
AhdI GACnn_n'nnGTC
Cuts at: 0 580 1356
Size: 580 776
AleI CACnn'nnGTG
Cuts at: 0 110 599 724 849 1048 1356
Size: 110 489 125 125 199 308
Fragments arranged by size:
489 308 199 125 125 110
AlwNI CAG_nnn'CTG
Cuts at: 0 366 1356
Size: 366 990
ApaI G_GGCC'C
Cuts at: 0 1248 1356
Size: 1248 108
ApaLI G'TGCA_C
Cuts at: 0 152 898 1356
Size: 152 746 458
Fragments arranged by size:
746 458 152
AvaI C'yCGr_G
Cuts at: 0 663 940 1108 1356
Size: 663 277 168 248
Fragments arranged by size:
663 277 248 168
BanI G'GyrC_C
Cuts at: 0 353 523 1356
Size: 353 170 833
Fragments arranged by size:
833 353 170
BanII G_rGCy'C
Cuts at: 0 105 568 634 1248 1356
Size: 105 463 66 614 108
Fragments arranged by size:
614 463 108 105 66
BbeI G_GCGC'C
Cuts at: 0 357 527 1356
Size: 357 170 829
Fragments arranged by size:
829 357 170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 855 889 1287 1321 1356
Size: 855 34 398 34 35
Fragments arranged by size:
855 398 35 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 245 375 833 1356
Size: 245 130 458 523
Fragments arranged by size:
523 458 245 130
Bme1580I G_kGCm'C
Cuts at: 0 156 505 688 902 940 1248 1300 1356
Size: 156 349 183 214 38 308 52 56
Fragments arranged by size:
349 308 214 183 156 56 52 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 1356
Size: 730 626
BsaAI yAC'GTr
Cuts at: 0 555 1046 1356
Size: 555 491 310
Fragments arranged by size:
555 491 310
BsaHI Gr'CG_yC
Cuts at: 0 354 524 590 1356
Size: 354 170 66 766
Fragments arranged by size:
766 354 170 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 1134 1135 1356
Size: 66 1068 1 221
Fragments arranged by size:
1068 221 66 1
BsiEI CG_ry'CG
Cuts at: 0 120 249 315 951 966 1356
Size: 120 129 66 636 15 390
Fragments arranged by size:
636 390 129 120 66 15
BsiHKAI G_wGCw'C
Cuts at: 0 105 156 634 902 1356
Size: 105 51 478 268 454
Fragments arranged by size:
478 454 268 105 51
BsiWI C'GTAC_G
Cuts at: 0 761 1356
Size: 761 595
BsmI GAATG_Cn'
Cuts at: 0 142 1356
Size: 142 1214
Bsp1286I G_dGCh'C
Cuts at: 0 105 156 505 568 634 688 902 940
Size: 105 51 349 63 66 54 214 38
Cuts at: 940 1248 1300 1356
Size: 308 52 56
Fragments arranged by size:
349 308 214 105 66 63 56 54
52 51 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 659 1356
Size: 659 697
BsrBI CCG'CTC
Cuts at: 0 375 840 968 1223 1356
Size: 375 465 128 255 133
Fragments arranged by size:
465 375 255 133 128
BsrFI r'CCGG_y
Cuts at: 0 275 314 350 986 1084 1320 1356
Size: 275 39 36 636 98 236 36
Fragments arranged by size:
636 275 236 98 39 36 36
BssHII G'CGCG_C
Cuts at: 0 419 494 544 1254 1356
Size: 419 75 50 710 102
Fragments arranged by size:
710 419 102 75 50
BssSI C'ACGA_G
Cuts at: 0 42 1356
Size: 42 1314
BstAPI GCAn_nnn'nTGC
Cuts at: 0 160 221 1356
Size: 160 61 1135
Fragments arranged by size:
1135 160 61
BstXI CCAn_nnnn'nTGG
Cuts at: 0 601 1356
Size: 601 755
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 297 1356
Size: 297 1059
BtsI GCAGTG_nn'
Cuts at: 0 26 222 505 1147 1298 1356
Size: 26 196 283 642 151 58
Fragments arranged by size:
642 283 196 151 58 26
EaeI y'GGCC_r
Cuts at: 0 117 195 246 312 841 948 1086 1177
Size: 117 78 51 66 529 107 138 91
Cuts at: 1177 1356
Size: 179
Fragments arranged by size:
529 179 138 117 107 91 78 66
51
EagI C'GGCC_G
Cuts at: 0 117 246 312 948 1356
Size: 117 129 66 636 408
Fragments arranged by size:
636 408 129 117 66
EarI CTCTTCn'nnn_
Cuts at: 0 433 1356
Size: 433 923
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 226 1356
Size: 226 1130
EcoICRI GAG'CTC
Cuts at: 0 103 632 1356
Size: 103 529 724
Fragments arranged by size:
724 529 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 774 942 1110 1194 1356
Size: 730 44 168 168 84 162
Fragments arranged by size:
730 168 168 162 84 44
FspI TGC'GCA
Cuts at: 0 1191 1356
Size: 1191 165
HaeII r_GCGC'y
Cuts at: 0 150 321 340 357 527 719 885 1356
Size: 150 171 19 17 170 192 166 471
Fragments arranged by size:
471 192 171 170 166 150 19 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 566 598 1356
Size: 566 32 758
Fragments arranged by size:
758 566 32
HindIII A'AGCT_T
Cuts at: 0 231 1141 1356
Size: 231 910 215
Fragments arranged by size:
910 231 215
KasI G'GCGC_C
Cuts at: 0 353 523 1356
Size: 353 170 833
Fragments arranged by size:
833 353 170
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 1356
Size: 911 445
MscI TGG'CCA
Cuts at: 0 197 1356
Size: 197 1159
MslI CAynn'nnrTG
Cuts at: 0 110 553 599 724 849 1048 1101 1356
Size: 110 443 46 125 125 199 53 255
Fragments arranged by size:
443 255 199 125 125 110 53 46
MspA1I CmG'CkG
Cuts at: 0 28 245 386 393 491 1019 1183 1242
Size: 28 217 141 7 98 528 164 59
Cuts at: 1242 1267 1356
Size: 25 89
Fragments arranged by size:
528 217 164 141 98 89 59 28
25 7
NaeI GCC'GGC
Cuts at: 0 316 1086 1322 1356
Size: 316 770 236 34
Fragments arranged by size:
770 316 236 34
NarI GG'CG_CC
Cuts at: 0 354 524 1356
Size: 354 170 832
Fragments arranged by size:
832 354 170
NgoMIV G'CCGG_C
Cuts at: 0 314 1084 1320 1356
Size: 314 770 236 36
Fragments arranged by size:
770 314 236 36
NotI GC'GGCC_GC
Cuts at: 0 948 1356
Size: 948 408
NspI r_CATG'y
Cuts at: 0 193 311 770 850 1356
Size: 193 118 459 80 506
Fragments arranged by size:
506 459 193 118 80
PfoI T'CCnGG_A
Cuts at: 0 559 707 1356
Size: 559 148 649
Fragments arranged by size:
649 559 148
PmlI CAC'GTG
Cuts at: 0 555 1046 1356
Size: 555 491 310
Fragments arranged by size:
555 491 310
PspOMI G'GGCC_C
Cuts at: 0 1244 1356
Size: 1244 112
PstI C_TGCA'G
Cuts at: 0 217 656 1356
Size: 217 439 700
Fragments arranged by size:
700 439 217
SacI G_AGCT'C
Cuts at: 0 105 634 1356
Size: 105 529 722
Fragments arranged by size:
722 529 105
SapI GCTCTTCn'nnn_
Cuts at: 0 433 1356
Size: 433 923
SexAI A'CCwGG_T
Cuts at: 0 890 1356
Size: 890 466
SfcI C'TryA_G
Cuts at: 0 72 213 652 1356
Size: 72 141 439 704
Fragments arranged by size:
704 439 141 72
SfoI GGC'GCC
Cuts at: 0 355 525 1356
Size: 355 170 831
Fragments arranged by size:
831 355 170
SgrAI Cr'CCGG_yG
Cuts at: 0 350 986 1356
Size: 350 636 370
Fragments arranged by size:
636 370 350
SphI G_CATG'C
Cuts at: 0 193 1356
Size: 193 1163
StuI AGG'CCT
Cuts at: 0 1123 1356
Size: 1123 233
StyI C'CwwG_G
Cuts at: 0 452 1356
Size: 452 904
XmnI GAAnn'nnTTC
Cuts at: 0 676 928 1356
Size: 676 252 428
Fragments arranged by size:
676 428 252
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BcgI BglI Bme1580I
BpmI BsaAI BsaHI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI
BtgZI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI
FspI HaeII Hin4I HindIII KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NotI NspI PfoI PmlI
PspOMI PstI SacI SapI SexAI SfcI SfoI SgrAI
SphI StuI StyI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BseYI BsmBI BspEI BspHI BsrDI BsrGI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI ClaI CspCI DraI
DraIII DrdI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspAI HincII HpaI KpnI MfeI MluI NcoI NdeI
NheI NruI NsiI PacI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SfiI SgfI
SmaI SmlI SnaBI SpeI SrfI SspI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI