(Linear) (Six-base) MAPSORT of: KIBB8830.seq  Check: 3614  from: 1  to: 1320



KIBB8830.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

Acc65I G'GTAC_C

Cuts at:      0     916    1320 
   Size:        916     404
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     323     820    1276    1320 
   Size:        323     497     456      44

  Fragments arranged by size: 

                 497     456     323      44
 
AlwNI CAG_nnn'CTG

Cuts at:      0     145     282    1320 
   Size:        145     137    1038

  Fragments arranged by size: 

                1038     145     137
 
ApoI r'AATT_y

Cuts at:      0     362     462     698    1320 
   Size:        362     100     236     622

  Fragments arranged by size: 

                 622     362     236     100
 
BanI G'GyrC_C

Cuts at:      0     916    1320 
   Size:        916     404
 
BanII G_rGCy'C

Cuts at:      0    1079    1320 
   Size:       1079     241
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     953    1044    1320 
   Size:        953      91     276

  Fragments arranged by size: 

                 953     276      91
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     870    1082    1320 
   Size:        870     212     238

  Fragments arranged by size: 

                 870     238     212
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      51      81     815     845    1320 
   Size:         51      30     734      30     475

  Fragments arranged by size: 

                 734     475      51      30      30
 
Bsp1286I G_dGCh'C

Cuts at:      0    1079    1320 
   Size:       1079     241
 
BsrGI T'GTAC_A

Cuts at:      0    1198    1320 
   Size:       1198     122
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     225     860    1320 
   Size:        225     635     460

  Fragments arranged by size: 

                 635     460     225
 
BtgI C'CryG_G

Cuts at:      0     293    1320 
   Size:        293    1027
 
BtsI GCAGTG_nn'

Cuts at:      0     307     449    1320 
   Size:        307     142     871

  Fragments arranged by size: 

                 871     307     142
 
ClaI AT'CG_AT

Cuts at:      0     252    1217    1320 
   Size:        252     965     103

  Fragments arranged by size: 

                 965     252     103
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     980    1048    1320 
   Size:        980      68     272

  Fragments arranged by size: 

                 980     272      68
 
EarI CTCTTCn'nnn_

Cuts at:      0     342     995    1320 
   Size:        342     653     325

  Fragments arranged by size: 

                 653     342     325
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     323     820    1276    1320 
   Size:        323     497     456      44

  Fragments arranged by size: 

                 497     456     323      44
 
EcoO109I rG'GnC_Cy

Cuts at:      0     622    1095    1320 
   Size:        622     473     225

  Fragments arranged by size: 

                 622     473     225
 
HaeII r_GCGC'y

Cuts at:      0    1106    1320 
   Size:       1106     214
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     504     536    1320 
   Size:        504      32     784

  Fragments arranged by size: 

                 784     504      32
 
KpnI G_GTAC'C

Cuts at:      0     920    1320 
   Size:        920     400
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     206     752    1320 
   Size:         37     169     546     568

  Fragments arranged by size: 

                 568     546     169      37
 
MslI CAynn'nnrTG

Cuts at:      0     242     371     572    1320 
   Size:        242     129     201     748

  Fragments arranged by size: 

                 748     242     201     129
 
MspA1I CmG'CkG

Cuts at:      0     209    1320 
   Size:        209    1111
 
PpuMI rG'GwC_Cy

Cuts at:      0     622    1095    1320 
   Size:        622     473     225

  Fragments arranged by size: 

                 622     473     225
 
PsiI TTA'TAA

Cuts at:      0    1091    1271    1320 
   Size:       1091     180      49

  Fragments arranged by size: 

                1091     180      49
 
PstI C_TGCA'G

Cuts at:      0     770    1169    1320 
   Size:        770     399     151

  Fragments arranged by size: 

                 770     399     151
 
PvuII CAG'CTG

Cuts at:      0     209    1320 
   Size:        209    1111
 
ScaI AGT'ACT

Cuts at:      0    1289    1320 
   Size:       1289      31
 
SexAI A'CCwGG_T

Cuts at:      0     647    1320 
   Size:        647     673
 
SfcI C'TryA_G

Cuts at:      0     766     978    1165    1320 
   Size:        766     212     187     155

  Fragments arranged by size: 

                 766     212     187     155
 
SpeI A'CTAG_T

Cuts at:      0      52    1320 
   Size:         52    1268
 
SspI AAT'ATT

Cuts at:      0      21    1320 
   Size:         21    1299
 
TatI w'GTAC_w

Cuts at:      0    1198    1287    1320 
   Size:       1198      89      33

  Fragments arranged by size: 

                1198      89      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      51      83    1320 
   Size:         51      32    1237

  Fragments arranged by size: 

                1237      51      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     747    1320 
   Size:        747     573
 
XmnI GAAnn'nnTTC

Cuts at:      0    1124    1320 
   Size:       1124     196
 

 Enzymes that do cut:

   Acc65I     AcuI    AlwNI     ApoI     BanI    BanII     BbsI     BmrI
    BsaXI Bsp1286I    BsrGI    BstXI     BtgI     BtsI     ClaI     DrdI
     EarI  Eco57MI EcoO109I    HaeII    Hin4I     KpnI     MmeI     MslI
   MspA1I    PpuMI     PsiI     PstI    PvuII     ScaI    SexAI     SfcI
     SpeI     SspI     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
    BtgZI    CspCI     DraI   DraIII     EaeI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SphI     SrfI     StuI     StyI     SwaI
    TaqII  Tth111I     XbaI     XhoI     XmaI     ZraI