Restriction map for
pF1KB8829
(Linear) (Six-base) MAPSORT of: KIBB8829.seq Check: 3978 from: 1 to: 1311
KIBB8829.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 486 990 1311
Size: 486 504 321
Fragments arranged by size:
504 486 321
AflIII A'CryG_T
Cuts at: 0 121 1311
Size: 121 1190
AleI CACnn'nnGTG
Cuts at: 0 195 1049 1311
Size: 195 854 262
Fragments arranged by size:
854 262 195
AlwNI CAG_nnn'CTG
Cuts at: 0 1274 1311
Size: 1274 37
ApoI r'AATT_y
Cuts at: 0 205 1208 1311
Size: 205 1003 103
Fragments arranged by size:
1003 205 103
AseI AT'TA_AT
Cuts at: 0 1304 1311
Size: 1304 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 74 783 1311
Size: 74 709 528
Fragments arranged by size:
709 528 74
BglII A'GATC_T
Cuts at: 0 1009 1311
Size: 1009 302
BmrI ACTGGGnnnn_n'
Cuts at: 0 554 674 842 1311
Size: 554 120 168 469
Fragments arranged by size:
554 469 168 120
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 28 1311
Size: 28 1283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 482 818 902 986 1154 1238 1311
Size: 116 366 336 84 84 168 84 73
Fragments arranged by size:
366 336 168 116 84 84 84 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 1311
Size: 270 1041
BsaBI GATnn'nnATC
Cuts at: 0 147 1311
Size: 147 1164
BsaWI w'CCGG_w
Cuts at: 0 63 1311
Size: 63 1248
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 1311
Size: 44 1267
BsmI GAATG_Cn'
Cuts at: 0 350 794 1311
Size: 350 444 517
Fragments arranged by size:
517 444 350
BspEI T'CCGG_A
Cuts at: 0 63 1311
Size: 63 1248
BspHI T'CATG_A
Cuts at: 0 948 1311
Size: 948 363
BsrDI GCAATG_nn'
Cuts at: 0 913 997 1081 1311
Size: 913 84 84 230
Fragments arranged by size:
913 230 84 84
BstBI TT'CG_AA
Cuts at: 0 270 354 522 774 1026 1311
Size: 270 84 168 252 252 285
Fragments arranged by size:
285 270 252 252 168 84
BstYI r'GATC_y
Cuts at: 0 1009 1311
Size: 1009 302
BtsI GCAGTG_nn'
Cuts at: 0 261 1311
Size: 261 1050
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 891 926 1311
Size: 891 35 385
Fragments arranged by size:
891 385 35
EarI CTCTTCn'nnn_
Cuts at: 0 95 1311
Size: 95 1216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 482 486 818 902 986 990 1154
Size: 116 366 4 332 84 84 4 164
Cuts at: 1154 1238 1311
Size: 84 73
Fragments arranged by size:
366 332 164 116 84 84 84 73
4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 156 1311
Size: 156 1155
EcoRI G'AATT_C
Cuts at: 0 1208 1311
Size: 1208 103
HindIII A'AGCT_T
Cuts at: 0 768 1311
Size: 768 543
MfeI C'AATT_G
Cuts at: 0 672 1311
Size: 672 639
MslI CAynn'nnrTG
Cuts at: 0 195 561 695 787 1049 1115 1311
Size: 195 366 134 92 262 66 196
Fragments arranged by size:
366 262 196 195 134 92 66
NdeI CA'TA_TG
Cuts at: 0 558 1311
Size: 558 753
NspI r_CATG'y
Cuts at: 0 75 125 1311
Size: 75 50 1186
Fragments arranged by size:
1186 75 50
PciI A'CATG_T
Cuts at: 0 121 1311
Size: 121 1190
PfoI T'CCnGG_A
Cuts at: 0 93 1311
Size: 93 1218
PsiI TTA'TAA
Cuts at: 0 392 766 1311
Size: 392 374 545
Fragments arranged by size:
545 392 374
SmlI C'TyrA_G
Cuts at: 0 249 1311
Size: 249 1062
StuI AGG'CCT
Cuts at: 0 285 807 1311
Size: 285 522 504
Fragments arranged by size:
522 504 285
StyI C'CwwG_G
Cuts at: 0 416 1311
Size: 416 895
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 70 971 1311
Size: 70 901 340
Fragments arranged by size:
901 340 70
TatI w'GTAC_w
Cuts at: 0 1019 1311
Size: 1019 292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 45 77 1311
Size: 45 32 1234
Fragments arranged by size:
1234 45 32
XmnI GAAnn'nnTTC
Cuts at: 0 583 667 919 1003 1171 1208 1255 1311
Size: 583 84 252 84 168 37 47 56
Fragments arranged by size:
583 252 168 84 84 56 47 37
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApoI AseI BbsI BglII
BmrI BplI BpmI BpuEI BsaBI BsaWI BsgI BsmI
BspEI BspHI BsrDI BstBI BstYI BtsI CspCI EarI
Eco57MI EcoNI EcoRI HindIII MfeI MslI NdeI NspI
PciI PfoI PsiI SmlI StuI StyI TaqII TatI
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI
Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI
XmaI ZraI