Restriction map for
pF1KB8809
(Linear) (Six-base) MAPSORT of: KIBB8809.seq Check: 2070 from: 1 to: 1080
KIBB8809.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 1080
Size: 802 278
AlwNI CAG_nnn'CTG
Cuts at: 0 493 1080
Size: 493 587
ApaLI G'TGCA_C
Cuts at: 0 418 1080
Size: 418 662
ApoI r'AATT_y
Cuts at: 0 168 928 1007 1080
Size: 168 760 79 73
Fragments arranged by size:
760 168 79 73
BanII G_rGCy'C
Cuts at: 0 119 1001 1080
Size: 119 882 79
Fragments arranged by size:
882 119 79
BciVI GTATCCnnnnn_n'
Cuts at: 0 82 1080
Size: 82 998
BclI T'GATC_A
Cuts at: 0 671 1080
Size: 671 409
BglI GCCn_nnn'nGGC
Cuts at: 0 859 1080
Size: 859 221
Bme1580I G_kGCm'C
Cuts at: 0 422 1080
Size: 422 658
BmgBI CAC'GTC
Cuts at: 0 590 1080
Size: 590 490
Bpu10I CC'TnA_GC
Cuts at: 0 208 1080
Size: 208 872
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 1080
Size: 456 624
BsaI GGTCTCn'nnnn_
Cuts at: 0 787 1080
Size: 787 293
BsaBI GATnn'nnATC
Cuts at: 0 1061 1080
Size: 1061 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 991 1080
Size: 991 89
BseYI C'CCAG_C
Cuts at: 0 1027 1080
Size: 1027 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 810 1080
Size: 810 270
BsiHKAI G_wGCw'C
Cuts at: 0 119 422 785 983 1080
Size: 119 303 363 198 97
Fragments arranged by size:
363 303 198 119 97
Bsp1286I G_dGCh'C
Cuts at: 0 119 422 785 983 1001 1080
Size: 119 303 363 198 18 79
Fragments arranged by size:
363 303 198 119 79 18
BspHI T'CATG_A
Cuts at: 0 389 1059 1080
Size: 389 670 21
Fragments arranged by size:
670 389 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 970 1080
Size: 239 731 110
Fragments arranged by size:
731 239 110
BstXI CCAn_nnnn'nTGG
Cuts at: 0 994 1080
Size: 994 86
BstYI r'GATC_y
Cuts at: 0 252 1080
Size: 252 828
Bsu36I CC'TnA_GG
Cuts at: 0 642 1080
Size: 642 438
BtgI C'CryG_G
Cuts at: 0 8 737 1052 1080
Size: 8 729 315 28
Fragments arranged by size:
729 315 28 8
BtsI GCAGTG_nn'
Cuts at: 0 885 1080
Size: 885 195
DraIII CAC_nnn'GTG
Cuts at: 0 426 590 1080
Size: 426 164 490
Fragments arranged by size:
490 426 164
EarI CTCTTCn'nnn_
Cuts at: 0 124 265 754 790 958 1080
Size: 124 141 489 36 168 122
Fragments arranged by size:
489 168 141 124 122 36
EcoICRI GAG'CTC
Cuts at: 0 117 1080
Size: 117 963
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 1080
Size: 802 278
EcoO109I rG'GnC_Cy
Cuts at: 0 446 680 969 1080
Size: 446 234 289 111
Fragments arranged by size:
446 289 234 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 747 1080
Size: 747 333
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 620 652 1080
Size: 620 32 428
Fragments arranged by size:
620 428 32
MfeI C'AATT_G
Cuts at: 0 413 1080
Size: 413 667
MslI CAynn'nnrTG
Cuts at: 0 388 1080
Size: 388 692
NcoI C'CATG_G
Cuts at: 0 1052 1080
Size: 1052 28
PflMI CCAn_nnn'nTGG
Cuts at: 0 590 1080
Size: 590 490
PfoI T'CCnGG_A
Cuts at: 0 340 1080
Size: 340 740
PpuMI rG'GwC_Cy
Cuts at: 0 446 680 1080
Size: 446 234 400
Fragments arranged by size:
446 400 234
PstI C_TGCA'G
Cuts at: 0 967 1080
Size: 967 113
SacI G_AGCT'C
Cuts at: 0 119 1080
Size: 119 961
SapI GCTCTTCn'nnn_
Cuts at: 0 124 265 790 958 1080
Size: 124 141 525 168 122
Fragments arranged by size:
525 168 141 124 122
SbfI CC_TGCA'GG
Cuts at: 0 967 1080
Size: 967 113
ScaI AGT'ACT
Cuts at: 0 571 1080
Size: 571 509
SfcI C'TryA_G
Cuts at: 0 946 963 1080
Size: 946 17 117
Fragments arranged by size:
946 117 17
SmlI C'TyrA_G
Cuts at: 0 435 1080
Size: 435 645
StyI C'CwwG_G
Cuts at: 0 140 753 1052 1080
Size: 140 613 299 28
Fragments arranged by size:
613 299 140 28
TatI w'GTAC_w
Cuts at: 0 347 569 938 1080
Size: 347 222 369 142
Fragments arranged by size:
369 347 222 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 158 190 1080
Size: 158 32 890
Fragments arranged by size:
890 158 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 310 870 1080
Size: 310 560 210
Fragments arranged by size:
560 310 210
XmnI GAAnn'nnTTC
Cuts at: 0 815 1080
Size: 815 265
Enzymes that do cut:
AcuI AlwNI ApaLI ApoI BanII BciVI BclI BglI
Bme1580I BmgBI Bpu10I BpuEI BsaI BsaBI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BstXI BstYI Bsu36I
BtgI BtsI DraIII EarI EcoICRI Eco57MI EcoO109I FalI
Hin4I MfeI MslI NcoI PflMI PfoI PpuMI PstI
SacI SapI SbfI ScaI SfcI SmlI StyI TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BfrBI BglII BlpI BmrI BmtI BplI
BpmI BsaAI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DrdI EaeI EagI EciI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XhoI XmaI
ZraI