(Linear) (Six-base) MAPSORT of: KIBB8808.seq  Check: 7350  from: 1  to: 1065



KIBB8808.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AccI GT'mk_AC

Cuts at:      0     215    1052    1065 
   Size:        215     837      13

  Fragments arranged by size: 

                 837     215      13
 
Acc65I G'GTAC_C

Cuts at:      0     392    1065 
   Size:        392     673
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     548     694    1065 
   Size:        548     146     371

  Fragments arranged by size: 

                 548     371     146
 
AleI CACnn'nnGTG

Cuts at:      0     878    1065 
   Size:        878     187
 
AvrII C'CTAG_G

Cuts at:      0     975     991    1065 
   Size:        975      16      74

  Fragments arranged by size: 

                 975      74      16
 
BanI G'GyrC_C

Cuts at:      0     392    1065 
   Size:        392     673
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     139     246     987    1065 
   Size:        139     107     741      78

  Fragments arranged by size: 

                 741     139     107      78
 
BbvCI CC'TCA_GC

Cuts at:      0     196    1065 
   Size:        196     869
 
BglI GCCn_nnn'nGGC

Cuts at:      0     487    1065 
   Size:        487     578
 
BglII A'GATC_T

Cuts at:      0     584    1065 
   Size:        584     481
 
Bme1580I G_kGCm'C

Cuts at:      0     451    1065 
   Size:        451     614
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1029    1065 
   Size:       1029      36
 
Bpu10I CC'TnA_GC

Cuts at:      0     196    1065 
   Size:        196     869
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48    1065 
   Size:         48    1017
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     213    1023    1065 
   Size:        213     810      42

  Fragments arranged by size: 

                 810     213      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     649    1065 
   Size:        649     416
 
BsiHKAI G_wGCw'C

Cuts at:      0     722    1065 
   Size:        722     343
 
Bsp1286I G_dGCh'C

Cuts at:      0     451     722    1065 
   Size:        451     271     343

  Fragments arranged by size: 

                 451     343     271
 
BspHI T'CATG_A

Cuts at:      0     377    1065 
   Size:        377     688
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     251    1065 
   Size:        251     814
 
BsrDI GCAATG_nn'

Cuts at:      0     499     731    1065 
   Size:        499     232     334

  Fragments arranged by size: 

                 499     334     232
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     795    1065 
   Size:        795     270
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     714    1065 
   Size:        714     351
 
BstYI r'GATC_y

Cuts at:      0     584    1065 
   Size:        584     481
 
BstZ17I GTA'TAC

Cuts at:      0     216    1065 
   Size:        216     849
 
BtsI GCAGTG_nn'

Cuts at:      0     873    1065 
   Size:        873     192
 
EarI CTCTTCn'nnn_

Cuts at:      0     670    1065 
   Size:        670     395
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     548     694    1029    1065 
   Size:        548     146     335      36

  Fragments arranged by size: 

                 548     335     146      36
 
EcoO109I rG'GnC_Cy

Cuts at:      0     753    1065 
   Size:        753     312
 
EcoRV GAT'ATC

Cuts at:      0      57    1065 
   Size:         57    1008
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      46      78     264     296    1065 
   Size:         46      32     186      32     769

  Fragments arranged by size: 

                 769     186      46      32      32
 
KpnI G_GTAC'C

Cuts at:      0     396    1065 
   Size:        396     669
 
MslI CAynn'nnrTG

Cuts at:      0     376     382     640     878    1065 
   Size:        376       6     258     238     187

  Fragments arranged by size: 

                 376     258     238     187       6
 
MspA1I CmG'CkG

Cuts at:      0     672     831    1065 
   Size:        672     159     234

  Fragments arranged by size: 

                 672     234     159
 
NspI r_CATG'y

Cuts at:      0     363    1065 
   Size:        363     702
 
PpuMI rG'GwC_Cy

Cuts at:      0     753    1065 
   Size:        753     312
 
PvuII CAG'CTG

Cuts at:      0     672     831    1065 
   Size:        672     159     234

  Fragments arranged by size: 

                 672     234     159
 
SanDI GG'GwC_CC

Cuts at:      0     753    1065 
   Size:        753     312
 
SfcI C'TryA_G

Cuts at:      0     351     541    1065 
   Size:        351     190     524

  Fragments arranged by size: 

                 524     351     190
 
SmlI C'TyrA_G

Cuts at:      0      63    1065 
   Size:         63    1002
 
SspI AAT'ATT

Cuts at:      0     941    1065 
   Size:        941     124
 
StyI C'CwwG_G

Cuts at:      0     701     975     991    1065 
   Size:        701     274      16      74

  Fragments arranged by size: 

                 701     274      74      16
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      52     285     859    1065 
   Size:         52     233     574     206

  Fragments arranged by size: 

                 574     233     206      52
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     298    1065 
   Size:        298     767
 
XmnI GAAnn'nnTTC

Cuts at:      0      86     586     941    1065 
   Size:         86     500     355     124

  Fragments arranged by size: 

                 500     355     124      86
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AleI    AvrII     BanI     BbsI    BbvCI
     BglI    BglII Bme1580I     BpmI   Bpu10I    BpuEI    BseRI     BsgI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI   BstAPI    BstXI    BstYI
  BstZ17I     BtsI     EarI  Eco57MI EcoO109I    EcoRV    Hin4I     KpnI
     MslI   MspA1I     NspI    PpuMI    PvuII    SanDI     SfcI     SmlI
     SspI     StyI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI    BanII     BbeI     BcgI    BciVI
     BclI    BfrBI     BlpI    BmgBI     BmrI     BmtI     BplI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI