Restriction map for
pF1KB8807
(Linear) (Six-base) MAPSORT of: KIBB8807.seq Check: 5656 from: 1 to: 1065
KIBB8807.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
Acc65I G'GTAC_C
Cuts at: 0 34 392 1065
Size: 34 358 673
Fragments arranged by size:
673 358 34
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 1065
Size: 542 523
ApoI r'AATT_y
Cuts at: 0 502 1065
Size: 502 563
BamHI G'GATC_C
Cuts at: 0 269 1065
Size: 269 796
BanI G'GyrC_C
Cuts at: 0 34 392 683 1065
Size: 34 358 291 382
Fragments arranged by size:
382 358 291 34
BanII G_rGCy'C
Cuts at: 0 432 1065
Size: 432 633
BbvCI CC'TCA_GC
Cuts at: 0 679 1065
Size: 679 386
BciVI GTATCCnnnnn_n'
Cuts at: 0 561 1065
Size: 561 504
BglI GCCn_nnn'nGGC
Cuts at: 0 149 1065
Size: 149 916
BglII A'GATC_T
Cuts at: 0 359 1065
Size: 359 706
Bme1580I G_kGCm'C
Cuts at: 0 113 688 1065
Size: 113 575 377
Fragments arranged by size:
575 377 113
BmgBI CAC'GTC
Cuts at: 0 57 1065
Size: 57 1008
BmrI ACTGGGnnnn_n'
Cuts at: 0 96 303 683 1065
Size: 96 207 380 382
Fragments arranged by size:
382 380 207 96
BmtI G_CTAG'C
Cuts at: 0 490 1065
Size: 490 575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 603 1065
Size: 603 462
Bpu10I CC'TnA_GC
Cuts at: 0 679 1065
Size: 679 386
BsaI GGTCTCn'nnnn_
Cuts at: 0 19 1065
Size: 19 1046
BsaWI w'CCGG_w
Cuts at: 0 923 1065
Size: 923 142
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 313 1065
Size: 313 752
BsiHKAI G_wGCw'C
Cuts at: 0 93 555 1065
Size: 93 462 510
Fragments arranged by size:
510 462 93
Bsp1286I G_dGCh'C
Cuts at: 0 93 113 432 555 688 1065
Size: 93 20 319 123 133 377
Fragments arranged by size:
377 319 133 123 93 20
BspEI T'CCGG_A
Cuts at: 0 923 1065
Size: 923 142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 248 941 1065
Size: 224 24 693 124
Fragments arranged by size:
693 224 124 24
BsrBI CCG'CTC
Cuts at: 0 822 1065
Size: 822 243
BsrDI GCAATG_nn'
Cuts at: 0 159 1065
Size: 159 906
BsrGI T'GTAC_A
Cuts at: 0 350 1065
Size: 350 715
BstYI r'GATC_y
Cuts at: 0 269 359 1065
Size: 269 90 706
Fragments arranged by size:
706 269 90
BtgI C'CryG_G
Cuts at: 0 306 1065
Size: 306 759
BtsI GCAGTG_nn'
Cuts at: 0 488 555 876 1065
Size: 488 67 321 189
Fragments arranged by size:
488 321 189 67
EaeI y'GGCC_r
Cuts at: 0 227 303 398 644 1065
Size: 227 76 95 246 421
Fragments arranged by size:
421 246 227 95 76
EarI CTCTTCn'nnn_
Cuts at: 0 250 500 518 530 789 1065
Size: 250 250 18 12 259 276
Fragments arranged by size:
276 259 250 250 18 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 603 1065
Size: 542 61 462
Fragments arranged by size:
542 462 61
EcoO109I rG'GnC_Cy
Cuts at: 0 474 1065
Size: 474 591
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 559 1065
Size: 559 506
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 816 848 1065
Size: 816 32 217
Fragments arranged by size:
816 217 32
KpnI G_GTAC'C
Cuts at: 0 38 396 1065
Size: 38 358 669
Fragments arranged by size:
669 358 38
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 891 1065
Size: 891 174
MscI TGG'CCA
Cuts at: 0 229 305 400 646 1065
Size: 229 76 95 246 419
Fragments arranged by size:
419 246 229 95 76
MslI CAynn'nnrTG
Cuts at: 0 736 850 1065
Size: 736 114 215
Fragments arranged by size:
736 215 114
MspA1I CmG'CkG
Cuts at: 0 117 1065
Size: 117 948
NcoI C'CATG_G
Cuts at: 0 306 1065
Size: 306 759
NheI G'CTAG_C
Cuts at: 0 486 1065
Size: 486 579
NspI r_CATG'y
Cuts at: 0 315 1065
Size: 315 750
SapI GCTCTTCn'nnn_
Cuts at: 0 250 500 1065
Size: 250 250 565
Fragments arranged by size:
565 250 250
SexAI A'CCwGG_T
Cuts at: 0 839 1065
Size: 839 226
StyI C'CwwG_G
Cuts at: 0 306 1065
Size: 306 759
TatI w'GTAC_w
Cuts at: 0 119 350 701 1065
Size: 119 231 351 364
Fragments arranged by size:
364 351 231 119
Tth111I GACn'n_nGTC
Cuts at: 0 841 1065
Size: 841 224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 93 1065
Size: 93 972
Enzymes that do cut:
Acc65I AcuI ApoI BamHI BanI BanII BbvCI BciVI
BglI BglII Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BsaI BsaWI BseRI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrGI BstYI BtgI BtsI EaeI EarI Eco57MI
EcoO109I FalI Hin4I KpnI MmeI MscI MslI MspA1I
NcoI NheI NspI SapI SexAI StyI TatI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BbeI BbsI BcgI
BclI BfrBI BlpI BplI BpuEI BsaAI BsaBI BsaHI
BsaXI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspHI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI XbaI XhoI
XmaI XmnI ZraI