Restriction map for
pF1KB8804
(Linear) (Six-base) MAPSORT of: KIBB8804.seq Check: 5259 from: 1 to: 1050
KIBB8804.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1050
Size: 329 721
AlwNI CAG_nnn'CTG
Cuts at: 0 387 1050
Size: 387 663
ApoI r'AATT_y
Cuts at: 0 112 243 1050
Size: 112 131 807
Fragments arranged by size:
807 131 112
AvrII C'CTAG_G
Cuts at: 0 573 1050
Size: 573 477
BbsI GAAGACnn'nnnn_
Cuts at: 0 981 1050
Size: 981 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 828 862 1050
Size: 828 34 188
Fragments arranged by size:
828 188 34
BclI T'GATC_A
Cuts at: 0 812 1050
Size: 812 238
BglII A'GATC_T
Cuts at: 0 611 1050
Size: 611 439
BlpI GC'TnA_GC
Cuts at: 0 502 1050
Size: 502 548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 990 1050
Size: 990 60
BsaBI GATnn'nnATC
Cuts at: 0 91 1050
Size: 91 959
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1009 1050
Size: 1009 41
BseYI C'CCAG_C
Cuts at: 0 433 512 1050
Size: 433 79 538
Fragments arranged by size:
538 433 79
BsmI GAATG_Cn'
Cuts at: 0 383 551 1050
Size: 383 168 499
Fragments arranged by size:
499 383 168
BspHI T'CATG_A
Cuts at: 0 680 1050
Size: 680 370
BsrGI T'GTAC_A
Cuts at: 0 233 353 1050
Size: 233 120 697
Fragments arranged by size:
697 233 120
BstAPI GCAn_nnn'nTGC
Cuts at: 0 196 1050
Size: 196 854
BstXI CCAn_nnnn'nTGG
Cuts at: 0 186 1050
Size: 186 864
BstYI r'GATC_y
Cuts at: 0 611 1050
Size: 611 439
BtgI C'CryG_G
Cuts at: 0 179 1050
Size: 179 871
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 831 855 1050
Size: 831 24 195
Fragments arranged by size:
831 195 24
EaeI y'GGCC_r
Cuts at: 0 950 1050
Size: 950 100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 990 1050
Size: 329 661 60
Fragments arranged by size:
661 329 60
EcoO109I rG'GnC_Cy
Cuts at: 0 965 1018 1050
Size: 965 53 32
Fragments arranged by size:
965 53 32
HincII GTy'rAC
Cuts at: 0 23 1050
Size: 23 1027
MscI TGG'CCA
Cuts at: 0 952 1050
Size: 952 98
MslI CAynn'nnrTG
Cuts at: 0 184 685 1050
Size: 184 501 365
Fragments arranged by size:
501 365 184
MspA1I CmG'CkG
Cuts at: 0 516 821 887 1050
Size: 516 305 66 163
Fragments arranged by size:
516 305 163 66
NcoI C'CATG_G
Cuts at: 0 179 1050
Size: 179 871
PfoI T'CCnGG_A
Cuts at: 0 967 1050
Size: 967 83
PpuMI rG'GwC_Cy
Cuts at: 0 965 1018 1050
Size: 965 53 32
Fragments arranged by size:
965 53 32
PshAI GACnn'nnGTC
Cuts at: 0 71 1050
Size: 71 979
PsiI TTA'TAA
Cuts at: 0 767 1050
Size: 767 283
PvuII CAG'CTG
Cuts at: 0 516 821 887 1050
Size: 516 305 66 163
Fragments arranged by size:
516 305 163 66
ScaI AGT'ACT
Cuts at: 0 1038 1050
Size: 1038 12
SfcI C'TryA_G
Cuts at: 0 69 251 1022 1050
Size: 69 182 771 28
Fragments arranged by size:
771 182 69 28
StyI C'CwwG_G
Cuts at: 0 179 573 1050
Size: 179 394 477
Fragments arranged by size:
477 394 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 299 1050
Size: 299 751
TatI w'GTAC_w
Cuts at: 0 233 353 1036 1050
Size: 233 120 683 14
Fragments arranged by size:
683 233 120 14
Enzymes that do cut:
AcuI AlwNI ApoI AvrII BbsI BcgI BclI BglII
BlpI BpmI BsaBI BseRI BseYI BsmI BspHI BsrGI
BstAPI BstXI BstYI BtgI BtgZI EaeI Eco57MI EcoO109I
HincII MscI MslI MspA1I NcoI PfoI PpuMI PshAI
PsiI PvuII ScaI SfcI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbvCI BciVI BfrBI BglI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI