(Linear) (Six-base) MAPSORT of: KIBB8803.seq  Check: 2263  from: 1  to: 1047



KIBB8803.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508     698    1047 
   Size:        508     190     349

  Fragments arranged by size: 

                 508     349     190
 
AhdI GACnn_n'nnGTC

Cuts at:      0     550    1047 
   Size:        550     497
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     740     772    1047 
   Size:        740      32     275

  Fragments arranged by size: 

                 740     275      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1029    1047 
   Size:       1029      18
 
ApoI r'AATT_y

Cuts at:      0     173    1047 
   Size:        173     874
 
AvaI C'yCGr_G

Cuts at:      0     116    1047 
   Size:        116     931
 
BanII G_rGCy'C

Cuts at:      0     476     708    1047 
   Size:        476     232     339

  Fragments arranged by size: 

                 476     339     232
 
BglI GCCn_nnn'nGGC

Cuts at:      0     772    1047 
   Size:        772     275
 
Bme1580I G_kGCm'C

Cuts at:      0     539     652    1047 
   Size:        539     113     395

  Fragments arranged by size: 

                 539     395     113
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     985    1047 
   Size:        985      62
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     622     728    1047 
   Size:        622     106     319

  Fragments arranged by size: 

                 622     319     106
 
Bpu10I CC'TnA_GC

Cuts at:      0    1022    1047 
   Size:       1022      25
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     173     563     910    1047 
   Size:        160      13     390     347     137

  Fragments arranged by size: 

                 390     347     160     137      13
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     113     259    1047 
   Size:        113     146     788

  Fragments arranged by size: 

                 788     146     113
 
BsaBI GATnn'nnATC

Cuts at:      0     325    1047 
   Size:        325     722
 
BsaHI Gr'CG_yC

Cuts at:      0       7    1047 
   Size:          7    1040
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     484     514    1047 
   Size:        484      30     533

  Fragments arranged by size: 

                 533     484      30
 
BseYI C'CCAG_C

Cuts at:      0     667    1047 
   Size:        667     380
 
BsiHKAI G_wGCw'C

Cuts at:      0     476    1047 
   Size:        476     571
 
Bsp1286I G_dGCh'C

Cuts at:      0     476     539     652     708    1047 
   Size:        476      63     113      56     339

  Fragments arranged by size: 

                 476     339     113      63      56
 
BsrBI CCG'CTC

Cuts at:      0     840    1047 
   Size:        840     207
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     419    1047 
   Size:        419     628
 
BstYI r'GATC_y

Cuts at:      0     159     945    1047 
   Size:        159     786     102

  Fragments arranged by size: 

                 786     159     102
 
BtgI C'CryG_G

Cuts at:      0     330     336     448    1047 
   Size:        330       6     112     599

  Fragments arranged by size: 

                 599     330     112       6
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      40      75    1047 
   Size:         40      35     972

  Fragments arranged by size: 

                 972      40      35
 
DraI TTT'AAA

Cuts at:      0     857    1047 
   Size:        857     190
 
EaeI y'GGCC_r

Cuts at:      0     333     626     849    1047 
   Size:        333     293     223     198

  Fragments arranged by size: 

                 333     293     223     198
 
EarI CTCTTCn'nnn_

Cuts at:      0     713    1047 
   Size:        713     334
 
EcoICRI GAG'CTC

Cuts at:      0     474    1047 
   Size:        474     573
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508     622     698     728    1047 
   Size:        508     114      76      30     319

  Fragments arranged by size: 

                 508     319     114      76      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     928    1047 
   Size:        928     119
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     538    1047 
   Size:        538     509
 
FspI TGC'GCA

Cuts at:      0    1033    1047 
   Size:       1033      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     536     568    1047 
   Size:        536      32     479

  Fragments arranged by size: 

                 536     479      32
 
HindIII A'AGCT_T

Cuts at:      0     275    1047 
   Size:        275     772
 
MfeI C'AATT_G

Cuts at:      0      23    1047 
   Size:         23    1024
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     815    1047 
   Size:        815     232
 
MscI TGG'CCA

Cuts at:      0     335     628     851    1047 
   Size:        335     293     223     196

  Fragments arranged by size: 

                 335     293     223     196
 
MspA1I CmG'CkG

Cuts at:      0     528    1047 
   Size:        528     519
 
NcoI C'CATG_G

Cuts at:      0     336     448    1047 
   Size:        336     112     599

  Fragments arranged by size: 

                 599     336     112
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     342    1047 
   Size:        342     705
 
PvuII CAG'CTG

Cuts at:      0     528    1047 
   Size:        528     519
 
SacI G_AGCT'C

Cuts at:      0     476    1047 
   Size:        476     571
 
SapI GCTCTTCn'nnn_

Cuts at:      0     713    1047 
   Size:        713     334
 
SexAI A'CCwGG_T

Cuts at:      0     735    1047 
   Size:        735     312
 
SmlI C'TyrA_G

Cuts at:      0     139     188     578     925    1047 
   Size:        139      49     390     347     122

  Fragments arranged by size: 

                 390     347     139     122      49
 
StuI AGG'CCT

Cuts at:      0     862    1047 
   Size:        862     185
 
StyI C'CwwG_G

Cuts at:      0     336     448     894    1047 
   Size:        336     112     446     153

  Fragments arranged by size: 

                 446     336     153     112
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     484     516    1047 
   Size:        484      32     531

  Fragments arranged by size: 

                 531     484      32
 
XbaI T'CTAG_A

Cuts at:      0     177    1047 
   Size:        177     870
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     892    1047 
   Size:        892     155
 
XmnI GAAnn'nnTTC

Cuts at:      0     659    1047 
   Size:        659     388
 

 Enzymes that do cut:

     AcuI     AhdI     AloI    AlwNI     ApoI     AvaI    BanII     BglI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaXI    BseYI  BsiHKAI Bsp1286I    BsrBI    BstXI    BstYI     BtgI
    CspCI     DraI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI     FalI
     FspI    Hin4I  HindIII     MfeI     MmeI     MscI   MspA1I     NcoI
    PflMI    PvuII     SacI     SapI    SexAI     SmlI     StuI     StyI
     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI
    BsaAI    BsaWI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
   DraIII     DrdI     EagI     EciI EcoO109I    EcoRI    EcoRV     FseI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MluI     MslI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TatI  Tth111I     XhoI     XmaI     ZraI