(Linear) (Six-base) MAPSORT of: KIBB8788.seq  Check: 1474  from: 1  to: 900



KIBB8788.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     238     900 
   Size:        238     662
 
AlwNI CAG_nnn'CTG

Cuts at:      0     248     900 
   Size:        248     652
 
ApaI G_GGCC'C

Cuts at:      0     275     900 
   Size:        275     625
 
AvaI C'yCGr_G

Cuts at:      0     152     900 
   Size:        152     748
 
BanII G_rGCy'C

Cuts at:      0     200     275     900 
   Size:        200      75     625

  Fragments arranged by size: 

                 625     200      75
 
BclI T'GATC_A

Cuts at:      0     341     900 
   Size:        341     559
 
Bme1580I G_kGCm'C

Cuts at:      0     275     900 
   Size:        275     625
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      36     900 
   Size:         36     864
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     900 
   Size:        176     724
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     164     900 
   Size:        164     736
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     249     331     334     900 
   Size:        249      82       3     566

  Fragments arranged by size: 

                 566     249      82       3
 
BseYI C'CCAG_C

Cuts at:      0     300     900 
   Size:        300     600
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     565     598     900 
   Size:        565      33     302

  Fragments arranged by size: 

                 565     302      33
 
Bsp1286I G_dGCh'C

Cuts at:      0     200     275     900 
   Size:        200      75     625

  Fragments arranged by size: 

                 625     200      75
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     238     900 
   Size:        238     662
 
BsrBI CCG'CTC

Cuts at:      0      20     900 
   Size:         20     880
 
Bsu36I CC'TnA_GG

Cuts at:      0      25     900 
   Size:         25     875
 
BtsI GCAGTG_nn'

Cuts at:      0     586     900 
   Size:        586     314
 
DraI TTT'AAA

Cuts at:      0     819     900 
   Size:        819      81
 
EarI CTCTTCn'nnn_

Cuts at:      0     398     644     701     900 
   Size:        398     246      57     199

  Fragments arranged by size: 

                 398     246     199      57
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     900 
   Size:        176     724
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      88     900 
   Size:         88     812
 
EcoO109I rG'GnC_Cy

Cuts at:      0     271     272     900 
   Size:        271       1     628

  Fragments arranged by size: 

                 628     271       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     369     900 
   Size:        369     531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      80     112     900 
   Size:         80      32     788

  Fragments arranged by size: 

                 788      80      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     389     900 
   Size:        389     511
 
MspA1I CmG'CkG

Cuts at:      0     248     488     900 
   Size:        248     240     412

  Fragments arranged by size: 

                 412     248     240
 
PasI CC'CwG_GG

Cuts at:      0     268     900 
   Size:        268     632
 
PspOMI G'GGCC_C

Cuts at:      0     271     900 
   Size:        271     629
 
SmaI CCC'GGG

Cuts at:      0     154     900 
   Size:        154     746
 
SpeI A'CTAG_T

Cuts at:      0     699     900 
   Size:        699     201
 
StyI C'CwwG_G

Cuts at:      0      84     209     900 
   Size:         84     125     691

  Fragments arranged by size: 

                 691     125      84
 
XbaI T'CTAG_A

Cuts at:      0     106     900 
   Size:        106     794
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     216     375     900 
   Size:        216     159     525

  Fragments arranged by size: 

                 525     216     159
 
XmaI C'CCGG_G

Cuts at:      0     152     900 
   Size:        152     748
 

 Enzymes that do cut:

     AarI    AlwNI     ApaI     AvaI    BanII     BclI Bme1580I     BplI
     BpmI     BsaI    BseRI    BseYI     BsgI Bsp1286I    BspMI    BsrBI
   Bsu36I     BtsI     DraI     EarI  Eco57MI    EcoNI EcoO109I     FalI
    Hin4I     MmeI   MspA1I     PasI   PspOMI     SmaI     SpeI     StyI
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I     BtgI    BtgZI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XhoI     XmnI
     ZraI