(Linear) (Six-base) MAPSORT of: KIBB8748.seq  Check: 6806  from: 1  to: 507



KIBB8748.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      48     507 
   Size:         48     459
 
Acc65I G'GTAC_C

Cuts at:      0     444     507 
   Size:        444      63
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     428     461     507 
   Size:        428      33      46

  Fragments arranged by size: 

                 428      46      33
 
BanI G'GyrC_C

Cuts at:      0     385     444     507 
   Size:        385      59      63

  Fragments arranged by size: 

                 385      63      59
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     499     507 
   Size:        499       8
 
BbvCI CC'TCA_GC

Cuts at:      0      41     507 
   Size:         41     466
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     403     507 
   Size:        403     104
 
BlpI GC'TnA_GC

Cuts at:      0     485     507 
   Size:        485      22
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     507 
   Size:        458      49
 
Bpu10I CC'TnA_GC

Cuts at:      0      41     185     209     233     257     281     305     377 
   Size:         41     144      24      24      24      24      24      72

Cuts at:    377     401     507 
   Size:         24     106

  Fragments arranged by size: 

                 144     106      72      41      24      24      24      24
                  24      24
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     456     486     507 
   Size:        456      30      21

  Fragments arranged by size: 

                 456      30      21
 
BseYI C'CCAG_C

Cuts at:      0      70     480     507 
   Size:         70     410      27

  Fragments arranged by size: 

                 410      70      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      48     507 
   Size:         48     459
 
Bsu36I CC'TnA_GG

Cuts at:      0      86     507 
   Size:         86     421
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     507 
   Size:        458      49
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     188     212     236     260     284     308     332     356 
   Size:        188      24      24      24      24      24      24      24

Cuts at:    356     380     404     428     507 
   Size:         24      24      24      79

  Fragments arranged by size: 

                 188      79      24      24      24      24      24      24
                  24      24      24      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0     180     204     228     252     276     300     324     348 
   Size:        180      24      24      24      24      24      24      24

Cuts at:    348     507 
   Size:        159

  Fragments arranged by size: 

                 180     159      24      24      24      24      24      24
                  24
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      42     507 
   Size:         42     465
 
KpnI G_GTAC'C

Cuts at:      0     448     507 
   Size:        448      59
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     142     507 
   Size:        142     365
 
PpuMI rG'GwC_Cy

Cuts at:      0     180     204     228     252     276     300     324     348 
   Size:        180      24      24      24      24      24      24      24

Cuts at:    348     507 
   Size:        159

  Fragments arranged by size: 

                 180     159      24      24      24      24      24      24
                  24
 
SspI AAT'ATT

Cuts at:      0      95     507 
   Size:         95     412
 
StyI C'CwwG_G

Cuts at:      0     110     176     200     224     248     272     296     320 
   Size:        110      66      24      24      24      24      24      24

Cuts at:    320     357     507 
   Size:         37     150

  Fragments arranged by size: 

                 150     110      66      37      24      24      24      24
                  24      24
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     141     173     405     437     507 
   Size:        141      32     232      32      70

  Fragments arranged by size: 

                 232     141      70      32      32
 

 Enzymes that do cut:

     AarI   Acc65I     BaeI     BanI     BbsI    BbvCI    BciVI     BlpI
     BpmI   Bpu10I    BsaXI    BseYI    BspMI   Bsu36I  Eco57MI    EcoNI
 EcoO109I     FalI     KpnI    PflMI    PpuMI     SspI     StyI     TstI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII    BamHI    BanII     BbeI
     BcgI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI