Restriction map for
pF1KB8746
(Linear) (Six-base) MAPSORT of: KIBB8746.seq Check: 210 from: 1 to: 531
KIBB8746.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 227 531
Size: 227 304
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 531
Size: 172 359
AvaI C'yCGr_G
Cuts at: 0 201 313 435 531
Size: 201 112 122 96
Fragments arranged by size:
201 122 112 96
BanII G_rGCy'C
Cuts at: 0 18 531
Size: 18 513
BglI GCCn_nnn'nGGC
Cuts at: 0 148 371 531
Size: 148 223 160
Fragments arranged by size:
223 160 148
Bme1580I G_kGCm'C
Cuts at: 0 200 531
Size: 200 331
BmgBI CAC'GTC
Cuts at: 0 453 531
Size: 453 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 531
Size: 319 212
BsaHI Gr'CG_yC
Cuts at: 0 421 531
Size: 421 110
BseYI C'CCAG_C
Cuts at: 0 61 224 346 369 393 477 531
Size: 61 163 122 23 24 84 54
Fragments arranged by size:
163 122 84 61 54 24 23
Bsp1286I G_dGCh'C
Cuts at: 0 18 200 531
Size: 18 182 331
Fragments arranged by size:
331 182 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 82 227 318 531
Size: 82 145 91 213
Fragments arranged by size:
213 145 91 82
BsrFI r'CCGG_y
Cuts at: 0 147 374 531
Size: 147 227 157
Fragments arranged by size:
227 157 147
BtgI C'CryG_G
Cuts at: 0 92 120 360 467 531
Size: 92 28 240 107 64
Fragments arranged by size:
240 107 92 64 28
EaeI y'GGCC_r
Cuts at: 0 173 470 531
Size: 173 297 61
Fragments arranged by size:
297 173 61
EarI CTCTTCn'nnn_
Cuts at: 0 494 531
Size: 494 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 319 531
Size: 172 147 212
Fragments arranged by size:
212 172 147
EcoNI CCTnn'n_nnAGG
Cuts at: 0 325 531
Size: 325 206
EcoO109I rG'GnC_Cy
Cuts at: 0 318 522 531
Size: 318 204 9
Fragments arranged by size:
318 204 9
HaeII r_GCGC'y
Cuts at: 0 460 531
Size: 460 71
MscI TGG'CCA
Cuts at: 0 175 531
Size: 175 356
MspA1I CmG'CkG
Cuts at: 0 228 335 369 393 401 414 531
Size: 228 107 34 24 8 13 117
Fragments arranged by size:
228 117 107 34 24 13 8
NaeI GCC'GGC
Cuts at: 0 149 376 531
Size: 149 227 155
Fragments arranged by size:
227 155 149
NcoI C'CATG_G
Cuts at: 0 92 360 531
Size: 92 268 171
Fragments arranged by size:
268 171 92
NgoMIV G'CCGG_C
Cuts at: 0 147 374 531
Size: 147 227 157
Fragments arranged by size:
227 157 147
PstI C_TGCA'G
Cuts at: 0 115 233 274 329 419 531
Size: 115 118 41 55 90 112
Fragments arranged by size:
118 115 112 90 55 41
PvuII CAG'CTG
Cuts at: 0 228 393 401 414 531
Size: 228 165 8 13 117
Fragments arranged by size:
228 165 117 13 8
SapI GCTCTTCn'nnn_
Cuts at: 0 494 531
Size: 494 37
SfcI C'TryA_G
Cuts at: 0 111 229 270 325 415 531
Size: 111 118 41 55 90 116
Fragments arranged by size:
118 116 111 90 55 41
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 371 531
Size: 371 160
SmaI CCC'GGG
Cuts at: 0 203 437 531
Size: 203 234 94
Fragments arranged by size:
234 203 94
StyI C'CwwG_G
Cuts at: 0 50 92 360 531
Size: 50 42 268 171
Fragments arranged by size:
268 171 50 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 93 417 531
Size: 93 324 114
Fragments arranged by size:
324 114 93
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 522 531
Size: 522 9
XmaI C'CCGG_G
Cuts at: 0 201 435 531
Size: 201 234 96
Fragments arranged by size:
234 201 96
Enzymes that do cut:
AarI AcuI AvaI BanII BglI Bme1580I BmgBI BpmI
BsaHI BseYI Bsp1286I BspMI BsrFI BtgI EaeI EarI
Eco57MI EcoNI EcoO109I HaeII MscI MspA1I NaeI NcoI
NgoMIV PstI PvuII SapI SfcI SfiI SmaI StyI
TaqII XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NarI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XhoI XmnI
ZraI