Restriction map for
pF1KB8737
(Linear) (Six-base) MAPSORT of: KIBB8737.seq Check: 34 from: 1 to: 429
KIBB8737.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 257 429
Size: 257 172
AscI GG'CGCG_CC
Cuts at: 0 140 429
Size: 140 289
AvaI C'yCGr_G
Cuts at: 0 390 429
Size: 390 39
BanI G'GyrC_C
Cuts at: 0 133 384 429
Size: 133 251 45
Fragments arranged by size:
251 133 45
BanII G_rGCy'C
Cuts at: 0 281 429
Size: 281 148
BbeI G_GCGC'C
Cuts at: 0 137 388 429
Size: 137 251 41
Fragments arranged by size:
251 137 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 232 364 429
Size: 232 132 65
Fragments arranged by size:
232 132 65
BglI GCCn_nnn'nGGC
Cuts at: 0 412 429
Size: 412 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 73 429
Size: 73 356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 187 211 429
Size: 55 132 24 218
Fragments arranged by size:
218 132 55 24
BsaHI Gr'CG_yC
Cuts at: 0 134 320 385 429
Size: 134 186 65 44
Fragments arranged by size:
186 134 65 44
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 289 429
Size: 289 140
BseYI C'CCAG_C
Cuts at: 0 291 429
Size: 291 138
BsiEI CG_ry'CG
Cuts at: 0 308 429
Size: 308 121
BsiHKAI G_wGCw'C
Cuts at: 0 281 429
Size: 281 148
Bsp1286I G_dGCh'C
Cuts at: 0 281 429
Size: 281 148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 257 429
Size: 257 172
BsrDI GCAATG_nn'
Cuts at: 0 210 429
Size: 210 219
BsrFI r'CCGG_y
Cuts at: 0 64 95 136 429
Size: 64 31 41 293
Fragments arranged by size:
293 64 41 31
BsrGI T'GTAC_A
Cuts at: 0 90 429
Size: 90 339
BssHII G'CGCG_C
Cuts at: 0 60 140 429
Size: 60 80 289
Fragments arranged by size:
289 80 60
BstYI r'GATC_y
Cuts at: 0 185 429
Size: 185 244
BtgI C'CryG_G
Cuts at: 0 9 371 429
Size: 9 362 58
Fragments arranged by size:
362 58 9
BtsI GCAGTG_nn'
Cuts at: 0 167 429
Size: 167 262
EaeI y'GGCC_r
Cuts at: 0 257 429
Size: 257 172
EcoICRI GAG'CTC
Cuts at: 0 279 429
Size: 279 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 187 211 429
Size: 55 132 24 218
Fragments arranged by size:
218 132 55 24
HaeII r_GCGC'y
Cuts at: 0 137 368 388 429
Size: 137 231 20 41
Fragments arranged by size:
231 137 41 20
KasI G'GCGC_C
Cuts at: 0 133 384 429
Size: 133 251 45
Fragments arranged by size:
251 133 45
MscI TGG'CCA
Cuts at: 0 259 429
Size: 259 170
MspA1I CmG'CkG
Cuts at: 0 11 181 255 429
Size: 11 170 74 174
Fragments arranged by size:
174 170 74 11
NaeI GCC'GGC
Cuts at: 0 66 138 429
Size: 66 72 291
Fragments arranged by size:
291 72 66
NarI GG'CG_CC
Cuts at: 0 134 385 429
Size: 134 251 44
Fragments arranged by size:
251 134 44
NgoMIV G'CCGG_C
Cuts at: 0 64 136 429
Size: 64 72 293
Fragments arranged by size:
293 72 64
PasI CC'CwG_GG
Cuts at: 0 312 429
Size: 312 117
PfoI T'CCnGG_A
Cuts at: 0 188 429
Size: 188 241
PstI C_TGCA'G
Cuts at: 0 254 429
Size: 254 175
PvuI CG_AT'CG
Cuts at: 0 308 429
Size: 308 121
PvuII CAG'CTG
Cuts at: 0 255 429
Size: 255 174
SacI G_AGCT'C
Cuts at: 0 281 429
Size: 281 148
SacII CC_GC'GG
Cuts at: 0 12 429
Size: 12 417
SfcI C'TryA_G
Cuts at: 0 250 429
Size: 250 179
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 412 429
Size: 412 17
SfoI GGC'GCC
Cuts at: 0 135 386 429
Size: 135 251 43
Fragments arranged by size:
251 135 43
SgrAI Cr'CCGG_yG
Cuts at: 0 136 429
Size: 136 293
StuI AGG'CCT
Cuts at: 0 415 429
Size: 415 14
StyI C'CwwG_G
Cuts at: 0 38 429
Size: 38 391
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 424 429
Size: 424 5
TatI w'GTAC_w
Cuts at: 0 90 429
Size: 90 339
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 369 429
Size: 369 60
Enzymes that do cut:
AarI AscI AvaI BanI BanII BbeI BbsI BglI
BmrI BpmI BsaHI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BsrGI BssHII BstYI BtgI BtsI
EaeI EcoICRI Eco57MI HaeII KasI MscI MspA1I NaeI
NarI NgoMIV PasI PfoI PstI PvuI PvuII SacI
SacII SfcI SfiI SfoI SgrAI StuI StyI TaqII
TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BsgI BsiWI BsmI BsmBI BspEI BspHI BsrBI BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MslI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI RsrII SalI SanDI SapI SbfI
ScaI SexAI SgfI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI