Restriction map for
pF1KB8722
(Linear) (Six-base) MAPSORT of: KIBB8722.seq Check: 5509 from: 1 to: 1560
KIBB8722.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AccI GT'mk_AC
Cuts at: 0 149 374 1560
Size: 149 225 1186
Fragments arranged by size:
1186 225 149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1533 1560
Size: 1533 27
AfeI AGC'GCT
Cuts at: 0 741 1560
Size: 741 819
AflII C'TTAA_G
Cuts at: 0 208 1560
Size: 208 1352
AhdI GACnn_n'nnGTC
Cuts at: 0 255 1560
Size: 255 1305
AleI CACnn'nnGTG
Cuts at: 0 1294 1560
Size: 1294 266
AlwNI CAG_nnn'CTG
Cuts at: 0 1556 1560
Size: 1556 4
ApaI G_GGCC'C
Cuts at: 0 1118 1417 1560
Size: 1118 299 143
Fragments arranged by size:
1118 299 143
ApoI r'AATT_y
Cuts at: 0 96 1560
Size: 96 1464
AvaI C'yCGr_G
Cuts at: 0 611 1560
Size: 611 949
BanI G'GyrC_C
Cuts at: 0 952 1560
Size: 952 608
BanII G_rGCy'C
Cuts at: 0 446 632 1118 1417 1490 1560
Size: 446 186 486 299 73 70
Fragments arranged by size:
486 446 299 186 73 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 1354 1560
Size: 1354 206
BbvCI CC'TCA_GC
Cuts at: 0 1096 1560
Size: 1096 464
BclI T'GATC_A
Cuts at: 0 263 1560
Size: 263 1297
BglI GCCn_nnn'nGGC
Cuts at: 0 385 1560
Size: 385 1175
BglII A'GATC_T
Cuts at: 0 81 1560
Size: 81 1479
Bme1580I G_kGCm'C
Cuts at: 0 1118 1417 1560
Size: 1118 299 143
Fragments arranged by size:
1118 299 143
BmrI ACTGGGnnnn_n'
Cuts at: 0 148 304 1044 1523 1560
Size: 148 156 740 479 37
Fragments arranged by size:
740 479 156 148 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 465 1560
Size: 465 1095
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 898 1560
Size: 457 441 662
Fragments arranged by size:
662 457 441
Bpu10I CC'TnA_GC
Cuts at: 0 1096 1560
Size: 1096 464
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1342 1560
Size: 1342 218
BsaI GGTCTCn'nnnn_
Cuts at: 0 601 610 766 1136 1366 1560
Size: 601 9 156 370 230 194
Fragments arranged by size:
601 370 230 194 156 9
BsaAI yAC'GTr
Cuts at: 0 843 1560
Size: 843 717
BsaWI w'CCGG_w
Cuts at: 0 974 1560
Size: 974 586
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1537 1560
Size: 1537 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1285 1330 1560
Size: 1285 45 230
Fragments arranged by size:
1285 230 45
BseYI C'CCAG_C
Cuts at: 0 229 1455 1560
Size: 229 1226 105
Fragments arranged by size:
1226 229 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 835 868 1560
Size: 144 691 33 692
Fragments arranged by size:
692 691 144 33
BsiEI CG_ry'CG
Cuts at: 0 365 1560
Size: 365 1195
BsiHKAI G_wGCw'C
Cuts at: 0 632 1560
Size: 632 928
Bsp1286I G_dGCh'C
Cuts at: 0 446 632 1118 1417 1490 1560
Size: 446 186 486 299 73 70
Fragments arranged by size:
486 446 299 186 73 70
BspEI T'CCGG_A
Cuts at: 0 974 1560
Size: 974 586
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 437 916 1560
Size: 437 479 644
Fragments arranged by size:
644 479 437
BsrBI CCG'CTC
Cuts at: 0 1344 1560
Size: 1344 216
BsrDI GCAATG_nn'
Cuts at: 0 240 1560
Size: 240 1320
BsrFI r'CCGG_y
Cuts at: 0 364 1560
Size: 364 1196
BsrGI T'GTAC_A
Cuts at: 0 32 1560
Size: 32 1528
BstXI CCAn_nnnn'nTGG
Cuts at: 0 489 762 1560
Size: 489 273 798
Fragments arranged by size:
798 489 273
BstYI r'GATC_y
Cuts at: 0 81 967 1274 1560
Size: 81 886 307 286
Fragments arranged by size:
886 307 286 81
Bsu36I CC'TnA_GG
Cuts at: 0 1265 1560
Size: 1265 295
BtgI C'CryG_G
Cuts at: 0 202 1086 1226 1560
Size: 202 884 140 334
Fragments arranged by size:
884 334 202 140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 775 1560
Size: 775 785
BtsI GCAGTG_nn'
Cuts at: 0 120 303 395 885 1560
Size: 120 183 92 490 675
Fragments arranged by size:
675 490 183 120 92
DraIII CAC_nnn'GTG
Cuts at: 0 348 1560
Size: 348 1212
EaeI y'GGCC_r
Cuts at: 0 1103 1560
Size: 1103 457
EarI CTCTTCn'nnn_
Cuts at: 0 551 1378 1478 1560
Size: 551 827 100 82
Fragments arranged by size:
827 551 100 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1417 1560
Size: 1417 143
EcoICRI GAG'CTC
Cuts at: 0 630 1560
Size: 630 930
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 898 1533 1560
Size: 457 441 635 27
Fragments arranged by size:
635 457 441 27
EcoO109I rG'GnC_Cy
Cuts at: 0 257 426 477 573 678 1413 1414 1560
Size: 257 169 51 96 105 735 1 146
Fragments arranged by size:
735 257 169 146 105 96 51 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 291 1560
Size: 291 1269
FspI TGC'GCA
Cuts at: 0 1337 1560
Size: 1337 223
HaeII r_GCGC'y
Cuts at: 0 743 1560
Size: 743 817
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 1537 1560
Size: 241 32 1264 23
Fragments arranged by size:
1264 241 32 23
MscI TGG'CCA
Cuts at: 0 1105 1560
Size: 1105 455
MslI CAynn'nnrTG
Cuts at: 0 62 760 1294 1560
Size: 62 698 534 266
Fragments arranged by size:
698 534 266 62
MspA1I CmG'CkG
Cuts at: 0 790 1088 1560
Size: 790 298 472
Fragments arranged by size:
790 472 298
NcoI C'CATG_G
Cuts at: 0 202 1226 1560
Size: 202 1024 334
Fragments arranged by size:
1024 334 202
PflMI CCAn_nnn'nTGG
Cuts at: 0 348 1437 1560
Size: 348 1089 123
Fragments arranged by size:
1089 348 123
PfoI T'CCnGG_A
Cuts at: 0 422 473 767 1560
Size: 422 51 294 793
Fragments arranged by size:
793 422 294 51
PpuMI rG'GwC_Cy
Cuts at: 0 257 426 477 573 1560
Size: 257 169 51 96 987
Fragments arranged by size:
987 257 169 96 51
PspOMI G'GGCC_C
Cuts at: 0 1114 1413 1560
Size: 1114 299 147
Fragments arranged by size:
1114 299 147
PstI C_TGCA'G
Cuts at: 0 127 140 626 1560
Size: 127 13 486 934
Fragments arranged by size:
934 486 127 13
PvuII CAG'CTG
Cuts at: 0 790 1560
Size: 790 770
SacI G_AGCT'C
Cuts at: 0 632 1560
Size: 632 928
SacII CC_GC'GG
Cuts at: 0 1089 1560
Size: 1089 471
SapI GCTCTTCn'nnn_
Cuts at: 0 1378 1478 1560
Size: 1378 100 82
Fragments arranged by size:
1378 100 82
SbfI CC_TGCA'GG
Cuts at: 0 626 1560
Size: 626 934
SfcI C'TryA_G
Cuts at: 0 123 136 622 1292 1560
Size: 123 13 486 670 268
Fragments arranged by size:
670 486 268 123 13
SmlI C'TyrA_G
Cuts at: 0 208 1357 1560
Size: 208 1149 203
Fragments arranged by size:
1149 208 203
StyI C'CwwG_G
Cuts at: 0 202 990 1226 1560
Size: 202 788 236 334
Fragments arranged by size:
788 334 236 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 355 451 1560
Size: 355 96 1109
Fragments arranged by size:
1109 355 96
TatI w'GTAC_w
Cuts at: 0 32 1445 1560
Size: 32 1413 115
Fragments arranged by size:
1413 115 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1233 1560
Size: 1233 327
XmnI GAAnn'nnTTC
Cuts at: 0 1045 1560
Size: 1045 515
Enzymes that do cut:
AccI AcuI AfeI AflII AhdI AleI AlwNI ApaI
ApoI AvaI BanI BanII BbsI BbvCI BclI BglI
BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I
MscI MslI MspA1I NcoI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SacII SapI SbfI SfcI SmlI
StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BfrBI BlpI BmgBI BmtI BsaBI BsaHI
BsiWI BsmI BsmBI BspHI BssHII BssSI BstAPI BstBI
BstEII BstZ17I ClaI CspCI DraI DrdI EagI EcoNI
EcoRI EcoRV FseI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SanDI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XhoI XmaI ZraI