Restriction map for
pF1KB8711
(Linear) (Six-base) MAPSORT of: KIBB8711.seq Check: 2777 from: 1 to: 1446
KIBB8711.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 1112 1446
Size: 974 138 334
Fragments arranged by size:
974 334 138
AflIII A'CryG_T
Cuts at: 0 138 521 1446
Size: 138 383 925
Fragments arranged by size:
925 383 138
ApoI r'AATT_y
Cuts at: 0 283 784 1446
Size: 283 501 662
Fragments arranged by size:
662 501 283
AseI AT'TA_AT
Cuts at: 0 901 1446
Size: 901 545
BglII A'GATC_T
Cuts at: 0 1130 1446
Size: 1130 316
Bme1580I G_kGCm'C
Cuts at: 0 1220 1446
Size: 1220 226
BmgBI CAC'GTC
Cuts at: 0 522 1446
Size: 522 924
BmrI ACTGGGnnnn_n'
Cuts at: 0 286 1446
Size: 286 1160
BmtI G_CTAG'C
Cuts at: 0 691 1446
Size: 691 755
Bpu10I CC'TnA_GC
Cuts at: 0 1011 1263 1334 1446
Size: 1011 252 71 112
Fragments arranged by size:
1011 252 112 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 245 1446
Size: 32 213 1201
Fragments arranged by size:
1201 213 32
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 512 1446
Size: 512 934
BseYI C'CCAG_C
Cuts at: 0 368 1446
Size: 368 1078
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 904 1446
Size: 501 403 542
Fragments arranged by size:
542 501 403
BsiHKAI G_wGCw'C
Cuts at: 0 504 1446
Size: 504 942
BsmBI CGTCTCn'nnnn_
Cuts at: 0 85 1187 1446
Size: 85 1102 259
Fragments arranged by size:
1102 259 85
Bsp1286I G_dGCh'C
Cuts at: 0 504 1220 1446
Size: 504 716 226
Fragments arranged by size:
716 504 226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1121 1338 1446
Size: 1121 217 108
Fragments arranged by size:
1121 217 108
BstYI r'GATC_y
Cuts at: 0 645 1130 1446
Size: 645 485 316
Fragments arranged by size:
645 485 316
ClaI AT'CG_AT
Cuts at: 0 39 1446
Size: 39 1407
DraI TTT'AAA
Cuts at: 0 797 1446
Size: 797 649
EaeI y'GGCC_r
Cuts at: 0 1197 1446
Size: 1197 249
EarI CTCTTCn'nnn_
Cuts at: 0 833 1148 1375 1446
Size: 833 315 227 71
Fragments arranged by size:
833 315 227 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 1112 1446
Size: 974 138 334
Fragments arranged by size:
974 334 138
EcoO109I rG'GnC_Cy
Cuts at: 0 351 1015 1079 1446
Size: 351 664 64 367
Fragments arranged by size:
664 367 351 64
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 120 610 664 1446
Size: 120 490 54 782
Fragments arranged by size:
782 490 120 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 189 221 656 688 976 1008 1027 1059
Size: 189 32 435 32 288 32 19 32
Cuts at: 1059 1375 1407 1446
Size: 316 32 39
Fragments arranged by size:
435 316 288 189 39 32 32 32
32 32 19
MfeI C'AATT_G
Cuts at: 0 224 1446
Size: 224 1222
MslI CAynn'nnrTG
Cuts at: 0 507 1446
Size: 507 939
MspA1I CmG'CkG
Cuts at: 0 1183 1446
Size: 1183 263
NdeI CA'TA_TG
Cuts at: 0 780 1446
Size: 780 666
NheI G'CTAG_C
Cuts at: 0 687 1446
Size: 687 759
NspI r_CATG'y
Cuts at: 0 142 687 1010 1446
Size: 142 545 323 436
Fragments arranged by size:
545 436 323 142
PasI CC'CwG_GG
Cuts at: 0 23 1230 1446
Size: 23 1207 216
Fragments arranged by size:
1207 216 23
PciI A'CATG_T
Cuts at: 0 138 1446
Size: 138 1308
PflMI CCAn_nnn'nTGG
Cuts at: 0 417 1446
Size: 417 1029
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1147 1179 1446
Size: 1147 32 267
Fragments arranged by size:
1147 267 32
PpuMI rG'GwC_Cy
Cuts at: 0 1015 1079 1446
Size: 1015 64 367
Fragments arranged by size:
1015 367 64
SanDI GG'GwC_CC
Cuts at: 0 1079 1446
Size: 1079 367
SmlI C'TyrA_G
Cuts at: 0 47 260 1446
Size: 47 213 1186
Fragments arranged by size:
1186 213 47
SphI G_CATG'C
Cuts at: 0 1010 1446
Size: 1010 436
SspI AAT'ATT
Cuts at: 0 85 1446
Size: 85 1361
StuI AGG'CCT
Cuts at: 0 103 1315 1446
Size: 103 1212 131
Fragments arranged by size:
1212 131 103
StyI C'CwwG_G
Cuts at: 0 297 1148 1247 1446
Size: 297 851 99 199
Fragments arranged by size:
851 297 199 99
TatI w'GTAC_w
Cuts at: 0 228 868 1446
Size: 228 640 578
Fragments arranged by size:
640 578 228
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1227 1446
Size: 1227 219
Enzymes that do cut:
AcuI AflIII ApoI AseI BglII Bme1580I BmgBI BmrI
BmtI Bpu10I BpuEI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BstYI ClaI DraI EaeI EarI Eco57MI
EcoO109I FalI Hin4I MfeI MslI MspA1I NdeI NheI
NspI PasI PciI PflMI PpiI PpuMI SanDI SmlI
SphI SspI StuI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI
BplI BpmI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MluI MmeI
MscI NaeI NarI NcoI NgoMIV NotI NruI NsiI
PacI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI XmnI ZraI