Restriction map for
pF1KB8702
(Linear) (Six-base) MAPSORT of: KIBB8702.seq Check: 9633 from: 1 to: 1113
KIBB8702.orf
With 182 enzymes: SgfI *
February 20, 2009 16:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1011 1113
Size: 1011 102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 1113
Size: 118 995
AhdI GACnn_n'nnGTC
Cuts at: 0 1108 1113
Size: 1108 5
AlwNI CAG_nnn'CTG
Cuts at: 0 13 321 576 627 663 882 1113
Size: 13 308 255 51 36 219 231
Fragments arranged by size:
308 255 231 219 51 36 13
ApaI G_GGCC'C
Cuts at: 0 251 701 1113
Size: 251 450 412
Fragments arranged by size:
450 412 251
AvaI C'yCGr_G
Cuts at: 0 42 49 250 397 436 694 700 943
Size: 42 7 201 147 39 258 6 243
Cuts at: 943 1113
Size: 170
Fragments arranged by size:
258 243 201 170 147 42 39 7
6
BanI G'GyrC_C
Cuts at: 0 34 203 376 595 684 756 778 1113
Size: 34 169 173 219 89 72 22 335
Fragments arranged by size:
335 219 173 169 89 72 34 22
BanII G_rGCy'C
Cuts at: 0 251 701 953 1113
Size: 251 450 252 160
Fragments arranged by size:
450 252 251 160
BbeI G_GCGC'C
Cuts at: 0 38 380 599 760 782 1113
Size: 38 342 219 161 22 331
Fragments arranged by size:
342 331 219 161 38 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 600 1113
Size: 600 513
BglI GCCn_nnn'nGGC
Cuts at: 0 26 721 1113
Size: 26 695 392
Fragments arranged by size:
695 392 26
BlpI GC'TnA_GC
Cuts at: 0 1049 1113
Size: 1049 64
Bme1580I G_kGCm'C
Cuts at: 0 251 701 1113
Size: 251 450 412
Fragments arranged by size:
450 412 251
BmgBI CAC'GTC
Cuts at: 0 792 1113
Size: 792 321
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 579 909 1113
Size: 579 330 204
Fragments arranged by size:
579 330 204
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 904 949 1113
Size: 496 408 45 164
Fragments arranged by size:
496 408 164 45
BsaHI Gr'CG_yC
Cuts at: 0 35 377 596 709 757 779 1113
Size: 35 342 219 113 48 22 334
Fragments arranged by size:
342 334 219 113 48 35 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 823 961 1113
Size: 823 138 152
Fragments arranged by size:
823 152 138
BseYI C'CCAG_C
Cuts at: 0 156 402 506 521 689 1113
Size: 156 246 104 15 168 424
Fragments arranged by size:
424 246 168 156 104 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 676 1113
Size: 376 300 437
Fragments arranged by size:
437 376 300
BsiEI CG_ry'CG
Cuts at: 0 416 476 725 1113
Size: 416 60 249 388
Fragments arranged by size:
416 388 249 60
BsiHKAI G_wGCw'C
Cuts at: 0 641 872 953 1113
Size: 641 231 81 160
Fragments arranged by size:
641 231 160 81
Bsp1286I G_dGCh'C
Cuts at: 0 251 641 701 872 953 1113
Size: 251 390 60 171 81 160
Fragments arranged by size:
390 251 171 160 81 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1011 1113
Size: 1011 102
BsrFI r'CCGG_y
Cuts at: 0 37 77 136 411 471 484 592 681
Size: 37 40 59 275 60 13 108 89
Cuts at: 681 720 1113
Size: 39 393
Fragments arranged by size:
393 275 108 89 60 59 40 39
37 13
BssHII G'CGCG_C
Cuts at: 0 171 220 310 498 580 1113
Size: 171 49 90 188 82 533
Fragments arranged by size:
533 188 171 90 82 49
BstAPI GCAn_nnn'nTGC
Cuts at: 0 13 663 1113
Size: 13 650 450
Fragments arranged by size:
650 450 13
BstXI CCAn_nnnn'nTGG
Cuts at: 0 372 1113
Size: 372 741
Bsu36I CC'TnA_GG
Cuts at: 0 1098 1113
Size: 1098 15
BtgI C'CryG_G
Cuts at: 0 243 380 915 1113
Size: 243 137 535 198
Fragments arranged by size:
535 243 198 137
BtsI GCAGTG_nn'
Cuts at: 0 661 1113
Size: 661 452
DraIII CAC_nnn'GTG
Cuts at: 0 654 1113
Size: 654 459
DrdI GACnn_nn'nnGTC
Cuts at: 0 796 1113
Size: 796 317
EaeI y'GGCC_r
Cuts at: 0 413 443 473 533 722 933 1033 1113
Size: 413 30 30 60 189 211 100 80
Fragments arranged by size:
413 211 189 100 80 60 30 30
EagI C'GGCC_G
Cuts at: 0 413 473 722 1113
Size: 413 60 249 391
Fragments arranged by size:
413 391 249 60
EarI CTCTTCn'nnn_
Cuts at: 0 244 292 1113
Size: 244 48 821
Fragments arranged by size:
821 244 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 145 1113
Size: 145 968
EcoICRI GAG'CTC
Cuts at: 0 951 1113
Size: 951 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 496 904 949 1113
Size: 118 378 408 45 164
Fragments arranged by size:
408 378 164 118 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 271 295 1113
Size: 271 24 818
Fragments arranged by size:
818 271 24
EcoO109I rG'GnC_Cy
Cuts at: 0 46 1113
Size: 46 1067
HaeII r_GCGC'y
Cuts at: 0 38 238 262 321 371 380 550 599
Size: 38 200 24 59 50 9 170 49
Cuts at: 599 627 760 782 1113
Size: 28 133 22 331
Fragments arranged by size:
331 200 170 133 59 50 49 38
28 24 22 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1094 1113
Size: 1094 19
HincII GTy'rAC
Cuts at: 0 1043 1113
Size: 1043 70
KasI G'GCGC_C
Cuts at: 0 34 376 595 756 778 1113
Size: 34 342 219 161 22 335
Fragments arranged by size:
342 335 219 161 34 22
MscI TGG'CCA
Cuts at: 0 1035 1113
Size: 1035 78
MslI CAynn'nnrTG
Cuts at: 0 616 1113
Size: 616 497
MspA1I CmG'CkG
Cuts at: 0 10 156 210 351 382 516 525 573
Size: 10 146 54 141 31 134 9 48
Cuts at: 573 803 932 1113
Size: 230 129 181
Fragments arranged by size:
230 181 146 141 134 129 54 48
31 10 9
NaeI GCC'GGC
Cuts at: 0 39 138 413 473 683 1113
Size: 39 99 275 60 210 430
Fragments arranged by size:
430 275 210 99 60 39
NarI GG'CG_CC
Cuts at: 0 35 377 596 757 779 1113
Size: 35 342 219 161 22 334
Fragments arranged by size:
342 334 219 161 35 22
NgoMIV G'CCGG_C
Cuts at: 0 37 136 411 471 681 1113
Size: 37 99 275 60 210 432
Fragments arranged by size:
432 275 210 99 60 37
NspI r_CATG'y
Cuts at: 0 621 1113
Size: 621 492
PasI CC'CwG_GG
Cuts at: 0 372 774 1113
Size: 372 402 339
Fragments arranged by size:
402 372 339
PflMI CCAn_nnn'nTGG
Cuts at: 0 321 576 882 972 1113
Size: 321 255 306 90 141
Fragments arranged by size:
321 306 255 141 90
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 733 765 1113
Size: 733 32 348
Fragments arranged by size:
733 348 32
PpuMI rG'GwC_Cy
Cuts at: 0 46 1113
Size: 46 1067
PspOMI G'GGCC_C
Cuts at: 0 247 697 1113
Size: 247 450 416
Fragments arranged by size:
450 416 247
PspXI vC'TCGA_Gb
Cuts at: 0 397 436 1113
Size: 397 39 677
Fragments arranged by size:
677 397 39
PstI C_TGCA'G
Cuts at: 0 18 356 545 1022 1113
Size: 18 338 189 477 91
Fragments arranged by size:
477 338 189 91 18
PvuII CAG'CTG
Cuts at: 0 156 210 516 525 573 1113
Size: 156 54 306 9 48 540
Fragments arranged by size:
540 306 156 54 48 9
SacI G_AGCT'C
Cuts at: 0 953 1113
Size: 953 160
SacII CC_GC'GG
Cuts at: 0 383 1113
Size: 383 730
SanDI GG'GwC_CC
Cuts at: 0 46 1113
Size: 46 1067
SapI GCTCTTCn'nnn_
Cuts at: 0 244 1113
Size: 244 869
ScaI AGT'ACT
Cuts at: 0 892 1113
Size: 892 221
SfcI C'TryA_G
Cuts at: 0 14 352 541 1018 1113
Size: 14 338 189 477 95
Fragments arranged by size:
477 338 189 95 14
SfoI GGC'GCC
Cuts at: 0 36 378 597 758 780 1113
Size: 36 342 219 161 22 333
Fragments arranged by size:
342 333 219 161 36 22
SgrAI Cr'CCGG_yG
Cuts at: 0 592 1113
Size: 592 521
SmaI CCC'GGG
Cuts at: 0 44 252 696 702 1113
Size: 44 208 444 6 411
Fragments arranged by size:
444 411 208 44 6
SmlI C'TyrA_G
Cuts at: 0 397 436 1113
Size: 397 39 677
Fragments arranged by size:
677 397 39
SphI G_CATG'C
Cuts at: 0 621 1113
Size: 621 492
SrfI GCCC'GGGC
Cuts at: 0 696 1113
Size: 696 417
StuI AGG'CCT
Cuts at: 0 20 1113
Size: 20 1093
TatI w'GTAC_w
Cuts at: 0 890 1113
Size: 890 223
XhoI C'TCGA_G
Cuts at: 0 397 436 1113
Size: 397 39 677
Fragments arranged by size:
677 397 39
XmaI C'CCGG_G
Cuts at: 0 42 250 694 700 1113
Size: 42 208 444 6 413
Fragments arranged by size:
444 413 208 42 6
XmnI GAAnn'nnTTC
Cuts at: 0 106 1113
Size: 106 1007
Enzymes that do cut:
AarI AcuI AhdI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BglI BlpI Bme1580I BmgBI BplI BpmI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrFI BssHII BstAPI BstXI Bsu36I BtgI BtsI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII KasI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PflMI PpiI PpuMI
PspOMI PspXI PstI PvuII SacI SacII SanDI SapI
ScaI SfcI SfoI SgrAI SmaI SmlI SphI SrfI
StuI TatI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BmrI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssSI BstBI BstEII BstYI BstZ17I BtgZI
ClaI CspCI DraI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MmeI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PshAI PsiI PsrI PvuI RsrII SalI
SbfI SexAI SfiI SgfI SnaBI SpeI SspI StyI
SwaI TaqII TstI Tth111I XbaI XcmI ZraI