(Linear) (Six-base) MAPSORT of: KIBB8674.seq  Check: 7522  from: 1  to: 1575



KIBB8674.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1161    1575 
   Size:       1161     414
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     566     895    1575 
   Size:        566     329     680

  Fragments arranged by size: 

                 680     566     329
 
AflIII A'CryG_T

Cuts at:      0     177    1575 
   Size:        177    1398
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1465    1497    1575 
   Size:       1465      32      78

  Fragments arranged by size: 

                1465      78      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     460     859    1414    1575 
   Size:        460     399     555     161

  Fragments arranged by size: 

                 555     460     399     161
 
ApoI r'AATT_y

Cuts at:      0     355     587    1305    1575 
   Size:        355     232     718     270

  Fragments arranged by size: 

                 718     355     270     232
 
AvrII C'CTAG_G

Cuts at:      0    1257    1575 
   Size:       1257     318
 
BanII G_rGCy'C

Cuts at:      0     900    1575 
   Size:        900     675
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     509    1575 
   Size:        509    1066
 
BglII A'GATC_T

Cuts at:      0    1285    1575 
   Size:       1285     290
 
BmgBI CAC'GTC

Cuts at:      0      36    1575 
   Size:         36    1539
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     416    1121    1575 
   Size:        416     705     454

  Fragments arranged by size: 

                 705     454     416
 
Bpu10I CC'TnA_GC

Cuts at:      0     860    1575 
   Size:        860     715
 
BsaAI yAC'GTr

Cuts at:      0     280    1575 
   Size:        280    1295
 
BsaWI w'CCGG_w

Cuts at:      0    1509    1575 
   Size:       1509      66
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     414     444    1575 
   Size:        414      30    1131

  Fragments arranged by size: 

                1131     414      30
 
BsmI GAATG_Cn'

Cuts at:      0    1029    1223    1575 
   Size:       1029     194     352

  Fragments arranged by size: 

                1029     352     194
 
Bsp1286I G_dGCh'C

Cuts at:      0     900    1575 
   Size:        900     675
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     427     894    1161    1575 
   Size:        427     467     267     414

  Fragments arranged by size: 

                 467     427     414     267
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1327    1575 
   Size:       1327     248
 
BstYI r'GATC_y

Cuts at:      0     921    1285    1575 
   Size:        921     364     290

  Fragments arranged by size: 

                 921     364     290
 
DraI TTT'AAA

Cuts at:      0     669     779    1575 
   Size:        669     110     796

  Fragments arranged by size: 

                 796     669     110
 
DraIII CAC_nnn'GTG

Cuts at:      0    1201    1575 
   Size:       1201     374
 
EarI CTCTTCn'nnn_

Cuts at:      0     206    1575 
   Size:        206    1369
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     416     566     895    1121    1575 
   Size:        416     150     329     226     454

  Fragments arranged by size: 

                 454     416     329     226     150
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     434    1575 
   Size:        434    1141
 
EcoO109I rG'GnC_Cy

Cuts at:      0     366    1261    1575 
   Size:        366     895     314

  Fragments arranged by size: 

                 895     366     314
 
EcoRI G'AATT_C

Cuts at:      0    1305    1575 
   Size:       1305     270
 
EcoRV GAT'ATC

Cuts at:      0     386     972    1575 
   Size:        386     586     603

  Fragments arranged by size: 

                 603     586     386
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     906     938    1447    1479    1575 
   Size:        906      32     509      32      96

  Fragments arranged by size: 

                 906     509      96      32      32
 
MfeI C'AATT_G

Cuts at:      0     711    1575 
   Size:        711     864
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     949    1575 
   Size:         87     862     626

  Fragments arranged by size: 

                 862     626      87
 
MspA1I CmG'CkG

Cuts at:      0     457    1575 
   Size:        457    1118
 
NdeI CA'TA_TG

Cuts at:      0      94    1575 
   Size:         94    1481
 
NspI r_CATG'y

Cuts at:      0     181    1575 
   Size:        181    1394
 
PciI A'CATG_T

Cuts at:      0     177    1575 
   Size:        177    1398
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     716     984    1575 
   Size:        716     268     591

  Fragments arranged by size: 

                 716     591     268
 
PfoI T'CCnGG_A

Cuts at:      0    1061    1575 
   Size:       1061     514
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1378    1410    1575 
   Size:       1378      32     165

  Fragments arranged by size: 

                1378     165      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     366    1261    1575 
   Size:        366     895     314

  Fragments arranged by size: 

                 895     366     314
 
PstI C_TGCA'G

Cuts at:      0     905    1575 
   Size:        905     670
 
PvuII CAG'CTG

Cuts at:      0     457    1575 
   Size:        457    1118
 
SbfI CC_TGCA'GG

Cuts at:      0     905    1575 
   Size:        905     670
 
ScaI AGT'ACT

Cuts at:      0     114    1575 
   Size:        114    1461
 
SfcI C'TryA_G

Cuts at:      0      65     901    1251    1402    1575 
   Size:         65     836     350     151     173

  Fragments arranged by size: 

                 836     350     173     151      65
 
StyI C'CwwG_G

Cuts at:      0    1257    1575 
   Size:       1257     318
 
TatI w'GTAC_w

Cuts at:      0      56     112    1109    1575 
   Size:         56      56     997     466

  Fragments arranged by size: 

                 997     466      56      56
 
XmnI GAAnn'nnTTC

Cuts at:      0     356    1575 
   Size:        356    1219
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AloI    AlwNI     ApoI    AvrII    BanII
     BbsI    BglII    BmgBI     BpmI   Bpu10I    BsaAI    BsaWI    BsaXI
     BsmI Bsp1286I    BspMI   BstAPI    BstYI     DraI   DraIII     EarI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV    Hin4I     MfeI     MmeI
   MspA1I     NdeI     NspI     PciI    PflMI     PfoI     PpiI    PpuMI
     PstI    PvuII     SbfI     ScaI     SfcI     StyI     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I     BmrI     BmtI     BplI    BpuEI
     BsaI    BsaBI    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DrdI     EaeI     EagI     EciI
  EcoICRI     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MluI     MscI     MslI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI