(Linear) (Six-base) MAPSORT of: KIBB8657.seq Check: 2430 from: 1 to: 1362 KIBB8657.orf With 182 enzymes: SgfI * March 3, 2008 11:07 .. Acc65I G'GTAC_C Cuts at: 0 1055 1362 Size: 1055 307 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 750 1362 Size: 750 612 AflIII A'CryG_T Cuts at: 0 1064 1362 Size: 1064 298 AleI CACnn'nnGTG Cuts at: 0 76 1362 Size: 76 1286 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 766 798 1362 Size: 766 32 564 Fragments arranged by size: 766 564 32 AlwNI CAG_nnn'CTG Cuts at: 0 384 1362 Size: 384 978 ApaLI G'TGCA_C Cuts at: 0 814 1362 Size: 814 548 ApoI r'AATT_y Cuts at: 0 160 1362 Size: 160 1202 AseI AT'TA_AT Cuts at: 0 569 1362 Size: 569 793 AvaI C'yCGr_G Cuts at: 0 829 1362 Size: 829 533 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1211 1244 1362 Size: 1211 33 118 Fragments arranged by size: 1211 118 33 BanI G'GyrC_C Cuts at: 0 31 1042 1055 1282 1362 Size: 31 1011 13 227 80 Fragments arranged by size: 1011 227 80 31 13 BanII G_rGCy'C Cuts at: 0 863 1362 Size: 863 499 BbeI G_GCGC'C Cuts at: 0 35 1286 1362 Size: 35 1251 76 Fragments arranged by size: 1251 76 35 BbsI GAAGACnn'nnnn_ Cuts at: 0 549 1362 Size: 549 813 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 939 973 1252 1286 1362 Size: 939 34 279 34 76 Fragments arranged by size: 939 279 76 34 34 BglII A'GATC_T Cuts at: 0 197 326 1151 1362 Size: 197 129 825 211 Fragments arranged by size: 825 211 197 129 Bme1580I G_kGCm'C Cuts at: 0 818 1104 1362 Size: 818 286 258 Fragments arranged by size: 818 286 258 BmtI G_CTAG'C Cuts at: 0 1009 1362 Size: 1009 353 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 234 418 780 1312 1362 Size: 234 184 362 532 50 Fragments arranged by size: 532 362 234 184 50 BsaI GGTCTCn'nnnn_ Cuts at: 0 802 1362 Size: 802 560 BsaAI yAC'GTr Cuts at: 0 1227 1362 Size: 1227 135 BsaHI Gr'CG_yC Cuts at: 0 32 460 1283 1362 Size: 32 428 823 79 Fragments arranged by size: 823 428 79 32 BsaWI w'CCGG_w Cuts at: 0 151 1362 Size: 151 1211 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 328 358 394 424 1362 Size: 328 30 36 30 938 Fragments arranged by size: 938 328 36 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 415 743 871 1362 Size: 415 328 128 491 Fragments arranged by size: 491 415 328 128 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 571 1362 Size: 571 791 BsiHKAI G_wGCw'C Cuts at: 0 818 896 1337 1362 Size: 818 78 441 25 Fragments arranged by size: 818 441 78 25 BsmI GAATG_Cn' Cuts at: 0 211 1362 Size: 211 1151 BsmBI CGTCTCn'nnnn_ Cuts at: 0 451 1362 Size: 451 911 Bsp1286I G_dGCh'C Cuts at: 0 818 863 896 1104 1337 1362 Size: 818 45 33 208 233 25 Fragments arranged by size: 818 233 208 45 33 25 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 543 923 1362 Size: 543 380 439 Fragments arranged by size: 543 439 380 BsrBI CCG'CTC Cuts at: 0 20 1362 Size: 20 1342 BsrFI r'CCGG_y Cuts at: 0 28 34 1118 1362 Size: 28 6 1084 244 Fragments arranged by size: 1084 244 28 6 BsrGI T'GTAC_A Cuts at: 0 410 1061 1067 1362 Size: 410 651 6 295 Fragments arranged by size: 651 410 295 6 BstYI r'GATC_y Cuts at: 0 197 326 1022 1151 1362 Size: 197 129 696 129 211 Fragments arranged by size: 696 211 197 129 129 BtgI C'CryG_G Cuts at: 0 653 962 1027 1362 Size: 653 309 65 335 Fragments arranged by size: 653 335 309 65 EaeI y'GGCC_r Cuts at: 0 36 108 263 984 1362 Size: 36 72 155 721 378 Fragments arranged by size: 721 378 155 72 36 EarI CTCTTCn'nnn_ Cuts at: 0 52 1362 Size: 52 1310 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 780 958 1362 Size: 780 178 404 Fragments arranged by size: 780 404 178 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 234 418 750 780 1312 1362 Size: 234 184 332 30 532 50 Fragments arranged by size: 532 332 234 184 50 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 1321 1362 Size: 1321 41 HaeII r_GCGC'y Cuts at: 0 35 1286 1310 1362 Size: 35 1251 24 52 Fragments arranged by size: 1251 52 35 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 582 614 695 727 1150 1182 1362 Size: 582 32 81 32 423 32 180 Fragments arranged by size: 582 423 180 81 32 32 32 KasI G'GCGC_C Cuts at: 0 31 1282 1362 Size: 31 1251 80 Fragments arranged by size: 1251 80 31 KpnI G_GTAC'C Cuts at: 0 1059 1362 Size: 1059 303 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 371 1362 Size: 371 991 MscI TGG'CCA Cuts at: 0 265 1362 Size: 265 1097 MslI CAynn'nnrTG Cuts at: 0 76 436 1362 Size: 76 360 926 Fragments arranged by size: 926 360 76 MspA1I CmG'CkG Cuts at: 0 42 100 1029 1207 1362 Size: 42 58 929 178 155 Fragments arranged by size: 929 178 155 58 42 NaeI GCC'GGC Cuts at: 0 30 36 1362 Size: 30 6 1326 Fragments arranged by size: 1326 30 6 NarI GG'CG_CC Cuts at: 0 32 1283 1362 Size: 32 1251 79 Fragments arranged by size: 1251 79 32 NgoMIV G'CCGG_C Cuts at: 0 28 34 1362 Size: 28 6 1328 Fragments arranged by size: 1328 28 6 NheI G'CTAG_C Cuts at: 0 1005 1362 Size: 1005 357 NspI r_CATG'y Cuts at: 0 783 1068 1362 Size: 783 285 294 Fragments arranged by size: 783 294 285 PasI CC'CwG_GG Cuts at: 0 526 1362 Size: 526 836 PciI A'CATG_T Cuts at: 0 1064 1362 Size: 1064 298 PflMI CCAn_nnn'nTGG Cuts at: 0 869 1362 Size: 869 493 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 229 261 1362 Size: 229 32 1101 Fragments arranged by size: 1101 229 32 PshAI GACnn'nnGTC Cuts at: 0 1130 1362 Size: 1130 232 PsiI TTA'TAA Cuts at: 0 158 1362 Size: 158 1204 PstI C_TGCA'G Cuts at: 0 477 1212 1362 Size: 477 735 150 Fragments arranged by size: 735 477 150 PvuII CAG'CTG Cuts at: 0 42 100 1207 1362 Size: 42 58 1107 155 Fragments arranged by size: 1107 155 58 42 RsrII CG'GwC_CG Cuts at: 0 966 1362 Size: 966 396 SacII CC_GC'GG Cuts at: 0 1030 1362 Size: 1030 332 SapI GCTCTTCn'nnn_ Cuts at: 0 52 1362 Size: 52 1310 SbfI CC_TGCA'GG Cuts at: 0 477 1362 Size: 477 885 SfcI C'TryA_G Cuts at: 0 336 473 1208 1362 Size: 336 137 735 154 Fragments arranged by size: 735 336 154 137 SfoI GGC'GCC Cuts at: 0 33 1284 1362 Size: 33 1251 78 Fragments arranged by size: 1251 78 33 SmlI C'TyrA_G Cuts at: 0 829 1362 Size: 829 533 StuI AGG'CCT Cuts at: 0 479 1362 Size: 479 883 StyI C'CwwG_G Cuts at: 0 286 1362 Size: 286 1076 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1030 1258 1267 1362 Size: 1030 228 9 95 Fragments arranged by size: 1030 228 95 9 TatI w'GTAC_w Cuts at: 0 410 1061 1067 1362 Size: 410 651 6 295 Fragments arranged by size: 651 410 295 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 211 243 1362 Size: 211 32 1119 Fragments arranged by size: 1119 211 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 258 354 1152 1362 Size: 258 96 798 210 Fragments arranged by size: 798 258 210 96 XhoI C'TCGA_G Cuts at: 0 829 1362 Size: 829 533 Enzymes that do cut: Acc65I AcuI AflIII AleI AloI AlwNI ApaLI ApoI AseI AvaI BaeI BanI BanII BbeI BbsI BcgI BglII Bme1580I BmtI BpmI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BstYI BtgI EaeI EarI EciI Eco57MI EcoNI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI PasI PciI PflMI PpiI PshAI PsiI PstI PvuII RsrII SacII SapI SbfI SfcI SfoI SmlI StuI StyI TaqII TatI TstI XcmI XhoI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AhdI AlfI ApaI AscI AsiSI AvrII BamHI BbvCI BciVI BclI BfrBI BglI BlpI BmgBI BmrI BplI Bpu10I BpuEI BsaBI BseYI BsiEI BsiWI BspEI BspHI BsrDI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI NcoI NdeI NotI NruI NsiI PacI PfoI PmeI PmlI PpuMI PspOMI PspXI PsrI PvuI SacI SalI SanDI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XmaI XmnI ZraI