Restriction map for pF1KB8657
 (Linear) (Six-base) MAPSORT of: KIBB8657.seq  Check: 2430  from: 1  to: 1362



KIBB8657.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

Acc65I G'GTAC_C

Cuts at:      0    1055    1362 
   Size:       1055     307
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     750    1362 
   Size:        750     612
 
AflIII A'CryG_T

Cuts at:      0    1064    1362 
   Size:       1064     298
 
AleI CACnn'nnGTG

Cuts at:      0      76    1362 
   Size:         76    1286
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     766     798    1362 
   Size:        766      32     564

  Fragments arranged by size: 

                 766     564      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     384    1362 
   Size:        384     978
 
ApaLI G'TGCA_C

Cuts at:      0     814    1362 
   Size:        814     548
 
ApoI r'AATT_y

Cuts at:      0     160    1362 
   Size:        160    1202
 
AseI AT'TA_AT

Cuts at:      0     569    1362 
   Size:        569     793
 
AvaI C'yCGr_G

Cuts at:      0     829    1362 
   Size:        829     533
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1211    1244    1362 
   Size:       1211      33     118

  Fragments arranged by size: 

                1211     118      33
 
BanI G'GyrC_C

Cuts at:      0      31    1042    1055    1282    1362 
   Size:         31    1011      13     227      80

  Fragments arranged by size: 

                1011     227      80      31      13
 
BanII G_rGCy'C

Cuts at:      0     863    1362 
   Size:        863     499
 
BbeI G_GCGC'C

Cuts at:      0      35    1286    1362 
   Size:         35    1251      76

  Fragments arranged by size: 

                1251      76      35
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     549    1362 
   Size:        549     813
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     939     973    1252    1286    1362 
   Size:        939      34     279      34      76

  Fragments arranged by size: 

                 939     279      76      34      34
 
BglII A'GATC_T

Cuts at:      0     197     326    1151    1362 
   Size:        197     129     825     211

  Fragments arranged by size: 

                 825     211     197     129
 
Bme1580I G_kGCm'C

Cuts at:      0     818    1104    1362 
   Size:        818     286     258

  Fragments arranged by size: 

                 818     286     258
 
BmtI G_CTAG'C

Cuts at:      0    1009    1362 
   Size:       1009     353
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     234     418     780    1312    1362 
   Size:        234     184     362     532      50

  Fragments arranged by size: 

                 532     362     234     184      50
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     802    1362 
   Size:        802     560
 
BsaAI yAC'GTr

Cuts at:      0    1227    1362 
   Size:       1227     135
 
BsaHI Gr'CG_yC

Cuts at:      0      32     460    1283    1362 
   Size:         32     428     823      79

  Fragments arranged by size: 

                 823     428      79      32
 
BsaWI w'CCGG_w

Cuts at:      0     151    1362 
   Size:        151    1211
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     328     358     394     424    1362 
   Size:        328      30      36      30     938

  Fragments arranged by size: 

                 938     328      36      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     415     743     871    1362 
   Size:        415     328     128     491

  Fragments arranged by size: 

                 491     415     328     128
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571    1362 
   Size:        571     791
 
BsiHKAI G_wGCw'C

Cuts at:      0     818     896    1337    1362 
   Size:        818      78     441      25

  Fragments arranged by size: 

                 818     441      78      25
 
BsmI GAATG_Cn'

Cuts at:      0     211    1362 
   Size:        211    1151
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     451    1362 
   Size:        451     911
 
Bsp1286I G_dGCh'C

Cuts at:      0     818     863     896    1104    1337    1362 
   Size:        818      45      33     208     233      25

  Fragments arranged by size: 

                 818     233     208      45      33      25
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     543     923    1362 
   Size:        543     380     439

  Fragments arranged by size: 

                 543     439     380
 
BsrBI CCG'CTC

Cuts at:      0      20    1362 
   Size:         20    1342
 
BsrFI r'CCGG_y

Cuts at:      0      28      34    1118    1362 
   Size:         28       6    1084     244

  Fragments arranged by size: 

                1084     244      28       6
 
BsrGI T'GTAC_A

Cuts at:      0     410    1061    1067    1362 
   Size:        410     651       6     295

  Fragments arranged by size: 

                 651     410     295       6
 
BstYI r'GATC_y

Cuts at:      0     197     326    1022    1151    1362 
   Size:        197     129     696     129     211

  Fragments arranged by size: 

                 696     211     197     129     129
 
BtgI C'CryG_G

Cuts at:      0     653     962    1027    1362 
   Size:        653     309      65     335

  Fragments arranged by size: 

                 653     335     309      65
 
EaeI y'GGCC_r

Cuts at:      0      36     108     263     984    1362 
   Size:         36      72     155     721     378

  Fragments arranged by size: 

                 721     378     155      72      36
 
EarI CTCTTCn'nnn_

Cuts at:      0      52    1362 
   Size:         52    1310
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     780     958    1362 
   Size:        780     178     404

  Fragments arranged by size: 

                 780     404     178
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     234     418     750     780    1312    1362 
   Size:        234     184     332      30     532      50

  Fragments arranged by size: 

                 532     332     234     184      50      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1321    1362 
   Size:       1321      41
 
HaeII r_GCGC'y

Cuts at:      0      35    1286    1310    1362 
   Size:         35    1251      24      52

  Fragments arranged by size: 

                1251      52      35      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     582     614     695     727    1150    1182    1362 
   Size:        582      32      81      32     423      32     180

  Fragments arranged by size: 

                 582     423     180      81      32      32      32
 
KasI G'GCGC_C

Cuts at:      0      31    1282    1362 
   Size:         31    1251      80

  Fragments arranged by size: 

                1251      80      31
 
KpnI G_GTAC'C

Cuts at:      0    1059    1362 
   Size:       1059     303
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     371    1362 
   Size:        371     991
 
MscI TGG'CCA

Cuts at:      0     265    1362 
   Size:        265    1097
 
MslI CAynn'nnrTG

Cuts at:      0      76     436    1362 
   Size:         76     360     926

  Fragments arranged by size: 

                 926     360      76
 
MspA1I CmG'CkG

Cuts at:      0      42     100    1029    1207    1362 
   Size:         42      58     929     178     155

  Fragments arranged by size: 

                 929     178     155      58      42
 
NaeI GCC'GGC

Cuts at:      0      30      36    1362 
   Size:         30       6    1326

  Fragments arranged by size: 

                1326      30       6
 
NarI GG'CG_CC

Cuts at:      0      32    1283    1362 
   Size:         32    1251      79

  Fragments arranged by size: 

                1251      79      32
 
NgoMIV G'CCGG_C

Cuts at:      0      28      34    1362 
   Size:         28       6    1328

  Fragments arranged by size: 

                1328      28       6
 
NheI G'CTAG_C

Cuts at:      0    1005    1362 
   Size:       1005     357
 
NspI r_CATG'y

Cuts at:      0     783    1068    1362 
   Size:        783     285     294

  Fragments arranged by size: 

                 783     294     285
 
PasI CC'CwG_GG

Cuts at:      0     526    1362 
   Size:        526     836
 
PciI A'CATG_T

Cuts at:      0    1064    1362 
   Size:       1064     298
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     869    1362 
   Size:        869     493
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     229     261    1362 
   Size:        229      32    1101

  Fragments arranged by size: 

                1101     229      32
 
PshAI GACnn'nnGTC

Cuts at:      0    1130    1362 
   Size:       1130     232
 
PsiI TTA'TAA

Cuts at:      0     158    1362 
   Size:        158    1204
 
PstI C_TGCA'G

Cuts at:      0     477    1212    1362 
   Size:        477     735     150

  Fragments arranged by size: 

                 735     477     150
 
PvuII CAG'CTG

Cuts at:      0      42     100    1207    1362 
   Size:         42      58    1107     155

  Fragments arranged by size: 

                1107     155      58      42
 
RsrII CG'GwC_CG

Cuts at:      0     966    1362 
   Size:        966     396
 
SacII CC_GC'GG

Cuts at:      0    1030    1362 
   Size:       1030     332
 
SapI GCTCTTCn'nnn_

Cuts at:      0      52    1362 
   Size:         52    1310
 
SbfI CC_TGCA'GG

Cuts at:      0     477    1362 
   Size:        477     885
 
SfcI C'TryA_G

Cuts at:      0     336     473    1208    1362 
   Size:        336     137     735     154

  Fragments arranged by size: 

                 735     336     154     137
 
SfoI GGC'GCC

Cuts at:      0      33    1284    1362 
   Size:         33    1251      78

  Fragments arranged by size: 

                1251      78      33
 
SmlI C'TyrA_G

Cuts at:      0     829    1362 
   Size:        829     533
 
StuI AGG'CCT

Cuts at:      0     479    1362 
   Size:        479     883
 
StyI C'CwwG_G

Cuts at:      0     286    1362 
   Size:        286    1076
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1030    1258    1267    1362 
   Size:       1030     228       9      95

  Fragments arranged by size: 

                1030     228      95       9
 
TatI w'GTAC_w

Cuts at:      0     410    1061    1067    1362 
   Size:        410     651       6     295

  Fragments arranged by size: 

                 651     410     295       6
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     211     243    1362 
   Size:        211      32    1119

  Fragments arranged by size: 

                1119     211      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     258     354    1152    1362 
   Size:        258      96     798     210

  Fragments arranged by size: 

                 798     258     210      96
 
XhoI C'TCGA_G

Cuts at:      0     829    1362 
   Size:        829     533
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AleI     AloI    AlwNI    ApaLI     ApoI
     AseI     AvaI     BaeI     BanI    BanII     BbeI     BbsI     BcgI
    BglII Bme1580I     BmtI     BpmI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrFI    BsrGI    BstYI     BtgI     EaeI     EarI     EciI
  Eco57MI    EcoNI    HaeII    Hin4I     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NheI     NspI     PasI
     PciI    PflMI     PpiI    PshAI     PsiI     PstI    PvuII    RsrII
    SacII     SapI     SbfI     SfcI     SfoI     SmlI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     ApaI     AscI    AsiSI    AvrII    BamHI    BbvCI    BciVI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BplI   Bpu10I
    BpuEI    BsaBI    BseYI    BsiEI    BsiWI    BspEI    BspHI    BsrDI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     MfeI     MluI     NcoI     NdeI     NotI
     NruI     NsiI     PacI     PfoI     PmeI     PmlI    PpuMI   PspOMI
    PspXI     PsrI     PvuI     SacI     SalI    SanDI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI  Tth111I     XbaI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com