Restriction map for
pF1KB8655
(Linear) (Six-base) MAPSORT of: KIBB8655.seq Check: 7548 from: 1 to: 1698
KIBB8655.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 960 1424 1698
Size: 960 464 274
Fragments arranged by size:
960 464 274
AccI GT'mk_AC
Cuts at: 0 242 326 1698
Size: 242 84 1372
Fragments arranged by size:
1372 242 84
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 384 985 1698
Size: 91 293 601 713
Fragments arranged by size:
713 601 293 91
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1304 1432 1480 1698
Size: 1304 128 48 218
Fragments arranged by size:
1304 218 128 48
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 772 804 1698
Size: 772 32 894
Fragments arranged by size:
894 772 32
AlwNI CAG_nnn'CTG
Cuts at: 0 157 1200 1698
Size: 157 1043 498
Fragments arranged by size:
1043 498 157
ApoI r'AATT_y
Cuts at: 0 1014 1048 1698
Size: 1014 34 650
Fragments arranged by size:
1014 650 34
AvaI C'yCGr_G
Cuts at: 0 1159 1698
Size: 1159 539
BanII G_rGCy'C
Cuts at: 0 1159 1698
Size: 1159 539
BbsI GAAGACnn'nnnn_
Cuts at: 0 278 398 949 1698
Size: 278 120 551 749
Fragments arranged by size:
749 551 278 120
BbvCI CC'TCA_GC
Cuts at: 0 1652 1698
Size: 1652 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1293 1327 1698
Size: 1293 34 371
Fragments arranged by size:
1293 371 34
BlpI GC'TnA_GC
Cuts at: 0 1405 1698
Size: 1405 293
Bme1580I G_kGCm'C
Cuts at: 0 1360 1698
Size: 1360 338
BmgBI CAC'GTC
Cuts at: 0 78 1125 1698
Size: 78 1047 573
Fragments arranged by size:
1047 573 78
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 568 1698
Size: 568 1130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 1197 1346 1698
Size: 916 281 149 352
Fragments arranged by size:
916 352 281 149
Bpu10I CC'TnA_GC
Cuts at: 0 1173 1396 1489 1652 1698
Size: 1173 223 93 163 46
Fragments arranged by size:
1173 223 163 93 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1405 1698
Size: 208 1197 293
Fragments arranged by size:
1197 293 208
BsaI GGTCTCn'nnnn_
Cuts at: 0 1528 1698
Size: 1528 170
BsaAI yAC'GTr
Cuts at: 0 132 714 1698
Size: 132 582 984
Fragments arranged by size:
984 582 132
BsaWI w'CCGG_w
Cuts at: 0 761 1698
Size: 761 937
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 224 254 772 802 1195 1225 1698
Size: 224 30 518 30 393 30 473
Fragments arranged by size:
518 473 393 224 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 427 499 525 790 1698
Size: 427 72 26 265 908
Fragments arranged by size:
908 427 265 72 26
BseYI C'CCAG_C
Cuts at: 0 1277 1698
Size: 1277 421
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 783 1093 1597 1698
Size: 783 310 504 101
Fragments arranged by size:
783 504 310 101
BsiHKAI G_wGCw'C
Cuts at: 0 839 1698
Size: 839 859
BsmI GAATG_Cn'
Cuts at: 0 426 1085 1698
Size: 426 659 613
Fragments arranged by size:
659 613 426
Bsp1286I G_dGCh'C
Cuts at: 0 839 1159 1360 1698
Size: 839 320 201 338
Fragments arranged by size:
839 338 320 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 960 1424 1698
Size: 960 464 274
Fragments arranged by size:
960 464 274
BsrDI GCAATG_nn'
Cuts at: 0 1286 1698
Size: 1286 412
BsrFI r'CCGG_y
Cuts at: 0 1386 1448 1698
Size: 1386 62 250
Fragments arranged by size:
1386 250 62
BstAPI GCAn_nnn'nTGC
Cuts at: 0 504 1698
Size: 504 1194
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1125 1698
Size: 1125 573
BstYI r'GATC_y
Cuts at: 0 101 806 1698
Size: 101 705 892
Fragments arranged by size:
892 705 101
BstZ17I GTA'TAC
Cuts at: 0 327 1698
Size: 327 1371
BtgI C'CryG_G
Cuts at: 0 596 791 992 1239 1514 1698
Size: 596 195 201 247 275 184
Fragments arranged by size:
596 275 247 201 195 184
EaeI y'GGCC_r
Cuts at: 0 1115 1698
Size: 1115 583
EarI CTCTTCn'nnn_
Cuts at: 0 67 404 416 476 537 1028 1698
Size: 67 337 12 60 61 491 670
Fragments arranged by size:
670 491 337 67 61 60 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 817 1698
Size: 817 881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 384 916 985 1197 1346 1698
Size: 91 293 532 69 212 149 352
Fragments arranged by size:
532 352 293 212 149 91 69
EcoNI CCTnn'n_nnAGG
Cuts at: 0 967 1698
Size: 967 731
EcoO109I rG'GnC_Cy
Cuts at: 0 54 633 1327 1698
Size: 54 579 694 371
Fragments arranged by size:
694 579 371 54
HaeII r_GCGC'y
Cuts at: 0 741 1455 1698
Size: 741 714 243
Fragments arranged by size:
741 714 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1195 1227 1698
Size: 1195 32 471
Fragments arranged by size:
1195 471 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 645 1698
Size: 645 1053
MscI TGG'CCA
Cuts at: 0 1117 1698
Size: 1117 581
MslI CAynn'nnrTG
Cuts at: 0 601 703 1069 1698
Size: 601 102 366 629
Fragments arranged by size:
629 601 366 102
MspA1I CmG'CkG
Cuts at: 0 519 1266 1295 1588 1698
Size: 519 747 29 293 110
Fragments arranged by size:
747 519 293 110 29
NaeI GCC'GGC
Cuts at: 0 1388 1698
Size: 1388 310
NcoI C'CATG_G
Cuts at: 0 596 992 1239 1698
Size: 596 396 247 459
Fragments arranged by size:
596 459 396 247
NgoMIV G'CCGG_C
Cuts at: 0 1386 1698
Size: 1386 312
NspI r_CATG'y
Cuts at: 0 648 1068 1698
Size: 648 420 630
Fragments arranged by size:
648 630 420
PasI CC'CwG_GG
Cuts at: 0 1323 1698
Size: 1323 375
PflMI CCAn_nnn'nTGG
Cuts at: 0 157 993 1200 1245 1698
Size: 157 836 207 45 453
Fragments arranged by size:
836 453 207 157 45
PfoI T'CCnGG_A
Cuts at: 0 932 1213 1603 1698
Size: 932 281 390 95
Fragments arranged by size:
932 390 281 95
PmlI CAC'GTG
Cuts at: 0 132 1698
Size: 132 1566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 772 804 1698
Size: 772 32 894
Fragments arranged by size:
894 772 32
PpuMI rG'GwC_Cy
Cuts at: 0 54 633 1698
Size: 54 579 1065
Fragments arranged by size:
1065 579 54
PshAI GACnn'nnGTC
Cuts at: 0 953 1698
Size: 953 745
PstI C_TGCA'G
Cuts at: 0 737 1698
Size: 737 961
PvuII CAG'CTG
Cuts at: 0 1266 1295 1698
Size: 1266 29 403
Fragments arranged by size:
1266 403 29
SapI GCTCTTCn'nnn_
Cuts at: 0 416 1698
Size: 416 1282
SfcI C'TryA_G
Cuts at: 0 733 1698
Size: 733 965
SmlI C'TyrA_G
Cuts at: 0 187 1420 1698
Size: 187 1233 278
Fragments arranged by size:
1233 278 187
StuI AGG'CCT
Cuts at: 0 112 1698
Size: 112 1586
StyI C'CwwG_G
Cuts at: 0 596 629 992 1239 1698
Size: 596 33 363 247 459
Fragments arranged by size:
596 459 363 247 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 149 313 672 1291 1552 1698
Size: 149 164 359 619 261 146
Fragments arranged by size:
619 359 261 164 149 146
TatI w'GTAC_w
Cuts at: 0 1337 1698
Size: 1337 361
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 73 105 1698
Size: 73 32 1593
Fragments arranged by size:
1593 73 32
Tth111I GACn'n_nGTC
Cuts at: 0 588 1126 1698
Size: 588 538 572
Fragments arranged by size:
588 572 538
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 158 1146 1221 1698
Size: 158 988 75 477
Fragments arranged by size:
988 477 158 75
XmnI GAAnn'nnTTC
Cuts at: 0 203 1698
Size: 203 1495
Enzymes that do cut:
AarI AccI AcuI AlfI AloI AlwNI ApoI AvaI
BanII BbsI BbvCI BcgI BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI
BstAPI BstXI BstYI BstZ17I BtgI EaeI EarI EciI
Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI
PmlI PpiI PpuMI PshAI PstI PvuII SapI SfcI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BciVI BclI BfrBI BglI BglII
BmrI BmtI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI XmaI ZraI