Restriction map for
pF1KB8645
(Linear) (Six-base) MAPSORT of: KIBB8645.seq Check: 8566 from: 1 to: 798
KIBB8645.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AccI GT'mk_AC
Cuts at: 0 176 798
Size: 176 622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 388 543 798
Size: 319 69 155 255
Fragments arranged by size:
319 255 155 69
AflIII A'CryG_T
Cuts at: 0 194 798
Size: 194 604
AleI CACnn'nnGTG
Cuts at: 0 532 798
Size: 532 266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 85 798
Size: 85 713
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 529 561 798
Size: 529 32 237
Fragments arranged by size:
529 237 32
AlwNI CAG_nnn'CTG
Cuts at: 0 348 798
Size: 348 450
ApaLI G'TGCA_C
Cuts at: 0 786 798
Size: 786 12
AvaI C'yCGr_G
Cuts at: 0 83 798
Size: 83 715
BanI G'GyrC_C
Cuts at: 0 76 450 798
Size: 76 374 348
Fragments arranged by size:
374 348 76
BanII G_rGCy'C
Cuts at: 0 100 592 798
Size: 100 492 206
Fragments arranged by size:
492 206 100
BbsI GAAGACnn'nnnn_
Cuts at: 0 13 270 798
Size: 13 257 528
Fragments arranged by size:
528 257 13
BciVI GTATCCnnnnn_n'
Cuts at: 0 210 798
Size: 210 588
BglII A'GATC_T
Cuts at: 0 608 798
Size: 608 190
Bme1580I G_kGCm'C
Cuts at: 0 44 455 790 798
Size: 44 411 335 8
Fragments arranged by size:
411 335 44 8
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 798
Size: 370 428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 798
Size: 715 83
BsaI GGTCTCn'nnnn_
Cuts at: 0 696 798
Size: 696 102
BsaAI yAC'GTr
Cuts at: 0 552 798
Size: 552 246
BsaHI Gr'CG_yC
Cuts at: 0 629 677 798
Size: 629 48 121
Fragments arranged by size:
629 121 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 343 373 798
Size: 343 30 425
Fragments arranged by size:
425 343 30
BseYI C'CCAG_C
Cuts at: 0 72 712 798
Size: 72 640 86
Fragments arranged by size:
640 86 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 637 798
Size: 12 625 161
Fragments arranged by size:
625 161 12
BsiHKAI G_wGCw'C
Cuts at: 0 100 557 790 798
Size: 100 457 233 8
Fragments arranged by size:
457 233 100 8
Bsp1286I G_dGCh'C
Cuts at: 0 44 100 455 557 592 790 798
Size: 44 56 355 102 35 198 8
Fragments arranged by size:
355 198 102 56 44 35 8
BssSI C'ACGA_G
Cuts at: 0 580 798
Size: 580 218
BstYI r'GATC_y
Cuts at: 0 344 608 798
Size: 344 264 190
Fragments arranged by size:
344 264 190
BstZ17I GTA'TAC
Cuts at: 0 177 798
Size: 177 621
BtgI C'CryG_G
Cuts at: 0 186 401 429 798
Size: 186 215 28 369
Fragments arranged by size:
369 215 186 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 586 798
Size: 586 212
EaeI y'GGCC_r
Cuts at: 0 123 515 566 798
Size: 123 392 51 232
Fragments arranged by size:
392 232 123 51
EarI CTCTTCn'nnn_
Cuts at: 0 562 798
Size: 562 236
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 406 798
Size: 406 392
EcoICRI GAG'CTC
Cuts at: 0 98 798
Size: 98 700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 370 388 543 798
Size: 319 51 18 155 255
Fragments arranged by size:
319 255 155 51 18
EcoO109I rG'GnC_Cy
Cuts at: 0 644 750 798
Size: 644 106 48
Fragments arranged by size:
644 106 48
FspI TGC'GCA
Cuts at: 0 250 798
Size: 250 548
HaeII r_GCGC'y
Cuts at: 0 333 798
Size: 333 465
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 798
Size: 343 32 423
Fragments arranged by size:
423 343 32
HincII GTy'rAC
Cuts at: 0 720 798
Size: 720 78
MscI TGG'CCA
Cuts at: 0 568 798
Size: 568 230
MslI CAynn'nnrTG
Cuts at: 0 67 286 532 798
Size: 67 219 246 266
Fragments arranged by size:
266 246 219 67
MspA1I CmG'CkG
Cuts at: 0 20 188 798
Size: 20 168 610
Fragments arranged by size:
610 168 20
NcoI C'CATG_G
Cuts at: 0 401 798
Size: 401 397
NspI r_CATG'y
Cuts at: 0 198 657 798
Size: 198 459 141
Fragments arranged by size:
459 198 141
PciI A'CATG_T
Cuts at: 0 194 798
Size: 194 604
PfoI T'CCnGG_A
Cuts at: 0 212 347 798
Size: 212 135 451
Fragments arranged by size:
451 212 135
PpuMI rG'GwC_Cy
Cuts at: 0 750 798
Size: 750 48
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 484 516 798
Size: 484 32 282
Fragments arranged by size:
484 282 32
SacI G_AGCT'C
Cuts at: 0 100 798
Size: 100 698
SacII CC_GC'GG
Cuts at: 0 189 798
Size: 189 609
SapI GCTCTTCn'nnn_
Cuts at: 0 562 798
Size: 562 236
SmlI C'TyrA_G
Cuts at: 0 730 798
Size: 730 68
SphI G_CATG'C
Cuts at: 0 657 798
Size: 657 141
StyI C'CwwG_G
Cuts at: 0 380 401 746 798
Size: 380 21 345 52
Fragments arranged by size:
380 345 52 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 64 162 798
Size: 64 98 636
Fragments arranged by size:
636 98 64
TatI w'GTAC_w
Cuts at: 0 140 734 798
Size: 140 594 64
Fragments arranged by size:
594 140 64
Tth111I GACn'n_nGTC
Cuts at: 0 12 798
Size: 12 786
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AloI AlwNI ApaLI
AvaI BanI BanII BbsI BciVI BglII Bme1580I BpmI
BpuEI BsaI BsaAI BsaHI BsaXI BseYI BsgI BsiHKAI
Bsp1286I BssSI BstYI BstZ17I BtgI BtgZI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I HincII
MscI MslI MspA1I NcoI NspI PciI PfoI PpuMI
PsrI SacI SacII SapI SmlI SphI StyI TaqII
TatI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BclI BfrBI BglI BlpI BmgBI
BmrI BmtI BplI Bpu10I BsaBI BsaWI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoNI
EcoRI EcoRV FalI FseI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TstI XbaI XcmI XhoI XmaI
XmnI ZraI