(Linear) (Six-base) MAPSORT of: KIBB8639.seq  Check: 281  from: 1  to: 984



KIBB8639.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     517     984 
   Size:        517     467
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     984 
   Size:        235     749
 
AlwNI CAG_nnn'CTG

Cuts at:      0     238     367     984 
   Size:        238     129     617

  Fragments arranged by size: 

                 617     238     129
 
ApoI r'AATT_y

Cuts at:      0     381     389     544     794     918     984 
   Size:        381       8     155     250     124      66

  Fragments arranged by size: 

                 381     250     155     124      66       8
 
BanII G_rGCy'C

Cuts at:      0     648     984 
   Size:        648     336
 
BmgBI CAC'GTC

Cuts at:      0     873     984 
   Size:        873     111
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     454     951     984 
   Size:        454     497      33

  Fragments arranged by size: 

                 497     454      33
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     635     984 
   Size:        635     349
 
Bsp1286I G_dGCh'C

Cuts at:      0     648     984 
   Size:        648     336
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     517     984 
   Size:        517     467
 
BsrDI GCAATG_nn'

Cuts at:      0     339     837     984 
   Size:        339     498     147

  Fragments arranged by size: 

                 498     339     147
 
BstYI r'GATC_y

Cuts at:      0      11     984 
   Size:         11     973
 
Bsu36I CC'TnA_GG

Cuts at:      0     201     984 
   Size:        201     783
 
BtgI C'CryG_G

Cuts at:      0     134     984 
   Size:        134     850
 
EarI CTCTTCn'nnn_

Cuts at:      0     464     875     984 
   Size:        464     411     109

  Fragments arranged by size: 

                 464     411     109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     984 
   Size:        235     749
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     113     984 
   Size:        113     871
 
EcoO109I rG'GnC_Cy

Cuts at:      0      75     593     984 
   Size:         75     518     391

  Fragments arranged by size: 

                 518     391      75
 
EcoRI G'AATT_C

Cuts at:      0     794     984 
   Size:        794     190
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     414     446     984 
   Size:        414      32     538

  Fragments arranged by size: 

                 538     414      32
 
MspA1I CmG'CkG

Cuts at:      0     229     984 
   Size:        229     755
 
PpuMI rG'GwC_Cy

Cuts at:      0     593     984 
   Size:        593     391
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     756     788     984 
   Size:        756      32     196

  Fragments arranged by size: 

                 756     196      32
 
PstI C_TGCA'G

Cuts at:      0     234     899     984 
   Size:        234     665      85

  Fragments arranged by size: 

                 665     234      85
 
PvuII CAG'CTG

Cuts at:      0     229     984 
   Size:        229     755
 
SapI GCTCTTCn'nnn_

Cuts at:      0     464     984 
   Size:        464     520
 
SexAI A'CCwGG_T

Cuts at:      0     589     984 
   Size:        589     395
 
SfcI C'TryA_G

Cuts at:      0     230     895     984 
   Size:        230     665      89

  Fragments arranged by size: 

                 665     230      89
 
SmlI C'TyrA_G

Cuts at:      0     433     930     984 
   Size:        433     497      54

  Fragments arranged by size: 

                 497     433      54
 
Tth111I GACn'n_nGTC

Cuts at:      0     591     984 
   Size:        591     393
 
XbaI T'CTAG_A

Cuts at:      0     846     984 
   Size:        846     138
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     ApoI    BanII    BmgBI    BpuEI     BsgI
 Bsp1286I    BspMI    BsrDI    BstYI   Bsu36I     BtgI     EarI  Eco57MI
    EcoNI EcoO109I    EcoRI    Hin4I   MspA1I    PpuMI     PsrI     PstI
    PvuII     SapI    SexAI     SfcI     SmlI  Tth111I     XbaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
     XcmI     XhoI     XmaI     XmnI     ZraI