(Linear) (Six-base) MAPSORT of: KIBB8636.seq  Check: 7006  from: 1  to: 1005



KIBB8636.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     376     807     892    1005 
   Size:        106     270     431      85     113

  Fragments arranged by size: 

                 431     270     113     106      85
 
AlwNI CAG_nnn'CTG

Cuts at:      0     399    1005 
   Size:        399     606
 
ApoI r'AATT_y

Cuts at:      0     100     290     298    1005 
   Size:        100     190       8     707

  Fragments arranged by size: 

                 707     190     100       8
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     734     767    1005 
   Size:        734      33     238

  Fragments arranged by size: 

                 734     238      33
 
BanII G_rGCy'C

Cuts at:      0     515    1005 
   Size:        515     490
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      86     223    1005 
   Size:         86     137     782

  Fragments arranged by size: 

                 782     137      86
 
BfrBI ATG'CAT

Cuts at:      0     788    1005 
   Size:        788     217
 
BlpI GC'TnA_GC

Cuts at:      0     514    1005 
   Size:        514     491
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      32     857    1005 
   Size:         32     825     148

  Fragments arranged by size: 

                 825     148      32
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     622    1005 
   Size:        622     383
 
BsiHKAI G_wGCw'C

Cuts at:      0     515    1005 
   Size:        515     490
 
BsmI GAATG_Cn'

Cuts at:      0     665    1005 
   Size:        665     340
 
Bsp1286I G_dGCh'C

Cuts at:      0     515    1005 
   Size:        515     490
 
BstEII G'GTnAC_C

Cuts at:      0     165    1005 
   Size:        165     840
 
BstYI r'GATC_y

Cuts at:      0     122    1005 
   Size:        122     883
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     824     859    1005 
   Size:        824      35     146

  Fragments arranged by size: 

                 824     146      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     177     878     939    1005 
   Size:        177     701      61      66

  Fragments arranged by size: 

                 701     177      66      61
 
EcoICRI GAG'CTC

Cuts at:      0     513    1005 
   Size:        513     492
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     376     807     892    1005 
   Size:        106     270     431      85     113

  Fragments arranged by size: 

                 431     270     113     106      85
 
EcoRI G'AATT_C

Cuts at:      0     298    1005 
   Size:        298     707
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     871    1005 
   Size:        871     134
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     149     181    1005 
   Size:        149      32     824

  Fragments arranged by size: 

                 824     149      32
 
HincII GTy'rAC

Cuts at:      0     483     819     963    1005 
   Size:        483     336     144      42

  Fragments arranged by size: 

                 483     336     144      42
 
HindIII A'AGCT_T

Cuts at:      0     141    1005 
   Size:        141     864
 
HpaI GTT'AAC

Cuts at:      0     483    1005 
   Size:        483     522
 
NsiI A_TGCA'T

Cuts at:      0     790    1005 
   Size:        790     215
 
NspI r_CATG'y

Cuts at:      0     594     707    1005 
   Size:        594     113     298

  Fragments arranged by size: 

                 594     298     113
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     373    1005 
   Size:        373     632
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     389     421    1005 
   Size:        389      32     584

  Fragments arranged by size: 

                 584     389      32
 
SacI G_AGCT'C

Cuts at:      0     515    1005 
   Size:        515     490
 
SapI GCTCTTCn'nnn_

Cuts at:      0     939    1005 
   Size:        939      66
 
SfcI C'TryA_G

Cuts at:      0     253     650    1005 
   Size:        253     397     355

  Fragments arranged by size: 

                 397     355     253
 
SmlI C'TyrA_G

Cuts at:      0     601    1005 
   Size:        601     404
 
StyI C'CwwG_G

Cuts at:      0     434    1005 
   Size:        434     571
 
TatI w'GTAC_w

Cuts at:      0      19    1005 
   Size:         19     986
 
XmnI GAAnn'nnTTC

Cuts at:      0     401     638    1005 
   Size:        401     237     367

  Fragments arranged by size: 

                 401     367     237
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BaeI    BanII     BbsI    BfrBI     BlpI
     BmrI    BpuEI  BsiHKAI     BsmI Bsp1286I   BstEII    BstYI    CspCI
     EarI  EcoICRI  Eco57MI    EcoRI     FalI    Hin4I   HincII  HindIII
     HpaI     NsiI     NspI    PflMI     PpiI     SacI     SapI     SfcI
     SmlI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII Bme1580I    BmgBI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI
 EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI