Restriction map for
pF1KB8625
(Linear) (Six-base) MAPSORT of: KIBB8625.seq Check: 2038 from: 1 to: 993
KIBB8625.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 496 893 958 993
Size: 81 415 397 65 35
Fragments arranged by size:
415 397 81 65 35
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 10 42 993
Size: 10 32 951
Fragments arranged by size:
951 32 10
AlwNI CAG_nnn'CTG
Cuts at: 0 531 993
Size: 531 462
ApaI G_GGCC'C
Cuts at: 0 305 993
Size: 305 688
ApoI r'AATT_y
Cuts at: 0 816 985 993
Size: 816 169 8
Fragments arranged by size:
816 169 8
AvrII C'CTAG_G
Cuts at: 0 486 993
Size: 486 507
BanI G'GyrC_C
Cuts at: 0 614 993
Size: 614 379
BanII G_rGCy'C
Cuts at: 0 225 305 632 665 694 993
Size: 225 80 327 33 29 299
Fragments arranged by size:
327 299 225 80 33 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 208 993
Size: 208 785
BciVI GTATCCnnnnn_n'
Cuts at: 0 423 993
Size: 423 570
BglI GCCn_nnn'nGGC
Cuts at: 0 271 993
Size: 271 722
BlpI GC'TnA_GC
Cuts at: 0 53 993
Size: 53 940
Bme1580I G_kGCm'C
Cuts at: 0 305 993
Size: 305 688
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 708 993
Size: 708 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 993
Size: 754 239
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 616 993
Size: 379 237 377
Fragments arranged by size:
379 377 237
BsaI GGTCTCn'nnnn_
Cuts at: 0 286 993
Size: 286 707
BsaBI GATnn'nnATC
Cuts at: 0 956 993
Size: 956 37
BsaWI w'CCGG_w
Cuts at: 0 960 993
Size: 960 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 10 40 679 709 993
Size: 10 30 639 30 284
Fragments arranged by size:
639 284 30 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 34 149 460 700 993
Size: 31 3 115 311 240 293
Fragments arranged by size:
311 293 240 115 31 3
BseYI C'CCAG_C
Cuts at: 0 389 993
Size: 389 604
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 993
Size: 94 899
BsiHKAI G_wGCw'C
Cuts at: 0 665 694 993
Size: 665 29 299
Fragments arranged by size:
665 299 29
Bsp1286I G_dGCh'C
Cuts at: 0 225 305 632 665 694 993
Size: 225 80 327 33 29 299
Fragments arranged by size:
327 299 225 80 33 29
BspEI T'CCGG_A
Cuts at: 0 960 993
Size: 960 33
BspHI T'CATG_A
Cuts at: 0 381 993
Size: 381 612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 512 993
Size: 512 481
BsrBI CCG'CTC
Cuts at: 0 24 240 258 993
Size: 24 216 18 735
Fragments arranged by size:
735 216 24 18
BssSI C'ACGA_G
Cuts at: 0 61 693 993
Size: 61 632 300
Fragments arranged by size:
632 300 61
Bsu36I CC'TnA_GG
Cuts at: 0 297 993
Size: 297 696
BtgI C'CryG_G
Cuts at: 0 47 893 993
Size: 47 846 100
Fragments arranged by size:
846 100 47
DrdI GACnn_nn'nnGTC
Cuts at: 0 550 993
Size: 550 443
EaeI y'GGCC_r
Cuts at: 0 44 263 272 993
Size: 44 219 9 721
Fragments arranged by size:
721 219 44 9
EarI CTCTTCn'nnn_
Cuts at: 0 211 323 993
Size: 211 112 670
Fragments arranged by size:
670 211 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 20 268 993
Size: 20 248 725
Fragments arranged by size:
725 248 20
EcoICRI GAG'CTC
Cuts at: 0 663 692 993
Size: 663 29 301
Fragments arranged by size:
663 301 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 496 754 893 958 993
Size: 81 415 258 139 65 35
Fragments arranged by size:
415 258 139 81 65 35
EcoO109I rG'GnC_Cy
Cuts at: 0 301 302 993
Size: 301 1 691
Fragments arranged by size:
691 301 1
EcoRI G'AATT_C
Cuts at: 0 816 993
Size: 816 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 714 746 993
Size: 714 32 247
Fragments arranged by size:
714 247 32
HindIII A'AGCT_T
Cuts at: 0 157 993
Size: 157 836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 993
Size: 515 478
MscI TGG'CCA
Cuts at: 0 46 274 993
Size: 46 228 719
Fragments arranged by size:
719 228 46
MslI CAynn'nnrTG
Cuts at: 0 595 993
Size: 595 398
MspA1I CmG'CkG
Cuts at: 0 192 518 609 993
Size: 192 326 91 384
Fragments arranged by size:
384 326 192 91
NcoI C'CATG_G
Cuts at: 0 47 893 993
Size: 47 846 100
Fragments arranged by size:
846 100 47
NspI r_CATG'y
Cuts at: 0 39 993
Size: 39 954
PasI CC'CwG_GG
Cuts at: 0 418 993
Size: 418 575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 993
Size: 10 32 951
Fragments arranged by size:
951 32 10
PshAI GACnn'nnGTC
Cuts at: 0 888 993
Size: 888 105
PspOMI G'GGCC_C
Cuts at: 0 301 993
Size: 301 692
PstI C_TGCA'G
Cuts at: 0 347 993
Size: 347 646
PvuII CAG'CTG
Cuts at: 0 192 993
Size: 192 801
SacI G_AGCT'C
Cuts at: 0 665 694 993
Size: 665 29 299
Fragments arranged by size:
665 299 29
SfcI C'TryA_G
Cuts at: 0 343 993
Size: 343 650
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 271 993
Size: 271 722
SmlI C'TyrA_G
Cuts at: 0 394 631 993
Size: 394 237 362
Fragments arranged by size:
394 362 237
StyI C'CwwG_G
Cuts at: 0 47 275 486 893 977 993
Size: 47 228 211 407 84 16
Fragments arranged by size:
407 228 211 84 47 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 679 711 993
Size: 679 32 282
Fragments arranged by size:
679 282 32
Tth111I GACn'n_nGTC
Cuts at: 0 531 993
Size: 531 462
XmnI GAAnn'nnTTC
Cuts at: 0 91 943 993
Size: 91 852 50
Fragments arranged by size:
852 91 50
Enzymes that do cut:
AcuI AloI AlwNI ApaI ApoI AvrII BanI BanII
BbsI BciVI BglI BlpI Bme1580I BplI BpmI BpuEI
BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BssSI Bsu36I BtgI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
Hin4I HindIII MmeI MscI MslI MspA1I NcoI NspI
PasI PpiI PshAI PspOMI PstI PvuII SacI SfcI
SfiI SmlI StyI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BbeI BbvCI BcgI BclI BfrBI
BglII BmgBI BmrI BmtI Bpu10I BsaAI BsaHI BsiEI
BsiWI BsmI BsmBI BsrDI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII EagI EcoNI EcoRV FalI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpuMI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI XbaI XcmI XhoI XmaI ZraI