Restriction map for
pF1KB8622
(Linear) (Six-base) MAPSORT of: KIBB8622.seq Check: 6184 from: 1 to: 2517
KIBB8622.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1013 2517
Size: 1013 1504
AatII G_ACGT'C
Cuts at: 0 422 1385 2517
Size: 422 963 1132
Fragments arranged by size:
1132 963 422
AccI GT'mk_AC
Cuts at: 0 422 2517
Size: 422 2095
AclI AA'CG_TT
Cuts at: 0 2283 2517
Size: 2283 234
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 888 952 2245 2517
Size: 587 301 64 1293 272
Fragments arranged by size:
1293 587 301 272 64
AfeI AGC'GCT
Cuts at: 0 2086 2517
Size: 2086 431
AflIII A'CryG_T
Cuts at: 0 761 1556 1625 2517
Size: 761 795 69 892
Fragments arranged by size:
892 795 761 69
AhdI GACnn_n'nnGTC
Cuts at: 0 1842 2517
Size: 1842 675
AleI CACnn'nnGTG
Cuts at: 0 2344 2517
Size: 2344 173
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 64 2517
Size: 64 2453
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1208 1240 2517
Size: 1208 32 1277
Fragments arranged by size:
1277 1208 32
AlwNI CAG_nnn'CTG
Cuts at: 0 108 138 207 885 2517
Size: 108 30 69 678 1632
Fragments arranged by size:
1632 678 108 69 30
ApaLI G'TGCA_C
Cuts at: 0 924 2517
Size: 924 1593
ApoI r'AATT_y
Cuts at: 0 253 949 1147 1167 1189 2176 2517
Size: 253 696 198 20 22 987 341
Fragments arranged by size:
987 696 341 253 198 22 20
AvaI C'yCGr_G
Cuts at: 0 1780 1958 2276 2369 2517
Size: 1780 178 318 93 148
Fragments arranged by size:
1780 318 178 148 93
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1614 1647 2245 2278 2517
Size: 1614 33 598 33 239
Fragments arranged by size:
1614 598 239 33 33
BamHI G'GATC_C
Cuts at: 0 2153 2517
Size: 2153 364
BanI G'GyrC_C
Cuts at: 0 1606 2517
Size: 1606 911
BanII G_rGCy'C
Cuts at: 0 664 685 1322 2517
Size: 664 21 637 1195
Fragments arranged by size:
1195 664 637 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 81 696 792 1203 2517
Size: 81 615 96 411 1314
Fragments arranged by size:
1314 615 411 96 81
BbvCI CC'TCA_GC
Cuts at: 0 1828 2517
Size: 1828 689
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1957 1991 2517
Size: 1957 34 526
Fragments arranged by size:
1957 526 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1615 2517
Size: 1615 902
BclI T'GATC_A
Cuts at: 0 725 2057 2517
Size: 725 1332 460
Fragments arranged by size:
1332 725 460
BglI GCCn_nnn'nGGC
Cuts at: 0 1050 2296 2429 2517
Size: 1050 1246 133 88
Fragments arranged by size:
1246 1050 133 88
BglII A'GATC_T
Cuts at: 0 1704 2517
Size: 1704 813
BlpI GC'TnA_GC
Cuts at: 0 679 2081 2517
Size: 679 1402 436
Fragments arranged by size:
1402 679 436
Bme1580I G_kGCm'C
Cuts at: 0 928 1609 1653 2517
Size: 928 681 44 864
Fragments arranged by size:
928 864 681 44
BmgBI CAC'GTC
Cuts at: 0 762 1815 2406 2517
Size: 762 1053 591 111
Fragments arranged by size:
1053 762 591 111
BmrI ACTGGGnnnn_n'
Cuts at: 0 195 2308 2517
Size: 195 2113 209
Fragments arranged by size:
2113 209 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 616 655 736 1204 2212 2277 2286
Size: 454 162 39 81 468 1008 65 9
Cuts at: 2286 2517
Size: 231
Fragments arranged by size:
1008 468 454 231 162 81 65 39
9
Bpu10I CC'TnA_GC
Cuts at: 0 1828 2517
Size: 1828 689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1152 1306 1840 2014 2242 2517
Size: 154 998 154 534 174 228 275
Fragments arranged by size:
998 534 275 228 174 154 154
BsaI GGTCTCn'nnnn_
Cuts at: 0 391 601 1120 2429 2452 2517
Size: 391 210 519 1309 23 65
Fragments arranged by size:
1309 519 391 210 65 23
BsaAI yAC'GTr
Cuts at: 0 2496 2517
Size: 2496 21
BsaBI GATnn'nnATC
Cuts at: 0 1099 2158 2517
Size: 1099 1059 359
Fragments arranged by size:
1099 1059 359
BsaHI Gr'CG_yC
Cuts at: 0 419 1382 1643 1712 2517
Size: 419 963 261 69 805
Fragments arranged by size:
963 805 419 261 69
BsaWI w'CCGG_w
Cuts at: 0 1226 1717 2517
Size: 1226 491 800
Fragments arranged by size:
1226 800 491
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 133 163 2517
Size: 133 30 2354
Fragments arranged by size:
2354 133 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 409 451 934 2517
Size: 409 42 483 1583
Fragments arranged by size:
1583 483 409 42
BseYI C'CCAG_C
Cuts at: 0 1770 1897 2517
Size: 1770 127 620
Fragments arranged by size:
1770 620 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 334 559 2517
Size: 124 210 225 1958
Fragments arranged by size:
1958 225 210 124
BsiEI CG_ry'CG
Cuts at: 0 24 83 190 1951 2517
Size: 24 59 107 1761 566
Fragments arranged by size:
1761 566 107 59 24
BsiHKAI G_wGCw'C
Cuts at: 0 860 928 995 1322 2517
Size: 860 68 67 327 1195
Fragments arranged by size:
1195 860 327 68 67
BsiWI C'GTAC_G
Cuts at: 0 2492 2517
Size: 2492 25
Bsp1286I G_dGCh'C
Cuts at: 0 664 685 860 928 995 1322 1609 1653
Size: 664 21 175 68 67 327 287 44
Cuts at: 1653 2517
Size: 864
Fragments arranged by size:
864 664 327 287 175 68 67 44
21
BspEI T'CCGG_A
Cuts at: 0 1717 2517
Size: 1717 800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 974 1013 1984 2033 2517
Size: 974 39 971 49 484
Fragments arranged by size:
974 971 484 49 39
BsrBI CCG'CTC
Cuts at: 0 1723 2067 2517
Size: 1723 344 450
Fragments arranged by size:
1723 450 344
BsrFI r'CCGG_y
Cuts at: 0 1946 2306 2517
Size: 1946 360 211
Fragments arranged by size:
1946 360 211
BsrGI T'GTAC_A
Cuts at: 0 1097 1553 2517
Size: 1097 456 964
Fragments arranged by size:
1097 964 456
BstAPI GCAn_nnn'nTGC
Cuts at: 0 108 966 990 1586 2517
Size: 108 858 24 596 931
Fragments arranged by size:
931 858 596 108 24
BstXI CCAn_nnnn'nTGG
Cuts at: 0 555 1743 2517
Size: 555 1188 774
Fragments arranged by size:
1188 774 555
BstYI r'GATC_y
Cuts at: 0 599 1704 2153 2414 2517
Size: 599 1105 449 261 103
Fragments arranged by size:
1105 599 449 261 103
Bsu36I CC'TnA_GG
Cuts at: 0 748 1600 2517
Size: 748 852 917
Fragments arranged by size:
917 852 748
BtgI C'CryG_G
Cuts at: 0 83 329 1761 1849 2517
Size: 83 246 1432 88 668
Fragments arranged by size:
1432 668 246 88 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 342 1108 2517
Size: 342 766 1409
Fragments arranged by size:
1409 766 342
BtsI GCAGTG_nn'
Cuts at: 0 13 1973 2517
Size: 13 1960 544
Fragments arranged by size:
1960 544 13
ClaI AT'CG_AT
Cuts at: 0 35 2517
Size: 35 2482
DraIII CAC_nnn'GTG
Cuts at: 0 66 2517
Size: 66 2451
DrdI GACnn_nn'nnGTC
Cuts at: 0 418 1282 2517
Size: 418 864 1235
Fragments arranged by size:
1235 864 418
EaeI y'GGCC_r
Cuts at: 0 21 80 187 443 791 887 1496 1745
Size: 21 59 107 256 348 96 609 249
Cuts at: 1745 1948 2348 2517
Size: 203 400 169
Fragments arranged by size:
609 400 348 256 249 203 169 107
96 59 21
EagI C'GGCC_G
Cuts at: 0 21 80 187 1948 2517
Size: 21 59 107 1761 569
Fragments arranged by size:
1761 569 107 59 21
EarI CTCTTCn'nnn_
Cuts at: 0 301 1462 2495 2517
Size: 301 1161 1033 22
Fragments arranged by size:
1161 1033 301 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 703 2447 2517
Size: 703 1744 70
Fragments arranged by size:
1744 703 70
EcoICRI GAG'CTC
Cuts at: 0 1320 2517
Size: 1320 1197
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 587 616 655 736 888 952 1204
Size: 454 133 29 39 81 152 64 252
Cuts at: 1204 2212 2245 2277 2286 2517
Size: 1008 33 32 9 231
Fragments arranged by size:
1008 454 252 231 152 133 81 64
39 33 32 29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 100 463 727 2517
Size: 100 363 264 1790
Fragments arranged by size:
1790 363 264 100
EcoO109I rG'GnC_Cy
Cuts at: 0 45 120 720 1732 2048 2297 2517
Size: 45 75 600 1012 316 249 220
Fragments arranged by size:
1012 600 316 249 220 75 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1146 1452 2250 2517
Size: 1146 306 798 267
Fragments arranged by size:
1146 798 306 267
HaeII r_GCGC'y
Cuts at: 0 109 2088 2517
Size: 109 1979 429
Fragments arranged by size:
1979 429 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1828 1860 2517
Size: 1828 32 657
Fragments arranged by size:
1828 657 32
HincII GTy'rAC
Cuts at: 0 1239 2517
Size: 1239 1278
MscI TGG'CCA
Cuts at: 0 793 889 1498 1747 2350 2517
Size: 793 96 609 249 603 167
Fragments arranged by size:
793 609 603 249 167 96
MslI CAynn'nnrTG
Cuts at: 0 529 553 760 2131 2344 2517
Size: 529 24 207 1371 213 173
Fragments arranged by size:
1371 529 213 207 173 24
MspA1I CmG'CkG
Cuts at: 0 26 135 331 700 2045 2244 2344 2517
Size: 26 109 196 369 1345 199 100 173
Fragments arranged by size:
1345 369 199 196 173 109 100 26
NaeI GCC'GGC
Cuts at: 0 1948 2517
Size: 1948 569
NcoI C'CATG_G
Cuts at: 0 1761 2517
Size: 1761 756
NgoMIV G'CCGG_C
Cuts at: 0 1946 2517
Size: 1946 571
NotI GC'GGCC_GC
Cuts at: 0 21 2517
Size: 21 2496
NspI r_CATG'y
Cuts at: 0 1629 2517
Size: 1629 888
PciI A'CATG_T
Cuts at: 0 1625 2517
Size: 1625 892
PflMI CCAn_nnn'nTGG
Cuts at: 0 66 885 2517
Size: 66 819 1632
Fragments arranged by size:
1632 819 66
PfoI T'CCnGG_A
Cuts at: 0 602 938 2189 2517
Size: 602 336 1251 328
Fragments arranged by size:
1251 602 336 328
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1327 1359 2517
Size: 1327 32 1158
Fragments arranged by size:
1327 1158 32
PpuMI rG'GwC_Cy
Cuts at: 0 45 120 720 1732 2048 2517
Size: 45 75 600 1012 316 469
Fragments arranged by size:
1012 600 469 316 75 45
PstI C_TGCA'G
Cuts at: 0 847 1704 2517
Size: 847 857 813
Fragments arranged by size:
857 847 813
PvuII CAG'CTG
Cuts at: 0 135 700 2244 2344 2517
Size: 135 565 1544 100 173
Fragments arranged by size:
1544 565 173 135 100
SacI G_AGCT'C
Cuts at: 0 1322 2517
Size: 1322 1195
SacII CC_GC'GG
Cuts at: 0 332 2517
Size: 332 2185
ScaI AGT'ACT
Cuts at: 0 622 841 2206 2517
Size: 622 219 1365 311
Fragments arranged by size:
1365 622 311 219
SexAI A'CCwGG_T
Cuts at: 0 1728 2517
Size: 1728 789
SfcI C'TryA_G
Cuts at: 0 843 1160 1308 1700 2418 2517
Size: 843 317 148 392 718 99
Fragments arranged by size:
843 718 392 317 148 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2296 2517
Size: 2296 221
SmaI CCC'GGG
Cuts at: 0 1782 1960 2278 2517
Size: 1782 178 318 239
Fragments arranged by size:
1782 318 239 178
SmlI C'TyrA_G
Cuts at: 0 169 1131 1321 1819 2029 2257 2517
Size: 169 962 190 498 210 228 260
Fragments arranged by size:
962 498 260 228 210 190 169
SnaBI TAC'GTA
Cuts at: 0 2496 2517
Size: 2496 21
StuI AGG'CCT
Cuts at: 0 381 2517
Size: 381 2136
StyI C'CwwG_G
Cuts at: 0 794 1761 2517
Size: 794 967 756
Fragments arranged by size:
967 794 756
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 625 1372 2517
Size: 625 747 1145
Fragments arranged by size:
1145 747 625
TatI w'GTAC_w
Cuts at: 0 620 839 1097 1553 2204 2517
Size: 620 219 258 456 651 313
Fragments arranged by size:
651 620 456 313 258 219
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 133 165 920 952 2517
Size: 133 32 755 32 1565
Fragments arranged by size:
1565 755 133 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 118 481 1730 2517
Size: 118 363 1249 787
Fragments arranged by size:
1249 787 363 118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 63 253 1086 2517
Size: 63 190 833 1431
Fragments arranged by size:
1431 833 190 63
XmaI C'CCGG_G
Cuts at: 0 1780 1958 2276 2517
Size: 1780 178 318 241
Fragments arranged by size:
1780 318 241 178
XmnI GAAnn'nnTTC
Cuts at: 0 821 2517
Size: 821 1696
ZraI GAC'GTC
Cuts at: 0 420 1383 2517
Size: 420 963 1134
Fragments arranged by size:
1134 963 420
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AvaI BaeI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII MscI MslI MspA1I NaeI NcoI NgoMIV NotI
NspI PciI PflMI PfoI PpiI PpuMI PstI PvuII
SacI SacII ScaI SexAI SfcI SfiI SmaI SmlI
SnaBI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AflII AgeI ApaI AscI AseI AsiSI AvrII
BbeI BfrBI BmtI BplI BsmI BsmBI BspHI BsrDI
BssHII BssSI BstBI BstEII BstZ17I CspCI DraI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MmeI NarI NdeI NheI NruI NsiI
PacI PasI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SalI SanDI SapI SbfI SfoI
SgfI SgrAI SpeI SphI SrfI SspI SwaI XbaI
XhoI