Restriction map for
pF1KB8619
(Linear) (Six-base) MAPSORT of: KIBB8619.seq Check: 7823 from: 1 to: 948
KIBB8619.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 948
Size: 222 726
AlwNI CAG_nnn'CTG
Cuts at: 0 282 852 936 948
Size: 282 570 84 12
Fragments arranged by size:
570 282 84 12
ApaI G_GGCC'C
Cuts at: 0 587 948
Size: 587 361
ApoI r'AATT_y
Cuts at: 0 31 948
Size: 31 917
BanI G'GyrC_C
Cuts at: 0 335 525 948
Size: 335 190 423
Fragments arranged by size:
423 335 190
BanII G_rGCy'C
Cuts at: 0 136 587 948
Size: 136 451 361
Fragments arranged by size:
451 361 136
BbsI GAAGACnn'nnnn_
Cuts at: 0 627 948
Size: 627 321
BglI GCCn_nnn'nGGC
Cuts at: 0 391 817 948
Size: 391 426 131
Fragments arranged by size:
426 391 131
Bme1580I G_kGCm'C
Cuts at: 0 116 530 587 948
Size: 116 414 57 361
Fragments arranged by size:
414 361 116 57
BmgBI CAC'GTC
Cuts at: 0 264 948
Size: 264 684
BmrI ACTGGGnnnn_n'
Cuts at: 0 465 948
Size: 465 483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 948
Size: 619 329
Bpu10I CC'TnA_GC
Cuts at: 0 135 948
Size: 135 813
BsaAI yAC'GTr
Cuts at: 0 117 948
Size: 117 831
BsaWI w'CCGG_w
Cuts at: 0 287 858 948
Size: 287 571 90
Fragments arranged by size:
571 287 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 592 607 622 637 948
Size: 592 15 15 15 311
Fragments arranged by size:
592 311 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 184 187 456 863 866 948
Size: 52 132 3 269 407 3 82
Fragments arranged by size:
407 269 132 82 52 3 3
BsiEI CG_ry'CG
Cuts at: 0 11 355 948
Size: 11 344 593
Fragments arranged by size:
593 344 11
Bsp1286I G_dGCh'C
Cuts at: 0 116 136 530 587 948
Size: 116 20 394 57 361
Fragments arranged by size:
394 361 116 57 20
BspEI T'CCGG_A
Cuts at: 0 858 948
Size: 858 90
BstAPI GCAn_nnn'nTGC
Cuts at: 0 342 509 948
Size: 342 167 439
Fragments arranged by size:
439 342 167
BstEII G'GTnAC_C
Cuts at: 0 483 948
Size: 483 465
BstXI CCAn_nnnn'nTGG
Cuts at: 0 771 948
Size: 771 177
BstYI r'GATC_y
Cuts at: 0 357 377 948
Size: 357 20 571
Fragments arranged by size:
571 357 20
BtgI C'CryG_G
Cuts at: 0 128 488 644 746 791 821 948
Size: 128 360 156 102 45 30 127
Fragments arranged by size:
360 156 128 127 102 45 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 891 948
Size: 891 57
BtsI GCAGTG_nn'
Cuts at: 0 301 688 948
Size: 301 387 260
Fragments arranged by size:
387 301 260
DraIII CAC_nnn'GTG
Cuts at: 0 483 948
Size: 483 465
EaeI y'GGCC_r
Cuts at: 0 8 125 318 352 404 773 818 948
Size: 8 117 193 34 52 369 45 130
Fragments arranged by size:
369 193 130 117 52 45 34 8
EagI C'GGCC_G
Cuts at: 0 8 352 948
Size: 8 344 596
Fragments arranged by size:
596 344 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 619 948
Size: 222 397 329
Fragments arranged by size:
397 329 222
EcoO109I rG'GnC_Cy
Cuts at: 0 279 447 583 584 948
Size: 279 168 136 1 364
Fragments arranged by size:
364 279 168 136 1
EcoRI G'AATT_C
Cuts at: 0 31 948
Size: 31 917
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 855 948
Size: 855 93
HaeII r_GCGC'y
Cuts at: 0 700 948
Size: 700 248
MscI TGG'CCA
Cuts at: 0 320 775 820 948
Size: 320 455 45 128
Fragments arranged by size:
455 320 128 45
MslI CAynn'nnrTG
Cuts at: 0 643 667 948
Size: 643 24 281
Fragments arranged by size:
643 281 24
MspA1I CmG'CkG
Cuts at: 0 99 948
Size: 99 849
NcoI C'CATG_G
Cuts at: 0 644 821 948
Size: 644 177 127
Fragments arranged by size:
644 177 127
PasI CC'CwG_GG
Cuts at: 0 705 948
Size: 705 243
PfoI T'CCnGG_A
Cuts at: 0 281 569 596 948
Size: 281 288 27 352
Fragments arranged by size:
352 288 281 27
PmlI CAC'GTG
Cuts at: 0 117 948
Size: 117 831
PpuMI rG'GwC_Cy
Cuts at: 0 279 447 948
Size: 279 168 501
Fragments arranged by size:
501 279 168
PspOMI G'GGCC_C
Cuts at: 0 583 948
Size: 583 365
PstI C_TGCA'G
Cuts at: 0 200 683 948
Size: 200 483 265
Fragments arranged by size:
483 265 200
PvuII CAG'CTG
Cuts at: 0 99 948
Size: 99 849
SanDI GG'GwC_CC
Cuts at: 0 447 948
Size: 447 501
SbfI CC_TGCA'GG
Cuts at: 0 200 948
Size: 200 748
SfcI C'TryA_G
Cuts at: 0 196 462 679 948
Size: 196 266 217 269
Fragments arranged by size:
269 266 217 196
StyI C'CwwG_G
Cuts at: 0 644 673 821 948
Size: 644 29 148 127
Fragments arranged by size:
644 148 127 29
TatI w'GTAC_w
Cuts at: 0 836 948
Size: 836 112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 592 624 948
Size: 592 32 324
Fragments arranged by size:
592 324 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 51 948
Size: 51 897
Enzymes that do cut:
AcuI AlwNI ApaI ApoI BanI BanII BbsI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI BsaWI BsaXI
BseRI BsiEI Bsp1286I BspEI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI DraIII EaeI EagI Eco57MI EcoO109I
EcoRI FalI HaeII MscI MslI MspA1I NcoI PasI
PfoI PmlI PpuMI PspOMI PstI PvuII SanDI SbfI
SfcI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmtI BplI BpuEI
BsaI BsaBI BsaHI BseYI BsgI BsiHKAI BsiWI BsmI
BsmBI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EarI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XhoI XmaI XmnI ZraI