Restriction map for
pF1KB8616
(Linear) (Six-base) MAPSORT of: KIBB8616.seq Check: 6325 from: 1 to: 1104
KIBB8616.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AatII G_ACGT'C
Cuts at: 0 402 1104
Size: 402 702
Acc65I G'GTAC_C
Cuts at: 0 483 1104
Size: 483 621
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 518 919 1031 1104
Size: 413 105 401 112 73
Fragments arranged by size:
413 401 112 105 73
AhdI GACnn_n'nnGTC
Cuts at: 0 1086 1104
Size: 1086 18
ApaI G_GGCC'C
Cuts at: 0 460 1104
Size: 460 644
ApoI r'AATT_y
Cuts at: 0 115 300 742 1099 1104
Size: 115 185 442 357 5
Fragments arranged by size:
442 357 185 115 5
AvaI C'yCGr_G
Cuts at: 0 37 1104
Size: 37 1067
BanI G'GyrC_C
Cuts at: 0 483 1104
Size: 483 621
BanII G_rGCy'C
Cuts at: 0 29 460 1104
Size: 29 431 644
Fragments arranged by size:
644 431 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 1037 1104
Size: 1037 67
BglII A'GATC_T
Cuts at: 0 149 1104
Size: 149 955
Bme1580I G_kGCm'C
Cuts at: 0 460 731 1104
Size: 460 271 373
Fragments arranged by size:
460 373 271
BmrI ACTGGGnnnn_n'
Cuts at: 0 718 1104
Size: 718 386
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 824 1104
Size: 824 280
Bpu10I CC'TnA_GC
Cuts at: 0 250 1104
Size: 250 854
BsaI GGTCTCn'nnnn_
Cuts at: 0 812 1104
Size: 812 292
BsaHI Gr'CG_yC
Cuts at: 0 399 1104
Size: 399 705
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 976 1104
Size: 976 128
BseYI C'CCAG_C
Cuts at: 0 459 731 1104
Size: 459 272 373
Fragments arranged by size:
459 373 272
BsiHKAI G_wGCw'C
Cuts at: 0 7 1104
Size: 7 1097
Bsp1286I G_dGCh'C
Cuts at: 0 7 29 460 731 1104
Size: 7 22 431 271 373
Fragments arranged by size:
431 373 271 22 7
BsrBI CCG'CTC
Cuts at: 0 828 1104
Size: 828 276
BsrDI GCAATG_nn'
Cuts at: 0 182 599 789 1104
Size: 182 417 190 315
Fragments arranged by size:
417 315 190 182
BstXI CCAn_nnnn'nTGG
Cuts at: 0 394 802 1104
Size: 394 408 302
Fragments arranged by size:
408 394 302
BstYI r'GATC_y
Cuts at: 0 149 1104
Size: 149 955
BtgI C'CryG_G
Cuts at: 0 15 517 1104
Size: 15 502 587
Fragments arranged by size:
587 502 15
DraI TTT'AAA
Cuts at: 0 747 1104
Size: 747 357
DrdI GACnn_nn'nnGTC
Cuts at: 0 398 1104
Size: 398 706
EarI CTCTTCn'nnn_
Cuts at: 0 416 793 818 1104
Size: 416 377 25 286
Fragments arranged by size:
416 377 286 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 518 919 1031 1104
Size: 413 105 401 112 73
Fragments arranged by size:
413 401 112 105 73
EcoO109I rG'GnC_Cy
Cuts at: 0 456 1104
Size: 456 648
EcoRI G'AATT_C
Cuts at: 0 300 1099 1104
Size: 300 799 5
Fragments arranged by size:
799 300 5
FspI TGC'GCA
Cuts at: 0 106 1104
Size: 106 998
HaeII r_GCGC'y
Cuts at: 0 90 1104
Size: 90 1014
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 1014 1046 1072 1075 1104
Size: 310 32 672 32 26 3 29
Fragments arranged by size:
672 310 32 32 29 26 3
KpnI G_GTAC'C
Cuts at: 0 487 1104
Size: 487 617
MfeI C'AATT_G
Cuts at: 0 263 1104
Size: 263 841
MslI CAynn'nnrTG
Cuts at: 0 697 854 1104
Size: 697 157 250
Fragments arranged by size:
697 250 157
MspA1I CmG'CkG
Cuts at: 0 17 54 519 735 906 1104
Size: 17 37 465 216 171 198
Fragments arranged by size:
465 216 198 171 37 17
NdeI CA'TA_TG
Cuts at: 0 893 1104
Size: 893 211
PfoI T'CCnGG_A
Cuts at: 0 336 1104
Size: 336 768
PsiI TTA'TAA
Cuts at: 0 1028 1104
Size: 1028 76
PspOMI G'GGCC_C
Cuts at: 0 456 1104
Size: 456 648
PvuII CAG'CTG
Cuts at: 0 735 906 1104
Size: 735 171 198
Fragments arranged by size:
735 198 171
SacII CC_GC'GG
Cuts at: 0 18 520 1104
Size: 18 502 584
Fragments arranged by size:
584 502 18
SapI GCTCTTCn'nnn_
Cuts at: 0 818 1104
Size: 818 286
ScaI AGT'ACT
Cuts at: 0 642 1104
Size: 642 462
SfcI C'TryA_G
Cuts at: 0 350 1104
Size: 350 754
SpeI A'CTAG_T
Cuts at: 0 330 1104
Size: 330 774
SspI AAT'ATT
Cuts at: 0 1011 1104
Size: 1011 93
StuI AGG'CCT
Cuts at: 0 659 1104
Size: 659 445
StyI C'CwwG_G
Cuts at: 0 609 926 1104
Size: 609 317 178
Fragments arranged by size:
609 317 178
TatI w'GTAC_w
Cuts at: 0 440 640 1104
Size: 440 200 464
Fragments arranged by size:
464 440 200
ZraI GAC'GTC
Cuts at: 0 400 1104
Size: 400 704
Enzymes that do cut:
AatII Acc65I AcuI AhdI ApaI ApoI AvaI BanI
BanII BbsI BglII Bme1580I BmrI BplI Bpu10I BsaI
BsaHI BseRI BseYI BsiHKAI Bsp1286I BsrBI BsrDI BstXI
BstYI BtgI DraI DrdI EarI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I KpnI MfeI MslI MspA1I NdeI
PfoI PsiI PspOMI PvuII SacII SapI ScaI SfcI
SpeI SspI StuI StyI TatI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmtI BpmI BpuEI BsaAI BsaBI
BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII
EaeI EagI EciI EcoICRI EcoNI EcoRV FalI FseI
FspAI HincII HindIII HpaI KasI MluI MmeI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PspXI PsrI PstI PvuI RsrII SacI
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SphI SrfI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI XmnI