(Linear) (Six-base) MAPSORT of: KIBB8599.seq  Check: 7035  from: 1  to: 732



KIBB8599.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     384     732 
   Size:        184     200     348

  Fragments arranged by size: 

                 348     200     184
 
AvaI C'yCGr_G

Cuts at:      0      15     732 
   Size:         15     717
 
BanI G'GyrC_C

Cuts at:      0     220     732 
   Size:        220     512
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     186     732 
   Size:        186     546
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     351     521     530     732 
   Size:        113     238     170       9     202

  Fragments arranged by size: 

                 238     202     170     113       9
 
BsaAI yAC'GTr

Cuts at:      0      49     732 
   Size:         49     683
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     311     341     519     549     732 
   Size:        311      30     178      30     183

  Fragments arranged by size: 

                 311     183     178      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     475     732 
   Size:        475     257
 
BsmI GAATG_Cn'

Cuts at:      0     661     732 
   Size:        661      71
 
BsrGI T'GTAC_A

Cuts at:      0     650     732 
   Size:        650      82
 
BssSI C'ACGA_G

Cuts at:      0     296     732 
   Size:        296     436
 
BtgI C'CryG_G

Cuts at:      0     184     732 
   Size:        184     548
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     148     379     732 
   Size:        148     231     353

  Fragments arranged by size: 

                 353     231     148
 
BtsI GCAGTG_nn'

Cuts at:      0     716     732 
   Size:        716      16
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      78     113     732 
   Size:         78      35     619

  Fragments arranged by size: 

                 619      78      35
 
EaeI y'GGCC_r

Cuts at:      0       2     732 
   Size:          2     730
 
EarI CTCTTCn'nnn_

Cuts at:      0     681     732 
   Size:        681      51
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     184     351     384     521     530     732 
   Size:        113      71     167      33     137       9     202

  Fragments arranged by size: 

                 202     167     137     113      71      33       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     594     732 
   Size:        594     138
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     311     343     621     653     732 
   Size:        311      32     278      32      79

  Fragments arranged by size: 

                 311     278      79      32      32
 
HincII GTy'rAC

Cuts at:      0      24     732 
   Size:         24     708
 
HindIII A'AGCT_T

Cuts at:      0     175     546     732 
   Size:        175     371     186

  Fragments arranged by size: 

                 371     186     175
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     675     732 
   Size:        675      57
 
MscI TGG'CCA

Cuts at:      0       4     732 
   Size:          4     728
 
MspA1I CmG'CkG

Cuts at:      0     656     732 
   Size:        656      76
 
PpuMI rG'GwC_Cy

Cuts at:      0     594     732 
   Size:        594     138
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     164     196     732 
   Size:        164      32     536

  Fragments arranged by size: 

                 536     164      32
 
PstI C_TGCA'G

Cuts at:      0      78     732 
   Size:         78     654
 
PvuII CAG'CTG

Cuts at:      0     656     732 
   Size:        656      76
 
SfcI C'TryA_G

Cuts at:      0      53      74     431     732 
   Size:         53      21     357     301

  Fragments arranged by size: 

                 357     301      53      21
 
SnaBI TAC'GTA

Cuts at:      0      49     732 
   Size:         49     683
 
SspI AAT'ATT

Cuts at:      0     725     732 
   Size:        725       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     636     732 
   Size:        636      96
 
TatI w'GTAC_w

Cuts at:      0     650     732 
   Size:        650      82
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     708     732 
   Size:        708      24
 

 Enzymes that do cut:

     AcuI     AvaI     BanI     BmrI     BpmI    BsaAI    BsaXI    BseRI
     BsmI    BsrGI    BssSI     BtgI    BtgZI     BtsI    CspCI     EaeI
     EarI  Eco57MI EcoO109I    Hin4I   HincII  HindIII     MmeI     MscI
   MspA1I    PpuMI     PsrI     PstI    PvuII     SfcI    SnaBI     SspI
    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     ClaI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MfeI     MluI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI     SrfI     StuI
     StyI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI