Restriction map for
pF1KB8592
(Linear) (Six-base) MAPSORT of: KIBB8592.seq Check: 2068 from: 1 to: 2367
KIBB8592.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2301 2367
Size: 2301 66
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 1309 1454 1763 2367
Size: 641 668 145 309 604
Fragments arranged by size:
668 641 604 309 145
AflIII A'CryG_T
Cuts at: 0 191 416 2244 2367
Size: 191 225 1828 123
Fragments arranged by size:
1828 225 191 123
ApoI r'AATT_y
Cuts at: 0 292 329 408 915 1354 1815 2233 2358
Size: 292 37 79 507 439 461 418 125
Cuts at: 2358 2367
Size: 9
Fragments arranged by size:
507 461 439 418 292 125 79 37
9
BbsI GAAGACnn'nnnn_
Cuts at: 0 826 1315 1419 2015 2367
Size: 826 489 104 596 352
Fragments arranged by size:
826 596 489 352 104
BclI T'GATC_A
Cuts at: 0 2223 2343 2367
Size: 2223 120 24
Fragments arranged by size:
2223 120 24
BfrBI ATG'CAT
Cuts at: 0 1918 2367
Size: 1918 449
BglII A'GATC_T
Cuts at: 0 1308 2367
Size: 1308 1059
Bme1580I G_kGCm'C
Cuts at: 0 2272 2367
Size: 2272 95
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 194 2367
Size: 194 2173
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 2367
Size: 356 2011
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 721 760 1014 2367
Size: 469 252 39 254 1353
Fragments arranged by size:
1353 469 254 252 39
BsaAI yAC'GTr
Cuts at: 0 2134 2367
Size: 2134 233
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 99 129 2367
Size: 99 30 2238
Fragments arranged by size:
2238 99 30
BseYI C'CCAG_C
Cuts at: 0 1485 2367
Size: 1485 882
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1163 2298 2350 2367
Size: 1163 1135 52 17
Fragments arranged by size:
1163 1135 52 17
BsiWI C'GTAC_G
Cuts at: 0 1146 2367
Size: 1146 1221
BsmI GAATG_Cn'
Cuts at: 0 256 1369 2367
Size: 256 1113 998
Fragments arranged by size:
1113 998 256
Bsp1286I G_dGCh'C
Cuts at: 0 2272 2367
Size: 2272 95
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1178 2301 2367
Size: 1178 1123 66
Fragments arranged by size:
1178 1123 66
BsrDI GCAATG_nn'
Cuts at: 0 22 2367
Size: 22 2345
BssSI C'ACGA_G
Cuts at: 0 179 2367
Size: 179 2188
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1948 2367
Size: 1948 419
BstEII G'GTnAC_C
Cuts at: 0 426 2367
Size: 426 1941
BstXI CCAn_nnnn'nTGG
Cuts at: 0 143 2367
Size: 143 2224
BstYI r'GATC_y
Cuts at: 0 1308 2367
Size: 1308 1059
BtsI GCAGTG_nn'
Cuts at: 0 1229 2367
Size: 1229 1138
DraI TTT'AAA
Cuts at: 0 764 911 1055 2367
Size: 764 147 144 1312
Fragments arranged by size:
1312 764 147 144
DrdI GACnn_nn'nnGTC
Cuts at: 0 2017 2367
Size: 2017 350
EaeI y'GGCC_r
Cuts at: 0 65 2367
Size: 65 2302
EarI CTCTTCn'nnn_
Cuts at: 0 374 647 658 953 1290 2367
Size: 374 273 11 295 337 1077
Fragments arranged by size:
1077 374 337 295 273 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 641 1309 1454 1763 2367
Size: 356 285 668 145 309 604
Fragments arranged by size:
668 604 356 309 285 145
EcoRI G'AATT_C
Cuts at: 0 1815 2367
Size: 1815 552
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 365 403 460 2367
Size: 365 38 57 1907
Fragments arranged by size:
1907 365 57 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 224 256 667 699 700 732 2367
Size: 224 32 411 32 1 32 1635
Fragments arranged by size:
1635 411 224 32 32 32 1
HincII GTy'rAC
Cuts at: 0 1143 2125 2367
Size: 1143 982 242
Fragments arranged by size:
1143 982 242
HindIII A'AGCT_T
Cuts at: 0 633 886 2367
Size: 633 253 1481
Fragments arranged by size:
1481 633 253
MfeI C'AATT_G
Cuts at: 0 243 734 2367
Size: 243 491 1633
Fragments arranged by size:
1633 491 243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 144 2367
Size: 59 85 2223
Fragments arranged by size:
2223 85 59
MscI TGG'CCA
Cuts at: 0 67 2367
Size: 67 2300
MslI CAynn'nnrTG
Cuts at: 0 323 421 2132 2367
Size: 323 98 1711 235
Fragments arranged by size:
1711 323 235 98
NsiI A_TGCA'T
Cuts at: 0 1920 2367
Size: 1920 447
NspI r_CATG'y
Cuts at: 0 420 1847 2248 2329 2367
Size: 420 1427 401 81 38
Fragments arranged by size:
1427 420 401 81 38
PciI A'CATG_T
Cuts at: 0 416 2244 2367
Size: 416 1828 123
Fragments arranged by size:
1828 416 123
PsiI TTA'TAA
Cuts at: 0 848 1346 1760 2367
Size: 848 498 414 607
Fragments arranged by size:
848 607 498 414
PstI C_TGCA'G
Cuts at: 0 977 1338 1533 2026 2367
Size: 977 361 195 493 341
Fragments arranged by size:
977 493 361 341 195
SapI GCTCTTCn'nnn_
Cuts at: 0 374 2367
Size: 374 1993
ScaI AGT'ACT
Cuts at: 0 174 2367
Size: 174 2193
SfcI C'TryA_G
Cuts at: 0 973 1334 1505 1529 2022 2367
Size: 973 361 171 24 493 345
Fragments arranged by size:
973 493 361 345 171 24
SmlI C'TyrA_G
Cuts at: 0 448 700 775 1029 2367
Size: 448 252 75 254 1338
Fragments arranged by size:
1338 448 254 252 75
StuI AGG'CCT
Cuts at: 0 443 2367
Size: 443 1924
StyI C'CwwG_G
Cuts at: 0 261 2367
Size: 261 2106
TatI w'GTAC_w
Cuts at: 0 172 249 343 1746 2367
Size: 172 77 94 1403 621
Fragments arranged by size:
1403 621 172 94 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 99 131 2367
Size: 99 32 2236
Fragments arranged by size:
2236 99 32
XbaI T'CTAG_A
Cuts at: 0 1779 2367
Size: 1779 588
XmnI GAAnn'nnTTC
Cuts at: 0 667 1085 2367
Size: 667 418 1282
Fragments arranged by size:
1282 667 418
Enzymes that do cut:
AarI AcuI AflIII ApoI BbsI BclI BfrBI BglII
Bme1580I BplI BpmI BpuEI BsaAI BsaXI BseYI BsgI
BsiWI BsmI Bsp1286I BspMI BsrDI BssSI BstAPI BstEII
BstXI BstYI BtsI DraI DrdI EaeI EarI Eco57MI
EcoRI FalI Hin4I HincII HindIII MfeI MmeI MscI
MslI NsiI NspI PciI PsiI PstI SapI ScaI
SfcI SmlI StuI StyI TatI TstI XbaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BglI BlpI BmgBI BmrI BmtI
Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BsiEI BsiHKAI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII EagI
EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI
HaeII HpaI KasI KpnI MluI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XcmI XhoI XmaI ZraI