Restriction map for
pF1KB8591
(Linear) (Six-base) MAPSORT of: KIBB8591.seq Check: 1459 from: 1 to: 2310
KIBB8591.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1056 2183 2310
Size: 1056 1127 127
Fragments arranged by size:
1127 1056 127
AflIII A'CryG_T
Cuts at: 0 2290 2310
Size: 2290 20
AhdI GACnn_n'nnGTC
Cuts at: 0 1108 1659 2310
Size: 1108 551 651
Fragments arranged by size:
1108 651 551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 950 2310
Size: 950 1360
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 117 149 2310
Size: 117 32 2161
Fragments arranged by size:
2161 117 32
AlwNI CAG_nnn'CTG
Cuts at: 0 538 898 2310
Size: 538 360 1412
Fragments arranged by size:
1412 538 360
ApaI G_GGCC'C
Cuts at: 0 1968 2310
Size: 1968 342
AvaI C'yCGr_G
Cuts at: 0 27 34 242 764 829 2310
Size: 27 7 208 522 65 1481
Fragments arranged by size:
1481 522 208 65 27 7
BamHI G'GATC_C
Cuts at: 0 210 2310
Size: 210 2100
BanI G'GyrC_C
Cuts at: 0 117 238 421 1793 2310
Size: 117 121 183 1372 517
Fragments arranged by size:
1372 517 183 121 117
BanII G_rGCy'C
Cuts at: 0 703 1410 1968 2112 2280 2310
Size: 703 707 558 144 168 30
Fragments arranged by size:
707 703 558 168 144 30
BbeI G_GCGC'C
Cuts at: 0 242 2310
Size: 242 2068
BbsI GAAGACnn'nnnn_
Cuts at: 0 847 2310
Size: 847 1463
BciVI GTATCCnnnnn_n'
Cuts at: 0 105 2310
Size: 105 2205
BglI GCCn_nnn'nGGC
Cuts at: 0 660 2310
Size: 660 1650
BglII A'GATC_T
Cuts at: 0 1428 2310
Size: 1428 882
BlpI GC'TnA_GC
Cuts at: 0 82 524 1409 1414 1770 2310
Size: 82 442 885 5 356 540
Fragments arranged by size:
885 540 442 356 82 5
Bme1580I G_kGCm'C
Cuts at: 0 122 1130 1968 2310
Size: 122 1008 838 342
Fragments arranged by size:
1008 838 342 122
BmrI ACTGGGnnnn_n'
Cuts at: 0 990 1576 1796 2282 2310
Size: 990 586 220 486 28
Fragments arranged by size:
990 586 486 220 28
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 168 1429 2310
Size: 168 1261 881
Fragments arranged by size:
1261 881 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 900 1163 1531 1614 1718 1861 2310
Size: 832 68 263 368 83 104 143 449
Fragments arranged by size:
832 449 368 263 143 104 83 68
Bpu10I CC'TnA_GC
Cuts at: 0 899 950 2310
Size: 899 51 1360
Fragments arranged by size:
1360 899 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 717 2310
Size: 508 209 1593
Fragments arranged by size:
1593 508 209
BsaI GGTCTCn'nnnn_
Cuts at: 0 279 2310
Size: 279 2031
BsaHI Gr'CG_yC
Cuts at: 0 239 2046 2310
Size: 239 1807 264
Fragments arranged by size:
1807 264 239
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2134 2164 2262 2292 2310
Size: 2134 30 98 30 18
Fragments arranged by size:
2134 98 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 400 1665 2270 2310
Size: 400 1265 605 40
Fragments arranged by size:
1265 605 400 40
BseYI C'CCAG_C
Cuts at: 0 547 672 702 1130 2253 2310
Size: 547 125 30 428 1123 57
Fragments arranged by size:
1123 547 428 125 57 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1799 2310
Size: 1799 511
BsiEI CG_ry'CG
Cuts at: 0 102 2310
Size: 102 2208
BsiHKAI G_wGCw'C
Cuts at: 0 1358 1410 1637 2310
Size: 1358 52 227 673
Fragments arranged by size:
1358 673 227 52
BsmBI CGTCTCn'nnnn_
Cuts at: 0 722 1549 2310
Size: 722 827 761
Fragments arranged by size:
827 761 722
Bsp1286I G_dGCh'C
Cuts at: 0 122 703 1130 1358 1410 1637 1968 2112
Size: 122 581 427 228 52 227 331 144
Cuts at: 2112 2280 2310
Size: 168 30
Fragments arranged by size:
581 427 331 228 227 168 144 122
52 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 465 890 2310
Size: 465 425 1420
Fragments arranged by size:
1420 465 425
BsrBI CCG'CTC
Cuts at: 0 86 113 2172 2310
Size: 86 27 2059 138
Fragments arranged by size:
2059 138 86 27
BsrDI GCAATG_nn'
Cuts at: 0 389 2310
Size: 389 1921
BsrFI r'CCGG_y
Cuts at: 0 3 2310
Size: 3 2307
BstEII G'GTnAC_C
Cuts at: 0 1323 1851 2310
Size: 1323 528 459
Fragments arranged by size:
1323 528 459
BstXI CCAn_nnnn'nTGG
Cuts at: 0 615 710 1804 2310
Size: 615 95 1094 506
Fragments arranged by size:
1094 615 506 95
BstYI r'GATC_y
Cuts at: 0 210 1428 2310
Size: 210 1218 882
Fragments arranged by size:
1218 882 210
Bsu36I CC'TnA_GG
Cuts at: 0 314 2310
Size: 314 1996
BtgI C'CryG_G
Cuts at: 0 689 799 1727 1865 2310
Size: 689 110 928 138 445
Fragments arranged by size:
928 689 445 138 110
BtsI GCAGTG_nn'
Cuts at: 0 620 1400 2208 2310
Size: 620 780 808 102
Fragments arranged by size:
808 780 620 102
DraIII CAC_nnn'GTG
Cuts at: 0 1323 1974 2310
Size: 1323 651 336
Fragments arranged by size:
1323 651 336
EaeI y'GGCC_r
Cuts at: 0 99 495 906 1266 1539 2189 2310
Size: 99 396 411 360 273 650 121
Fragments arranged by size:
650 411 396 360 273 121 99
EagI C'GGCC_G
Cuts at: 0 99 2310
Size: 99 2211
EcoICRI GAG'CTC
Cuts at: 0 1408 2310
Size: 1408 902
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 900 1056 1163 1531 1614 1718 1861
Size: 832 68 156 107 368 83 104 143
Cuts at: 1861 2183 2310
Size: 322 127
Fragments arranged by size:
832 368 322 156 143 127 107 104
83 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1573 2310
Size: 1573 737
EcoO109I rG'GnC_Cy
Cuts at: 0 31 555 825 895 1578 2310
Size: 31 524 270 70 683 732
Fragments arranged by size:
732 683 524 270 70 31
FspI TGC'GCA
Cuts at: 0 1742 2310
Size: 1742 568
HaeII r_GCGC'y
Cuts at: 0 242 1874 2310
Size: 242 1632 436
Fragments arranged by size:
1632 436 242
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 202 234 1094 1126 1136 1168 1392 1424
Size: 202 32 860 32 10 32 224 32
Cuts at: 1424 1631 1645 1660 1663 1677 1692 2134 2166
Size: 207 14 15 3 14 15 442 32
Cuts at: 2166 2287 2310
Size: 121 23
Fragments arranged by size:
860 442 224 207 202 121 32 32
32 32 32 23 15 15 14 14
10 3
KasI G'GCGC_C
Cuts at: 0 238 2310
Size: 238 2072
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1059 2310
Size: 1059 1251
MscI TGG'CCA
Cuts at: 0 1268 2191 2310
Size: 1268 923 119
Fragments arranged by size:
1268 923 119
MspA1I CmG'CkG
Cuts at: 0 528 538 1261 1484 1738 2181 2310
Size: 528 10 723 223 254 443 129
Fragments arranged by size:
723 528 443 254 223 129 10
NaeI GCC'GGC
Cuts at: 0 5 2310
Size: 5 2305
NarI GG'CG_CC
Cuts at: 0 239 2310
Size: 239 2071
NcoI C'CATG_G
Cuts at: 0 1865 2310
Size: 1865 445
NgoMIV G'CCGG_C
Cuts at: 0 3 2310
Size: 3 2307
NspI r_CATG'y
Cuts at: 0 1747 2294 2310
Size: 1747 547 16
Fragments arranged by size:
1747 547 16
PasI CC'CwG_GG
Cuts at: 0 1858 2310
Size: 1858 452
PciI A'CATG_T
Cuts at: 0 2290 2310
Size: 2290 20
PflMI CCAn_nnn'nTGG
Cuts at: 0 1264 1974 2119 2310
Size: 1264 710 145 191
Fragments arranged by size:
1264 710 191 145
PfoI T'CCnGG_A
Cuts at: 0 1223 2310
Size: 1223 1087
PpuMI rG'GwC_Cy
Cuts at: 0 31 555 825 1578 2310
Size: 31 524 270 753 732
Fragments arranged by size:
753 732 524 270 31
PshAI GACnn'nnGTC
Cuts at: 0 155 362 2310
Size: 155 207 1948
Fragments arranged by size:
1948 207 155
PspOMI G'GGCC_C
Cuts at: 0 1964 2310
Size: 1964 346
PstI C_TGCA'G
Cuts at: 0 929 2310
Size: 929 1381
PvuII CAG'CTG
Cuts at: 0 538 1261 1484 1738 2310
Size: 538 723 223 254 572
Fragments arranged by size:
723 572 538 254 223
SacI G_AGCT'C
Cuts at: 0 1410 2310
Size: 1410 900
SanDI GG'GwC_CC
Cuts at: 0 31 2310
Size: 31 2279
SbfI CC_TGCA'GG
Cuts at: 0 929 2310
Size: 929 1381
SfcI C'TryA_G
Cuts at: 0 44 333 925 1286 1733 2133 2310
Size: 44 289 592 361 447 400 177
Fragments arranged by size:
592 447 400 361 289 177 44
SfoI GGC'GCC
Cuts at: 0 240 2310
Size: 240 2070
SmaI CCC'GGG
Cuts at: 0 29 36 244 2310
Size: 29 7 208 2066
Fragments arranged by size:
2066 208 29 7
SmlI C'TyrA_G
Cuts at: 0 487 696 2310
Size: 487 209 1614
Fragments arranged by size:
1614 487 209
StyI C'CwwG_G
Cuts at: 0 1865 2310
Size: 1865 445
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1247 2310
Size: 1247 1063
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1829 1861 2213 2245 2310
Size: 1829 32 352 32 65
Fragments arranged by size:
1829 352 65 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1582 2310
Size: 1582 728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1138 1589 1772 2310
Size: 1138 451 183 538
Fragments arranged by size:
1138 538 451 183
XmaI C'CCGG_G
Cuts at: 0 27 34 242 2310
Size: 27 7 208 2068
Fragments arranged by size:
2068 208 27 7
Enzymes that do cut:
AcuI AflIII AhdI AlfI AloI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BciVI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BstEII BstXI BstYI
Bsu36I BtgI BtsI DraIII EaeI EagI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I KasI MmeI MscI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI
SanDI SbfI SfcI SfoI SmaI SmlI StyI TaqII
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbvCI BcgI BclI BfrBI BmgBI BmtI BsaAI BsaBI
BsaWI BsiWI BsmI BspEI BspHI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI
EarI EciI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI MslI NdeI NheI
NotI NruI NsiI PacI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacII SalI SapI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI XbaI XhoI XmnI ZraI