Restriction map for
pF1KB8590
(Linear) (Six-base) MAPSORT of: KIBB8590.seq Check: 7125 from: 1 to: 1035
KIBB8590.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
ApaLI G'TGCA_C
Cuts at: 0 734 1035
Size: 734 301
ApoI r'AATT_y
Cuts at: 0 87 368 1035
Size: 87 281 667
Fragments arranged by size:
667 281 87
BamHI G'GATC_C
Cuts at: 0 155 1035
Size: 155 880
BanI G'GyrC_C
Cuts at: 0 814 1035
Size: 814 221
BanII G_rGCy'C
Cuts at: 0 556 1035
Size: 556 479
BciVI GTATCCnnnnn_n'
Cuts at: 0 966 1035
Size: 966 69
BclI T'GATC_A
Cuts at: 0 231 1035
Size: 231 804
Bme1580I G_kGCm'C
Cuts at: 0 738 1035
Size: 738 297
BmrI ACTGGGnnnn_n'
Cuts at: 0 704 1035
Size: 704 331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 1035
Size: 278 757
BsaI GGTCTCn'nnnn_
Cuts at: 0 763 1035
Size: 763 272
BsaWI w'CCGG_w
Cuts at: 0 26 1035
Size: 26 1009
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 390 420 1035
Size: 390 30 615
Fragments arranged by size:
615 390 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 605 1035
Size: 605 430
BseYI C'CCAG_C
Cuts at: 0 54 1035
Size: 54 981
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 923 1035
Size: 623 300 112
Fragments arranged by size:
623 300 112
BsiHKAI G_wGCw'C
Cuts at: 0 738 1035
Size: 738 297
BsmI GAATG_Cn'
Cuts at: 0 527 1035
Size: 527 508
Bsp1286I G_dGCh'C
Cuts at: 0 556 738 1035
Size: 556 182 297
Fragments arranged by size:
556 297 182
BspEI T'CCGG_A
Cuts at: 0 26 1035
Size: 26 1009
BspHI T'CATG_A
Cuts at: 0 452 461 1035
Size: 452 9 574
Fragments arranged by size:
574 452 9
BsrDI GCAATG_nn'
Cuts at: 0 842 1035
Size: 842 193
BstYI r'GATC_y
Cuts at: 0 155 781 1035
Size: 155 626 254
Fragments arranged by size:
626 254 155
BtgI C'CryG_G
Cuts at: 0 506 806 1035
Size: 506 300 229
Fragments arranged by size:
506 300 229
BtsI GCAGTG_nn'
Cuts at: 0 611 1035
Size: 611 424
ClaI AT'CG_AT
Cuts at: 0 208 1035
Size: 208 827
DraI TTT'AAA
Cuts at: 0 225 1035
Size: 225 810
EaeI y'GGCC_r
Cuts at: 0 174 695 1035
Size: 174 521 340
Fragments arranged by size:
521 340 174
EarI CTCTTCn'nnn_
Cuts at: 0 644 1035
Size: 644 391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 1035
Size: 278 757
EcoO109I rG'GnC_Cy
Cuts at: 0 634 1035
Size: 634 401
EcoRI G'AATT_C
Cuts at: 0 87 368 1035
Size: 87 281 667
Fragments arranged by size:
667 281 87
HincII GTy'rAC
Cuts at: 0 116 1035
Size: 116 919
HpaI GTT'AAC
Cuts at: 0 116 1035
Size: 116 919
MfeI C'AATT_G
Cuts at: 0 485 1035
Size: 485 550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 1035
Size: 492 543
MscI TGG'CCA
Cuts at: 0 176 697 1035
Size: 176 521 338
Fragments arranged by size:
521 338 176
MslI CAynn'nnrTG
Cuts at: 0 382 590 1035
Size: 382 208 445
Fragments arranged by size:
445 382 208
MspA1I CmG'CkG
Cuts at: 0 667 1035
Size: 667 368
NcoI C'CATG_G
Cuts at: 0 506 1035
Size: 506 529
NdeI CA'TA_TG
Cuts at: 0 543 1035
Size: 543 492
NspI r_CATG'y
Cuts at: 0 867 1035
Size: 867 168
PasI CC'CwG_GG
Cuts at: 0 741 1035
Size: 741 294
PflMI CCAn_nnn'nTGG
Cuts at: 0 922 1035
Size: 922 113
PpuMI rG'GwC_Cy
Cuts at: 0 634 1035
Size: 634 401
PvuII CAG'CTG
Cuts at: 0 667 1035
Size: 667 368
SspI AAT'ATT
Cuts at: 0 626 1035
Size: 626 409
StuI AGG'CCT
Cuts at: 0 70 683 1035
Size: 70 613 352
Fragments arranged by size:
613 352 70
StyI C'CwwG_G
Cuts at: 0 65 506 1035
Size: 65 441 529
Fragments arranged by size:
529 441 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 68 468 589 721 1035
Size: 68 400 121 132 314
Fragments arranged by size:
400 314 132 121 68
TatI w'GTAC_w
Cuts at: 0 386 1035
Size: 386 649
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 147 179 1035
Size: 147 32 856
Fragments arranged by size:
856 147 32
XmnI GAAnn'nnTTC
Cuts at: 0 372 719 1035
Size: 372 347 316
Fragments arranged by size:
372 347 316
Enzymes that do cut:
ApaLI ApoI BamHI BanI BanII BciVI BclI Bme1580I
BmrI BpmI BsaI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI BstYI BtgI
BtsI ClaI DraI EaeI EarI Eco57MI EcoO109I EcoRI
HincII HpaI MfeI MmeI MscI MslI MspA1I NcoI
NdeI NspI PasI PflMI PpuMI PvuII SspI StuI
StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
AscI AseI AsiSI AvaI AvrII BaeI BbeI BbsI
BbvCI BcgI BfrBI BglI BglII BlpI BmgBI BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI
BsmBI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI CspCI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HindIII KasI KpnI MluI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SwaI Tth111I XbaI XcmI XhoI XmaI ZraI