Restriction map for
pF1KB8588
(Linear) (Six-base) MAPSORT of: KIBB8588.seq Check: 9225 from: 1 to: 3588
KIBB8588.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AccI GT'mk_AC
Cuts at: 0 1715 3588
Size: 1715 1873
AclI AA'CG_TT
Cuts at: 0 2439 3588
Size: 2439 1149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 140 289 611 785 803 1642 2282
Size: 118 22 149 322 174 18 839 640
Cuts at: 2282 3441 3588
Size: 1159 147
Fragments arranged by size:
1159 839 640 322 174 149 147 118
22 18
AflII C'TTAA_G
Cuts at: 0 58 1063 1356 1435 3588
Size: 58 1005 293 79 2153
Fragments arranged by size:
2153 1005 293 79 58
AleI CACnn'nnGTG
Cuts at: 0 2962 3588
Size: 2962 626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 594 3588
Size: 594 2994
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1598 1630 3197 3229 3588
Size: 1598 32 1567 32 359
Fragments arranged by size:
1598 1567 359 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1726 3588
Size: 1726 1862
ApaLI G'TGCA_C
Cuts at: 0 2350 3588
Size: 2350 1238
ApoI r'AATT_y
Cuts at: 0 1100 1284 1919 2110 2399 2893 3588
Size: 1100 184 635 191 289 494 695
Fragments arranged by size:
1100 695 635 494 289 191 184
AseI AT'TA_AT
Cuts at: 0 2330 3588
Size: 2330 1258
BanI G'GyrC_C
Cuts at: 0 396 3588
Size: 396 3192
BbsI GAAGACnn'nnnn_
Cuts at: 0 362 720 875 925 3263 3588
Size: 362 358 155 50 2338 325
Fragments arranged by size:
2338 362 358 325 155 50
BciVI GTATCCnnnnn_n'
Cuts at: 0 1798 3528 3588
Size: 1798 1730 60
Fragments arranged by size:
1798 1730 60
BclI T'GATC_A
Cuts at: 0 495 1995 3588
Size: 495 1500 1593
Fragments arranged by size:
1593 1500 495
Bme1580I G_kGCm'C
Cuts at: 0 401 2354 3588
Size: 401 1953 1234
Fragments arranged by size:
1953 1234 401
BmrI ACTGGGnnnn_n'
Cuts at: 0 396 3588
Size: 396 3192
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1725 3588
Size: 1725 1863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3395 3588
Size: 3395 193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3056 3588
Size: 3056 532
BsaAI yAC'GTr
Cuts at: 0 1593 3588
Size: 1593 1995
BsaBI GATnn'nnATC
Cuts at: 0 827 1898 3588
Size: 827 1071 1690
Fragments arranged by size:
1690 1071 827
BsaWI w'CCGG_w
Cuts at: 0 426 2486 3588
Size: 426 2060 1102
Fragments arranged by size:
2060 1102 426
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2538 2568 2615 2645 3588
Size: 2538 30 47 30 943
Fragments arranged by size:
2538 943 47 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3407 3588
Size: 3407 181
BseYI C'CCAG_C
Cuts at: 0 1214 2768 3588
Size: 1214 1554 820
Fragments arranged by size:
1554 1214 820
BsiHKAI G_wGCw'C
Cuts at: 0 280 2354 3588
Size: 280 2074 1234
Fragments arranged by size:
2074 1234 280
BsmI GAATG_Cn'
Cuts at: 0 2707 3588
Size: 2707 881
Bsp1286I G_dGCh'C
Cuts at: 0 280 401 2354 3588
Size: 280 121 1953 1234
Fragments arranged by size:
1953 1234 280 121
BspEI T'CCGG_A
Cuts at: 0 2486 3588
Size: 2486 1102
BspHI T'CATG_A
Cuts at: 0 969 1937 3588
Size: 969 968 1651
Fragments arranged by size:
1651 969 968
BsrBI CCG'CTC
Cuts at: 0 1643 3070 3588
Size: 1643 1427 518
Fragments arranged by size:
1643 1427 518
BsrGI T'GTAC_A
Cuts at: 0 2290 3588
Size: 2290 1298
BssSI C'ACGA_G
Cuts at: 0 279 3588
Size: 279 3309
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1882 2713 3588
Size: 1882 831 875
Fragments arranged by size:
1882 875 831
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1405 3588
Size: 1405 2183
BstYI r'GATC_y
Cuts at: 0 2481 3588
Size: 2481 1107
Bsu36I CC'TnA_GG
Cuts at: 0 3383 3588
Size: 3383 205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3202 3588
Size: 3202 386
BtsI GCAGTG_nn'
Cuts at: 0 699 2027 3588
Size: 699 1328 1561
Fragments arranged by size:
1561 1328 699
ClaI AT'CG_AT
Cuts at: 0 3195 3588
Size: 3195 393
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3149 3184 3588
Size: 3149 35 404
Fragments arranged by size:
3149 404 35
DraI TTT'AAA
Cuts at: 0 1230 3588
Size: 1230 2358
EaeI y'GGCC_r
Cuts at: 0 1953 3588
Size: 1953 1635
EarI CTCTTCn'nnn_
Cuts at: 0 268 522 527 542 1661 1730 1810 3588
Size: 268 254 5 15 1119 69 80 1778
Fragments arranged by size:
1778 1119 268 254 80 69 15 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 140 289 611 785 803 1642 2282
Size: 118 22 149 322 174 18 839 640
Cuts at: 2282 3395 3441 3588
Size: 1113 46 147
Fragments arranged by size:
1113 839 640 322 174 149 147 118
46 22 18
EcoRI G'AATT_C
Cuts at: 0 2399 3588
Size: 2399 1189
EcoRV GAT'ATC
Cuts at: 0 1900 3588
Size: 1900 1688
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3149 3588
Size: 3149 439
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3069 3101 3215 3247 3588
Size: 3069 32 114 32 341
Fragments arranged by size:
3069 341 114 32 32
HincII GTy'rAC
Cuts at: 0 407 647 2991 3588
Size: 407 240 2344 597
Fragments arranged by size:
2344 597 407 240
HindIII A'AGCT_T
Cuts at: 0 1438 3588
Size: 1438 2150
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2235 3588
Size: 2235 1353
MscI TGG'CCA
Cuts at: 0 1955 3588
Size: 1955 1633
MslI CAynn'nnrTG
Cuts at: 0 576 833 1545 2429 2962 3588
Size: 576 257 712 884 533 626
Fragments arranged by size:
884 712 626 576 533 257
MspA1I CmG'CkG
Cuts at: 0 10 2122 3588
Size: 10 2112 1466
Fragments arranged by size:
2112 1466 10
NdeI CA'TA_TG
Cuts at: 0 1221 2273 3588
Size: 1221 1052 1315
Fragments arranged by size:
1315 1221 1052
NspI r_CATG'y
Cuts at: 0 879 3063 3588
Size: 879 2184 525
Fragments arranged by size:
2184 879 525
PasI CC'CwG_GG
Cuts at: 0 1771 3588
Size: 1771 1817
PflMI CCAn_nnn'nTGG
Cuts at: 0 2039 2818 3588
Size: 2039 779 770
Fragments arranged by size:
2039 779 770
PshAI GACnn'nnGTC
Cuts at: 0 1065 3588
Size: 1065 2523
PvuII CAG'CTG
Cuts at: 0 10 2122 3588
Size: 10 2112 1466
Fragments arranged by size:
2112 1466 10
SapI GCTCTTCn'nnn_
Cuts at: 0 268 1661 3588
Size: 268 1393 1927
Fragments arranged by size:
1927 1393 268
ScaI AGT'ACT
Cuts at: 0 2466 2547 3588
Size: 2466 81 1041
Fragments arranged by size:
2466 1041 81
SfcI C'TryA_G
Cuts at: 0 5 3588
Size: 5 3583
SmlI C'TyrA_G
Cuts at: 0 58 1063 1356 1435 3071 3588
Size: 58 1005 293 79 1636 517
Fragments arranged by size:
1636 1005 517 293 79 58
SnaBI TAC'GTA
Cuts at: 0 1593 3588
Size: 1593 1995
SphI G_CATG'C
Cuts at: 0 879 3588
Size: 879 2709
SspI AAT'ATT
Cuts at: 0 1868 3588
Size: 1868 1720
StyI C'CwwG_G
Cuts at: 0 3519 3588
Size: 3519 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1103 2679 3588
Size: 1103 1576 909
Fragments arranged by size:
1576 1103 909
TatI w'GTAC_w
Cuts at: 0 748 2290 2464 2545 3588
Size: 748 1542 174 81 1043
Fragments arranged by size:
1542 1043 748 174 81
Tth111I GACn'n_nGTC
Cuts at: 0 1027 3588
Size: 1027 2561
XmnI GAAnn'nnTTC
Cuts at: 0 1286 3588
Size: 1286 2302
Enzymes that do cut:
AccI AclI AcuI AflII AleI AlfI AloI AlwNI
ApaLI ApoI AseI BanI BbsI BciVI BclI Bme1580I
BmrI BplI BpmI BpuEI BsaAI BsaBI BsaWI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI
BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgZI BtsI
ClaI CspCI DraI EaeI EarI Eco57MI EcoRI EcoRV
FalI Hin4I HincII HindIII MmeI MscI MslI MspA1I
NdeI NspI PasI PflMI PshAI PvuII SapI ScaI
SfcI SmlI SnaBI SphI SspI StyI TaqII TatI
Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflIII AgeI AhdI ApaI
AscI AsiSI AvaI AvrII BaeI BamHI BanII BbeI
BbvCI BcgI BfrBI BglI BglII BlpI BmgBI BmtI
Bpu10I BsaI BsaHI BsgI BsiEI BsiWI BsmBI BspMI
BsrDI BsrFI BssHII BstBI BstEII BstZ17I BtgI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SpeI SrfI StuI SwaI TstI XbaI XcmI XhoI
XmaI ZraI