Restriction map for
pF1KB8585
(Linear) (Six-base) MAPSORT of: KIBB8585.seq Check: 8982 from: 1 to: 2388
KIBB8585.orf
With 182 enzymes: SgfI *
November 4, 2009 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 981 2388
Size: 981 1407
AccI GT'mk_AC
Cuts at: 0 236 2388
Size: 236 2152
AclI AA'CG_TT
Cuts at: 0 708 2388
Size: 708 1680
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1013 1905 2388
Size: 445 568 892 483
Fragments arranged by size:
892 568 483 445
AflII C'TTAA_G
Cuts at: 0 1740 2388
Size: 1740 648
AhdI GACnn_n'nnGTC
Cuts at: 0 1290 2388
Size: 1290 1098
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2142 2388
Size: 2142 246
ApaLI G'TGCA_C
Cuts at: 0 2018 2388
Size: 2018 370
ApoI r'AATT_y
Cuts at: 0 650 666 817 1383 1635 1813 2165 2388
Size: 650 16 151 566 252 178 352 223
Fragments arranged by size:
650 566 352 252 223 178 151 16
AseI AT'TA_AT
Cuts at: 0 1064 2388
Size: 1064 1324
BbsI GAAGACnn'nnnn_
Cuts at: 0 1436 2261 2388
Size: 1436 825 127
Fragments arranged by size:
1436 825 127
BciVI GTATCCnnnnn_n'
Cuts at: 0 1401 2388
Size: 1401 987
BclI T'GATC_A
Cuts at: 0 332 1712 1889 1992 2388
Size: 332 1380 177 103 396
Fragments arranged by size:
1380 396 332 177 103
BglI GCCn_nnn'nGGC
Cuts at: 0 61 1472 2388
Size: 61 1411 916
Fragments arranged by size:
1411 916 61
BglII A'GATC_T
Cuts at: 0 276 2388
Size: 276 2112
Bme1580I G_kGCm'C
Cuts at: 0 1962 2022 2388
Size: 1962 60 366
Fragments arranged by size:
1962 366 60
BmtI G_CTAG'C
Cuts at: 0 1876 2388
Size: 1876 512
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2275 2388
Size: 2275 113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 2341 2388
Size: 811 1530 47
Fragments arranged by size:
1530 811 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 758 1343 2388
Size: 450 308 585 1045
Fragments arranged by size:
1045 585 450 308
BsaI GGTCTCn'nnnn_
Cuts at: 0 2383 2388
Size: 2383 5
BsaWI w'CCGG_w
Cuts at: 0 158 2388
Size: 158 2230
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2077 2388
Size: 2077 311
BseYI C'CCAG_C
Cuts at: 0 954 1771 2388
Size: 954 817 617
Fragments arranged by size:
954 817 617
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 1196 2170 2388
Size: 205 991 974 218
Fragments arranged by size:
991 974 218 205
BsiHKAI G_wGCw'C
Cuts at: 0 1764 2014 2022 2388
Size: 1764 250 8 366
Fragments arranged by size:
1764 366 250 8
BsmI GAATG_Cn'
Cuts at: 0 593 2388
Size: 593 1795
Bsp1286I G_dGCh'C
Cuts at: 0 1764 1962 2014 2022 2388
Size: 1764 198 52 8 366
Fragments arranged by size:
1764 366 198 52 8
BspEI T'CCGG_A
Cuts at: 0 158 2388
Size: 158 2230
BspHI T'CATG_A
Cuts at: 0 1794 1935 2388
Size: 1794 141 453
Fragments arranged by size:
1794 453 141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 981 2388
Size: 981 1407
BsrDI GCAATG_nn'
Cuts at: 0 896 2388
Size: 896 1492
BsrGI T'GTAC_A
Cuts at: 0 291 2388
Size: 291 2097
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1768 2388
Size: 1768 620
BstBI TT'CG_AA
Cuts at: 0 447 2388
Size: 447 1941
BstYI r'GATC_y
Cuts at: 0 276 2388
Size: 276 2112
Bsu36I CC'TnA_GG
Cuts at: 0 23 2388
Size: 23 2365
BtgI C'CryG_G
Cuts at: 0 411 536 2388
Size: 411 125 1852
Fragments arranged by size:
1852 411 125
BtsI GCAGTG_nn'
Cuts at: 0 1582 2388
Size: 1582 806
DraI TTT'AAA
Cuts at: 0 2228 2388
Size: 2228 160
EaeI y'GGCC_r
Cuts at: 0 203 2388
Size: 203 2185
EarI CTCTTCn'nnn_
Cuts at: 0 85 1922 2032 2388
Size: 85 1837 110 356
Fragments arranged by size:
1837 356 110 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 811 1013 1905 2341 2388
Size: 445 366 202 892 436 47
Fragments arranged by size:
892 445 436 366 202 47
EcoNI CCTnn'n_nnAGG
Cuts at: 0 488 2388
Size: 488 1900
EcoO109I rG'GnC_Cy
Cuts at: 0 1503 2388
Size: 1503 885
EcoRI G'AATT_C
Cuts at: 0 1635 1813 2388
Size: 1635 178 575
Fragments arranged by size:
1635 575 178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2170 2388
Size: 2170 218
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 822 854 935 967 1027 1059 1276 1308
Size: 822 32 81 32 60 32 217 32
Cuts at: 1308 1392 1424 2388
Size: 84 32 964
Fragments arranged by size:
964 822 217 84 81 60 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1218 2388
Size: 1218 1170
HindIII A'AGCT_T
Cuts at: 0 112 2388
Size: 112 2276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 668 2388
Size: 321 347 1720
Fragments arranged by size:
1720 347 321
MscI TGG'CCA
Cuts at: 0 205 2388
Size: 205 2183
MslI CAynn'nnrTG
Cuts at: 0 872 2388
Size: 872 1516
MspA1I CmG'CkG
Cuts at: 0 958 1569 1614 2388
Size: 958 611 45 774
Fragments arranged by size:
958 774 611 45
NcoI C'CATG_G
Cuts at: 0 536 2388
Size: 536 1852
NdeI CA'TA_TG
Cuts at: 0 1809 2388
Size: 1809 579
NheI G'CTAG_C
Cuts at: 0 1872 2388
Size: 1872 516
NspI r_CATG'y
Cuts at: 0 136 1026 2388
Size: 136 890 1362
Fragments arranged by size:
1362 890 136
PfoI T'CCnGG_A
Cuts at: 0 373 2388
Size: 373 2015
PpuMI rG'GwC_Cy
Cuts at: 0 1503 2388
Size: 1503 885
PsiI TTA'TAA
Cuts at: 0 1340 2388
Size: 1340 1048
PvuII CAG'CTG
Cuts at: 0 1614 2388
Size: 1614 774
ScaI AGT'ACT
Cuts at: 0 1279 1519 2388
Size: 1279 240 869
Fragments arranged by size:
1279 869 240
SexAI A'CCwGG_T
Cuts at: 0 1364 2388
Size: 1364 1024
SfcI C'TryA_G
Cuts at: 0 756 998 1424 2388
Size: 756 242 426 964
Fragments arranged by size:
964 756 426 242
SmlI C'TyrA_G
Cuts at: 0 429 773 1358 1740 2388
Size: 429 344 585 382 648
Fragments arranged by size:
648 585 429 382 344
SspI AAT'ATT
Cuts at: 0 1233 2388
Size: 1233 1155
StyI C'CwwG_G
Cuts at: 0 316 536 2388
Size: 316 220 1852
Fragments arranged by size:
1852 316 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 570 1280 2388
Size: 570 710 1108
Fragments arranged by size:
1108 710 570
TatI w'GTAC_w
Cuts at: 0 291 546 1277 1517 2388
Size: 291 255 731 240 871
Fragments arranged by size:
871 731 291 255 240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 254 286 1941 1973 2388
Size: 254 32 1655 32 415
Fragments arranged by size:
1655 415 254 32 32
XbaI T'CTAG_A
Cuts at: 0 1012 1116 2388
Size: 1012 104 1272
Fragments arranged by size:
1272 1012 104
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1383 2388
Size: 1383 1005
XmnI GAAnn'nnTTC
Cuts at: 0 1918 2388
Size: 1918 470
Enzymes that do cut:
AarI AccI AclI AcuI AflII AhdI AlfI ApaLI
ApoI AseI BbsI BciVI BclI BglI BglII Bme1580I
BmtI BplI BpmI BpuEI BsaI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI
BsrGI BstAPI BstBI BstYI Bsu36I BtgI BtsI DraI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
HincII HindIII MmeI MscI MslI MspA1I NcoI NdeI
NheI NspI PfoI PpuMI PsiI PvuII ScaI SexAI
SfcI SmlI SspI StyI TaqII TatI TstI XbaI
XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflIII AgeI AleI AloI AlwNI
ApaI AscI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbvCI BcgI BfrBI BlpI BmgBI BmrI
Bpu10I BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BsmBI
BsrBI BsrFI BssHII BssSI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI NaeI NarI NgoMIV NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI Tth111I XhoI
XmaI ZraI