Restriction map for
pF1KB8581
(Linear) (Six-base) MAPSORT of: KIBB8581.seq Check: 6993 from: 1 to: 1566
KIBB8581.orf
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 90 561 589 1566
Size: 90 471 28 977
Fragments arranged by size:
977 471 90 28
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 750 1566
Size: 750 816
AflIII A'CryG_T
Cuts at: 0 1376 1566
Size: 1376 190
AleI CACnn'nnGTG
Cuts at: 0 442 1455 1566
Size: 442 1013 111
Fragments arranged by size:
1013 442 111
AlwNI CAG_nnn'CTG
Cuts at: 0 922 1566
Size: 922 644
ApaI G_GGCC'C
Cuts at: 0 33 656 1389 1566
Size: 33 623 733 177
Fragments arranged by size:
733 623 177 33
ApoI r'AATT_y
Cuts at: 0 531 876 1566
Size: 531 345 690
Fragments arranged by size:
690 531 345
BamHI G'GATC_C
Cuts at: 0 902 1566
Size: 902 664
BanI G'GyrC_C
Cuts at: 0 549 601 739 777 796 891 1566
Size: 549 52 138 38 19 95 675
Fragments arranged by size:
675 549 138 95 52 38 19
BanII G_rGCy'C
Cuts at: 0 33 138 656 1178 1225 1389 1566
Size: 33 105 518 522 47 164 177
Fragments arranged by size:
522 518 177 164 105 47 33
BbeI G_GCGC'C
Cuts at: 0 743 895 1566
Size: 743 152 671
Fragments arranged by size:
743 671 152
BbsI GAAGACnn'nnnn_
Cuts at: 0 1217 1566
Size: 1217 349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1077 1111 1566
Size: 1077 34 455
Fragments arranged by size:
1077 455 34
BclI T'GATC_A
Cuts at: 0 1007 1566
Size: 1007 559
BglII A'GATC_T
Cuts at: 0 1418 1566
Size: 1418 148
BlpI GC'TnA_GC
Cuts at: 0 522 1219 1566
Size: 522 697 347
Fragments arranged by size:
697 522 347
Bme1580I G_kGCm'C
Cuts at: 0 33 403 656 1389 1566
Size: 33 370 253 733 177
Fragments arranged by size:
733 370 253 177 33
BmgBI CAC'GTC
Cuts at: 0 1379 1566
Size: 1379 187
BmrI ACTGGGnnnn_n'
Cuts at: 0 35 659 1566
Size: 35 624 907
Fragments arranged by size:
907 624 35
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 375 434 485 803 1018 1039 1045
Size: 137 238 59 51 318 215 21 6
Cuts at: 1045 1249 1566
Size: 204 317
Fragments arranged by size:
318 317 238 215 204 137 59 51
21 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 1315 1336 1566
Size: 315 1000 21 230
Fragments arranged by size:
1000 315 230 21
BsaHI Gr'CG_yC
Cuts at: 0 740 892 1566
Size: 740 152 674
Fragments arranged by size:
740 674 152
BsaWI w'CCGG_w
Cuts at: 0 505 1550 1566
Size: 505 1045 16
Fragments arranged by size:
1045 505 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 180 210 1566
Size: 180 30 1356
Fragments arranged by size:
1356 180 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1246 1297 1513 1566
Size: 1246 51 216 53
Fragments arranged by size:
1246 216 53 51
BseYI C'CCAG_C
Cuts at: 0 315 331 345 539 655 751 907 1193
Size: 315 16 14 194 116 96 156 286
Cuts at: 1193 1566
Size: 373
Fragments arranged by size:
373 315 286 194 156 116 96 16
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1108 1566
Size: 1108 458
BsiHKAI G_wGCw'C
Cuts at: 0 382 1178 1370 1566
Size: 382 796 192 196
Fragments arranged by size:
796 382 196 192
Bsp1286I G_dGCh'C
Cuts at: 0 33 138 382 403 656 1178 1225 1370
Size: 33 105 244 21 253 522 47 145
Cuts at: 1370 1389 1566
Size: 19 177
Fragments arranged by size:
522 253 244 177 145 105 47 33
21 19
BspEI T'CCGG_A
Cuts at: 0 1550 1566
Size: 1550 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 90 561 589 1223 1274 1566
Size: 90 471 28 634 51 292
Fragments arranged by size:
634 471 292 90 51 28
BsrBI CCG'CTC
Cuts at: 0 5 934 1044 1253 1566
Size: 5 929 110 209 313
Fragments arranged by size:
929 313 209 110 5
BsrFI r'CCGG_y
Cuts at: 0 169 412 862 1523 1566
Size: 169 243 450 661 43
Fragments arranged by size:
661 450 243 169 43
BstEII G'GTnAC_C
Cuts at: 0 1503 1566
Size: 1503 63
BstXI CCAn_nnnn'nTGG
Cuts at: 0 444 1566
Size: 444 1122
BstYI r'GATC_y
Cuts at: 0 902 1347 1418 1427 1566
Size: 902 445 71 9 139
Fragments arranged by size:
902 445 139 71 9
BtgI C'CryG_G
Cuts at: 0 320 545 1566
Size: 320 225 1021
Fragments arranged by size:
1021 320 225
BtsI GCAGTG_nn'
Cuts at: 0 56 746 1408 1450 1462 1566
Size: 56 690 662 42 12 104
Fragments arranged by size:
690 662 104 56 42 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 399 434 849 884 1566
Size: 399 35 415 35 682
Fragments arranged by size:
682 415 399 35 35
DraI TTT'AAA
Cuts at: 0 881 1566
Size: 881 685
EaeI y'GGCC_r
Cuts at: 0 200 575 1566
Size: 200 375 991
Fragments arranged by size:
991 375 200
EarI CTCTTCn'nnn_
Cuts at: 0 1153 1566
Size: 1153 413
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 966 1566
Size: 966 600
EcoICRI GAG'CTC
Cuts at: 0 1176 1566
Size: 1176 390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 375 434 485 750 803 1018 1039
Size: 137 238 59 51 265 53 215 21
Cuts at: 1039 1045 1249 1566
Size: 6 204 317
Fragments arranged by size:
317 265 238 215 204 137 59 53
51 21 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 86 1057 1566
Size: 86 971 509
Fragments arranged by size:
971 509 86
EcoO109I rG'GnC_Cy
Cuts at: 0 29 30 1385 1386 1566
Size: 29 1 1355 1 180
Fragments arranged by size:
1355 180 29 1 1
EcoRI G'AATT_C
Cuts at: 0 531 1566
Size: 531 1035
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 179 309 1566
Size: 179 130 1257
Fragments arranged by size:
1257 179 130
FspI TGC'GCA
Cuts at: 0 1439 1566
Size: 1439 127
FspAI rTGC'GCAy
Cuts at: 0 1439 1566
Size: 1439 127
HaeII r_GCGC'y
Cuts at: 0 743 895 1566
Size: 743 152 671
Fragments arranged by size:
743 671 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 698 730 1097 1129 1456 1488 1566
Size: 698 32 367 32 327 32 78
Fragments arranged by size:
698 367 327 78 32 32 32
HincII GTy'rAC
Cuts at: 0 1074 1566
Size: 1074 492
KasI G'GCGC_C
Cuts at: 0 739 891 1566
Size: 739 152 675
Fragments arranged by size:
739 675 152
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 709 1566
Size: 168 541 857
Fragments arranged by size:
857 541 168
MslI CAynn'nnrTG
Cuts at: 0 442 1277 1455 1566
Size: 442 835 178 111
Fragments arranged by size:
835 442 178 111
MspA1I CmG'CkG
Cuts at: 0 307 335 616 736 820 922 955 996
Size: 307 28 281 120 84 102 33 41
Cuts at: 996 1566
Size: 570
Fragments arranged by size:
570 307 281 120 102 84 41 33
28
NaeI GCC'GGC
Cuts at: 0 1525 1566
Size: 1525 41
NarI GG'CG_CC
Cuts at: 0 740 892 1566
Size: 740 152 674
Fragments arranged by size:
740 674 152
NcoI C'CATG_G
Cuts at: 0 320 1566
Size: 320 1246
NgoMIV G'CCGG_C
Cuts at: 0 1523 1566
Size: 1523 43
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 222 254 1566
Size: 222 32 1312
Fragments arranged by size:
1312 222 32
PspOMI G'GGCC_C
Cuts at: 0 29 652 1385 1566
Size: 29 623 733 181
Fragments arranged by size:
733 623 181 29
PstI C_TGCA'G
Cuts at: 0 960 1271 1415 1457 1487 1566
Size: 960 311 144 42 30 79
Fragments arranged by size:
960 311 144 79 42 30
PvuII CAG'CTG
Cuts at: 0 307 616 922 955 996 1566
Size: 307 309 306 33 41 570
Fragments arranged by size:
570 309 307 306 41 33
SacI G_AGCT'C
Cuts at: 0 1178 1566
Size: 1178 388
ScaI AGT'ACT
Cuts at: 0 648 1566
Size: 648 918
SfcI C'TryA_G
Cuts at: 0 35 86 956 1267 1411 1453 1483 1566
Size: 35 51 870 311 144 42 30 83
Fragments arranged by size:
870 311 144 83 51 42 35 30
SfoI GGC'GCC
Cuts at: 0 741 893 1566
Size: 741 152 673
Fragments arranged by size:
741 673 152
SmlI C'TyrA_G
Cuts at: 0 294 1330 1351 1566
Size: 294 1036 21 215
Fragments arranged by size:
1036 294 215 21
StyI C'CwwG_G
Cuts at: 0 320 1508 1566
Size: 320 1188 58
Fragments arranged by size:
1188 320 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 205 423 642 1081 1566
Size: 205 218 219 439 485
Fragments arranged by size:
485 439 219 218 205
TatI w'GTAC_w
Cuts at: 0 646 1566
Size: 646 920
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 180 212 1566
Size: 180 32 1354
Fragments arranged by size:
1354 180 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 318 441 1566
Size: 318 123 1125
Fragments arranged by size:
1125 318 123
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaI ApoI BamHI
BanI BanII BbeI BbsI BcgI BclI BglII BlpI
Bme1580I BmgBI BmrI BpmI BpuEI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BsrFI BstEII BstXI BstYI BtgI BtsI CspCI DraI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI MmeI
MslI MspA1I NaeI NarI NcoI NgoMIV PpiI PspOMI
PstI PvuII SacI ScaI SfcI SfoI SmlI StyI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbvCI BciVI BfrBI BglI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BspHI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtgZI ClaI DraIII DrdI EagI EcoRV FseI HindIII
HpaI KpnI MfeI MluI MscI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI