(Linear) (Six-base) MAPSORT of: KIBB8576.seq  Check: 3246  from: 1  to: 558



KIBB8576.orf

 With 182 enzymes: SgfI * 

                        February 20, 2009 16:36  ..

AccI GT'mk_AC

Cuts at:      0     521     558 
   Size:        521      37
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     558 
   Size:         58     500
 
AflIII A'CryG_T

Cuts at:      0     206     558 
   Size:        206     352
 
AhdI GACnn_n'nnGTC

Cuts at:      0      22     558 
   Size:         22     536
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     494     558 
   Size:        494      64
 
AlwNI CAG_nnn'CTG

Cuts at:      0     438     558 
   Size:        438     120
 
ApoI r'AATT_y

Cuts at:      0     169     365     558 
   Size:        169     196     193

  Fragments arranged by size: 

                 196     193     169
 
BstYI r'GATC_y

Cuts at:      0     396     539     558 
   Size:        396     143      19

  Fragments arranged by size: 

                 396     143      19
 
BtsI GCAGTG_nn'

Cuts at:      0     416     558 
   Size:        416     142
 
EaeI y'GGCC_r

Cuts at:      0     311     558 
   Size:        311     247
 
EarI CTCTTCn'nnn_

Cuts at:      0     338     558 
   Size:        338     220
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     558 
   Size:         58     500
 
EcoRI G'AATT_C

Cuts at:      0     365     558 
   Size:        365     193
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       8      40     558 
   Size:          8      32     518

  Fragments arranged by size: 

                 518      32       8
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     558 
   Size:        169     389
 
MscI TGG'CCA

Cuts at:      0     313     558 
   Size:        313     245
 
MslI CAynn'nnrTG

Cuts at:      0     476     558 
   Size:        476      82
 
NspI r_CATG'y

Cuts at:      0     210     558 
   Size:        210     348
 
PciI A'CATG_T

Cuts at:      0     206     558 
   Size:        206     352
 
PsiI TTA'TAA

Cuts at:      0     251     558 
   Size:        251     307
 
StyI C'CwwG_G

Cuts at:      0      66     558 
   Size:         66     492
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI     AlfI    AlwNI     ApoI    BstYI
     BtsI     EaeI     EarI  Eco57MI    EcoRI    Hin4I     MmeI     MscI
     MslI     NspI     PciI     PsiI     StyI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI