Restriction map for
pF1KB8545
(Linear) (Six-base) MAPSORT of: KIBB8545.seq Check: 2230 from: 1 to: 2952
KIBB8545.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 0 245 1845 2096 2952
Size: 245 1600 251 856
Fragments arranged by size:
1600 856 251 245
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 1584 2952
Size: 568 1016 1368
Fragments arranged by size:
1368 1016 568
AfeI AGC'GCT
Cuts at: 0 2766 2952
Size: 2766 186
AlwNI CAG_nnn'CTG
Cuts at: 0 534 1813 2952
Size: 534 1279 1139
Fragments arranged by size:
1279 1139 534
ApoI r'AATT_y
Cuts at: 0 1295 1339 1481 2120 2464 2845 2952
Size: 1295 44 142 639 344 381 107
Fragments arranged by size:
1295 639 381 344 142 107 44
AseI AT'TA_AT
Cuts at: 0 1535 2952
Size: 1535 1417
AvaI C'yCGr_G
Cuts at: 0 2084 2879 2952
Size: 2084 795 73
Fragments arranged by size:
2084 795 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 104 137 466 499 2952
Size: 104 33 329 33 2453
Fragments arranged by size:
2453 329 104 33 33
BanI G'GyrC_C
Cuts at: 0 1594 2693 2952
Size: 1594 1099 259
Fragments arranged by size:
1594 1099 259
BanII G_rGCy'C
Cuts at: 0 1521 2952
Size: 1521 1431
BbeI G_GCGC'C
Cuts at: 0 2697 2952
Size: 2697 255
BbsI GAAGACnn'nnnn_
Cuts at: 0 55 1461 2952
Size: 55 1406 1491
Fragments arranged by size:
1491 1406 55
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2698 2732 2952
Size: 2698 34 220
Fragments arranged by size:
2698 220 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2636 2885 2952
Size: 2636 249 67
Fragments arranged by size:
2636 249 67
BglII A'GATC_T
Cuts at: 0 1933 2952
Size: 1933 1019
BlpI GC'TnA_GC
Cuts at: 0 527 2952
Size: 527 2425
BmgBI CAC'GTC
Cuts at: 0 922 2952
Size: 922 2030
BmtI G_CTAG'C
Cuts at: 0 1676 2708 2952
Size: 1676 1032 244
Fragments arranged by size:
1676 1032 244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 523 1652 1709 2952
Size: 347 176 1129 57 1243
Fragments arranged by size:
1243 1129 347 176 57
Bpu10I CC'TnA_GC
Cuts at: 0 732 2952
Size: 732 2220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 1592 1729 1942 2952
Size: 755 837 137 213 1010
Fragments arranged by size:
1010 837 755 213 137
BsaI GGTCTCn'nnnn_
Cuts at: 0 1328 2952
Size: 1328 1624
BsaAI yAC'GTr
Cuts at: 0 1285 2952
Size: 1285 1667
BsaHI Gr'CG_yC
Cuts at: 0 4 2694 2952
Size: 4 2690 258
Fragments arranged by size:
2690 258 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1068 1098 2063 2093 2952
Size: 1068 30 965 30 859
Fragments arranged by size:
1068 965 859 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1511 1712 1874 2920 2952
Size: 1511 201 162 1046 32
Fragments arranged by size:
1511 1046 201 162 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 482 2952
Size: 142 340 2470
Fragments arranged by size:
2470 340 142
BsiHKAI G_wGCw'C
Cuts at: 0 1521 2952
Size: 1521 1431
BsmI GAATG_Cn'
Cuts at: 0 578 2952
Size: 578 2374
Bsp1286I G_dGCh'C
Cuts at: 0 1521 2952
Size: 1521 1431
BspHI T'CATG_A
Cuts at: 0 1103 2952
Size: 1103 1849
BsrDI GCAATG_nn'
Cuts at: 0 2936 2952
Size: 2936 16
BsrFI r'CCGG_y
Cuts at: 0 2221 2952
Size: 2221 731
BssSI C'ACGA_G
Cuts at: 0 1736 2952
Size: 1736 1216
BstAPI GCAn_nnn'nTGC
Cuts at: 0 703 2952
Size: 703 2249
BstEII G'GTnAC_C
Cuts at: 0 1425 2952
Size: 1425 1527
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1378 2952
Size: 1378 1574
BstYI r'GATC_y
Cuts at: 0 330 493 1933 2952
Size: 330 163 1440 1019
Fragments arranged by size:
1440 1019 330 163
BstZ17I GTA'TAC
Cuts at: 0 1846 2952
Size: 1846 1106
BtgI C'CryG_G
Cuts at: 0 516 2952
Size: 516 2436
BtsI GCAGTG_nn'
Cuts at: 0 1063 2952
Size: 1063 1889
DraI TTT'AAA
Cuts at: 0 812 2952
Size: 812 2140
EaeI y'GGCC_r
Cuts at: 0 1212 2952
Size: 1212 1740
EarI CTCTTCn'nnn_
Cuts at: 0 1560 1986 2579 2897 2952
Size: 1560 426 593 318 55
Fragments arranged by size:
1560 593 426 318 55
EcoICRI GAG'CTC
Cuts at: 0 1519 2952
Size: 1519 1433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 523 568 1584 1652 1709 2952
Size: 347 176 45 1016 68 57 1243
Fragments arranged by size:
1243 1016 347 176 68 57 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1889 2952
Size: 1889 1063
EcoO109I rG'GnC_Cy
Cuts at: 0 417 2229 2523 2952
Size: 417 1812 294 429
Fragments arranged by size:
1812 429 417 294
EcoRV GAT'ATC
Cuts at: 0 141 935 1020 2952
Size: 141 794 85 1932
Fragments arranged by size:
1932 794 141 85
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 254 1018 2952
Size: 254 764 1934
Fragments arranged by size:
1934 764 254
HaeII r_GCGC'y
Cuts at: 0 2697 2768 2952
Size: 2697 71 184
Fragments arranged by size:
2697 184 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 624 656 1150 1182 1306 1338 1918 1950
Size: 624 32 494 32 124 32 580 32
Cuts at: 1950 2862 2894 2952
Size: 912 32 58
Fragments arranged by size:
912 624 580 494 124 58 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 2693 2952
Size: 2693 259
MfeI C'AATT_G
Cuts at: 0 430 2952
Size: 430 2522
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 782 1054 2952
Size: 32 750 272 1898
Fragments arranged by size:
1898 750 272 32
MscI TGG'CCA
Cuts at: 0 1214 2952
Size: 1214 1738
MslI CAynn'nnrTG
Cuts at: 0 571 2252 2952
Size: 571 1681 700
Fragments arranged by size:
1681 700 571
NarI GG'CG_CC
Cuts at: 0 2694 2952
Size: 2694 258
NcoI C'CATG_G
Cuts at: 0 516 2952
Size: 516 2436
NheI G'CTAG_C
Cuts at: 0 1672 2704 2952
Size: 1672 1032 248
Fragments arranged by size:
1672 1032 248
NspI r_CATG'y
Cuts at: 0 1386 2177 2197 2952
Size: 1386 791 20 755
Fragments arranged by size:
1386 791 755 20
PfoI T'CCnGG_A
Cuts at: 0 539 2952
Size: 539 2413
PmlI CAC'GTG
Cuts at: 0 1285 2952
Size: 1285 1667
PpuMI rG'GwC_Cy
Cuts at: 0 2229 2952
Size: 2229 723
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2617 2649 2876 2908 2952
Size: 2617 32 227 32 44
Fragments arranged by size:
2617 227 44 32 32
PstI C_TGCA'G
Cuts at: 0 47 302 2952
Size: 47 255 2650
Fragments arranged by size:
2650 255 47
SacI G_AGCT'C
Cuts at: 0 1521 2952
Size: 1521 1431
SapI GCTCTTCn'nnn_
Cuts at: 0 1560 2952
Size: 1560 1392
SfcI C'TryA_G
Cuts at: 0 43 298 1577 1625 1682 2510 2642 2952
Size: 43 255 1279 48 57 828 132 310
Fragments arranged by size:
1279 828 310 255 132 57 48 43
SfoI GGC'GCC
Cuts at: 0 2695 2952
Size: 2695 257
SmlI C'TyrA_G
Cuts at: 0 770 1607 1708 1921 2084 2879 2952
Size: 770 837 101 213 163 795 73
Fragments arranged by size:
837 795 770 213 163 101 73
SspI AAT'ATT
Cuts at: 0 1533 1539 2952
Size: 1533 6 1413
Fragments arranged by size:
1533 1413 6
StuI AGG'CCT
Cuts at: 0 1895 2952
Size: 1895 1057
StyI C'CwwG_G
Cuts at: 0 355 516 2526 2629 2697 2887 2952
Size: 355 161 2010 103 68 190 65
Fragments arranged by size:
2010 355 190 161 103 68 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2753 2952
Size: 2753 199
TatI w'GTAC_w
Cuts at: 0 955 2952
Size: 955 1997
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1068 1100 2063 2095 2952
Size: 1068 32 963 32 857
Fragments arranged by size:
1068 963 857 32 32
XhoI C'TCGA_G
Cuts at: 0 2084 2879 2952
Size: 2084 795 73
Fragments arranged by size:
2084 795 73
Enzymes that do cut:
AccI AcuI AfeI AlwNI ApoI AseI AvaI BaeI
BanI BanII BbeI BbsI BcgI BciVI BglII BlpI
BmgBI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI BstZ17I BtgI
BtsI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI HaeII Hin4I KasI MfeI MmeI MscI
MslI NarI NcoI NheI NspI PfoI PmlI PpuMI
PsrI PstI SacI SapI SfcI SfoI SmlI SspI
StuI StyI TaqII TatI TstI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII
BamHI BbvCI BclI BfrBI BglI Bme1580I BmrI BplI
BsaBI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspMI
BsrBI BsrGI BssHII BstBI Bsu36I BtgZI ClaI CspCI
DraIII DrdI EagI EciI EcoRI FseI FspI FspAI
HincII HindIII HpaI KpnI MluI MspA1I NaeI NdeI
NgoMIV NotI NruI NsiI PacI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PspXI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XcmI XmaI XmnI ZraI