Restriction map for
pF1KB8538
(Linear) (Six-base) MAPSORT of: KIBB8538.seq Check: 2049 from: 1 to: 2694
KIBB8538.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 221 461 463 1319 1477 1781 2000
Size: 43 178 240 2 856 158 304 219
Cuts at: 2000 2694
Size: 694
Fragments arranged by size:
856 694 304 240 219 178 158 43
2
AflII C'TTAA_G
Cuts at: 0 976 2694
Size: 976 1718
AflIII A'CryG_T
Cuts at: 0 1448 1745 2576 2694
Size: 1448 297 831 118
Fragments arranged by size:
1448 831 297 118
ApaLI G'TGCA_C
Cuts at: 0 2415 2694
Size: 2415 279
ApoI r'AATT_y
Cuts at: 0 556 856 2182 2471 2694
Size: 556 300 1326 289 223
Fragments arranged by size:
1326 556 300 289 223
AseI AT'TA_AT
Cuts at: 0 1465 2694
Size: 1465 1229
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1586 1619 1831 1864 2023 2056 2694
Size: 1586 33 212 33 159 33 638
Fragments arranged by size:
1586 638 212 159 33 33 33
BanI G'GyrC_C
Cuts at: 0 472 2694
Size: 472 2222
BbsI GAAGACnn'nnnn_
Cuts at: 0 1001 1882 2694
Size: 1001 881 812
Fragments arranged by size:
1001 881 812
BciVI GTATCCnnnnn_n'
Cuts at: 0 161 2694
Size: 161 2533
BclI T'GATC_A
Cuts at: 0 362 2483 2694
Size: 362 2121 211
Fragments arranged by size:
2121 362 211
BfrBI ATG'CAT
Cuts at: 0 786 2694
Size: 786 1908
BglI GCCn_nnn'nGGC
Cuts at: 0 2212 2694
Size: 2212 482
BglII A'GATC_T
Cuts at: 0 1767 2694
Size: 1767 927
BlpI GC'TnA_GC
Cuts at: 0 1061 2694
Size: 1061 1633
Bme1580I G_kGCm'C
Cuts at: 0 477 2419 2694
Size: 477 1942 275
Fragments arranged by size:
1942 477 275
BmrI ACTGGGnnnn_n'
Cuts at: 0 84 1434 2492 2694
Size: 84 1350 1058 202
Fragments arranged by size:
1350 1058 202 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1913 2694
Size: 1913 781
Bpu10I CC'TnA_GC
Cuts at: 0 1334 2694
Size: 1334 1360
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 803 1804 2357 2694
Size: 626 177 1001 553 337
Fragments arranged by size:
1001 626 553 337 177
BsaI GGTCTCn'nnnn_
Cuts at: 0 602 2694
Size: 602 2092
BsaAI yAC'GTr
Cuts at: 0 1451 2694
Size: 1451 1243
BsaBI GATnn'nnATC
Cuts at: 0 1766 2694
Size: 1766 928
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 76 106 2694
Size: 19 30 27 30 2588
Fragments arranged by size:
2588 30 30 27 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 318 323 1564 2694
Size: 318 5 1241 1130
Fragments arranged by size:
1241 1130 318 5
BsiEI CG_ry'CG
Cuts at: 0 1048 2694
Size: 1048 1646
BsiHKAI G_wGCw'C
Cuts at: 0 738 2419 2694
Size: 738 1681 275
Fragments arranged by size:
1681 738 275
BsmI GAATG_Cn'
Cuts at: 0 2461 2694
Size: 2461 233
Bsp1286I G_dGCh'C
Cuts at: 0 477 738 2419 2694
Size: 477 261 1681 275
Fragments arranged by size:
1681 477 275 261
BspHI T'CATG_A
Cuts at: 0 545 1248 2694
Size: 545 703 1446
Fragments arranged by size:
1446 703 545
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1643 2694
Size: 1643 1051
BsrFI r'CCGG_y
Cuts at: 0 11 2694
Size: 11 2683
BsrGI T'GTAC_A
Cuts at: 0 1558 2694
Size: 1558 1136
BstAPI GCAn_nnn'nTGC
Cuts at: 0 69 472 2694
Size: 69 403 2222
Fragments arranged by size:
2222 403 69
BstBI TT'CG_AA
Cuts at: 0 759 2694
Size: 759 1935
BstEII G'GTnAC_C
Cuts at: 0 2098 2694
Size: 2098 596
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2238 2694
Size: 2238 456
BstYI r'GATC_y
Cuts at: 0 1767 2588 2694
Size: 1767 821 106
Fragments arranged by size:
1767 821 106
BtgI C'CryG_G
Cuts at: 0 2466 2694
Size: 2466 228
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2633 2694
Size: 2633 61
BtsI GCAGTG_nn'
Cuts at: 0 1533 1810 2694
Size: 1533 277 884
Fragments arranged by size:
1533 884 277
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1199 1234 2694
Size: 1199 35 1460
Fragments arranged by size:
1460 1199 35
DraI TTT'AAA
Cuts at: 0 311 1691 2604 2694
Size: 311 1380 913 90
Fragments arranged by size:
1380 913 311 90
DraIII CAC_nnn'GTG
Cuts at: 0 1428 2694
Size: 1428 1266
EaeI y'GGCC_r
Cuts at: 0 1030 2694
Size: 1030 1664
EarI CTCTTCn'nnn_
Cuts at: 0 1082 1541 1777 2660 2694
Size: 1082 459 236 883 34
Fragments arranged by size:
1082 883 459 236 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 221 461 463 1319 1477 1781 1913
Size: 43 178 240 2 856 158 304 132
Cuts at: 1913 2000 2694
Size: 87 694
Fragments arranged by size:
856 694 304 240 178 158 132 87
43 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 976 1546 2694
Size: 976 570 1148
Fragments arranged by size:
1148 976 570
EcoRI G'AATT_C
Cuts at: 0 2182 2694
Size: 2182 512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1255 2689 2694
Size: 1255 1434 5
Fragments arranged by size:
1434 1255 5
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 630 662 807 839 1044 1076
Size: 76 32 522 32 145 32 205 32
Cuts at: 1076 1777 1809 1938 1970 2694
Size: 701 32 129 32 724
Fragments arranged by size:
724 701 522 205 145 129 76 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 2111 2694
Size: 2111 583
MfeI C'AATT_G
Cuts at: 0 1292 2216 2345 2694
Size: 1292 924 129 349
Fragments arranged by size:
1292 924 349 129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 2694
Size: 413 2281
MslI CAynn'nnrTG
Cuts at: 0 2236 2694
Size: 2236 458
MspA1I CmG'CkG
Cuts at: 0 75 2007 2694
Size: 75 1932 687
Fragments arranged by size:
1932 687 75
NsiI A_TGCA'T
Cuts at: 0 788 2694
Size: 788 1906
NspI r_CATG'y
Cuts at: 0 614 786 807 1311 1400 1749 2052 2128
Size: 614 172 21 504 89 349 303 76
Cuts at: 2128 2580 2694
Size: 452 114
Fragments arranged by size:
614 504 452 349 303 172 114 89
76 21
PciI A'CATG_T
Cuts at: 0 1745 2576 2694
Size: 1745 831 118
Fragments arranged by size:
1745 831 118
PfoI T'CCnGG_A
Cuts at: 0 1490 2694
Size: 1490 1204
PsiI TTA'TAA
Cuts at: 0 449 2404 2694
Size: 449 1955 290
Fragments arranged by size:
1955 449 290
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 698 730 1010 1042 2694
Size: 698 32 280 32 1652
Fragments arranged by size:
1652 698 280 32 32
PstI C_TGCA'G
Cuts at: 0 74 1528 1817 2694
Size: 74 1454 289 877
Fragments arranged by size:
1454 877 289 74
PvuI CG_AT'CG
Cuts at: 0 1048 2694
Size: 1048 1646
PvuII CAG'CTG
Cuts at: 0 75 2007 2694
Size: 75 1932 687
Fragments arranged by size:
1932 687 75
ScaI AGT'ACT
Cuts at: 0 952 2694
Size: 952 1742
SfcI C'TryA_G
Cuts at: 0 70 371 423 808 1524 1813 2392 2694
Size: 70 301 52 385 716 289 579 302
Fragments arranged by size:
716 579 385 302 301 289 70 52
SmlI C'TyrA_G
Cuts at: 0 641 818 976 1783 2372 2694
Size: 641 177 158 807 589 322
Fragments arranged by size:
807 641 589 322 177 158
SphI G_CATG'C
Cuts at: 0 1311 2052 2694
Size: 1311 741 642
Fragments arranged by size:
1311 741 642
SspI AAT'ATT
Cuts at: 0 318 2159 2694
Size: 318 1841 535
Fragments arranged by size:
1841 535 318
StyI C'CwwG_G
Cuts at: 0 380 2694
Size: 380 2314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1416 2694
Size: 1416 1278
TatI w'GTAC_w
Cuts at: 0 800 897 950 1509 1558 1869 2694
Size: 800 97 53 559 49 311 825
Fragments arranged by size:
825 800 559 311 97 53 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 19 51 2694
Size: 19 32 2643
Fragments arranged by size:
2643 32 19
XmnI GAAnn'nnTTC
Cuts at: 0 1004 1155 1568 1706 2694
Size: 1004 151 413 138 988
Fragments arranged by size:
1004 988 413 151 138
Enzymes that do cut:
AcuI AflII AflIII ApaLI ApoI AseI BaeI BanI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI
BseRI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI
BsrGI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII EaeI EarI Eco57MI EcoNI
EcoRI FalI Hin4I HindIII MfeI MmeI MslI MspA1I
NsiI NspI PciI PfoI PsiI PsrI PstI PvuI
PvuII ScaI SfcI SmlI SphI SspI StyI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI AlwNI ApaI AscI AsiSI AvaI
AvrII BamHI BanII BbeI BbvCI BcgI BmgBI BmtI
BplI BsaHI BsaWI BseYI BsgI BsiWI BsmBI BspEI
BsrBI BsrDI BssHII BssSI BstZ17I Bsu36I ClaI DrdI
EagI EciI EcoICRI EcoO109I EcoRV FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
ZraI