Restriction map for
pF1KB8505
(Linear) (Six-base) MAPSORT of: KIBB8505.seq Check: 897 from: 1 to: 2751
KIBB8505.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AclI AA'CG_TT
Cuts at: 0 1020 1400 2751
Size: 1020 380 1351
Fragments arranged by size:
1351 1020 380
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 456 1739 2751
Size: 63 393 1283 1012
Fragments arranged by size:
1283 1012 393 63
AflII C'TTAA_G
Cuts at: 0 1806 2751
Size: 1806 945
AflIII A'CryG_T
Cuts at: 0 1975 2751
Size: 1975 776
AhdI GACnn_n'nnGTC
Cuts at: 0 587 2751
Size: 587 2164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2398 2751
Size: 2398 353
AlwNI CAG_nnn'CTG
Cuts at: 0 1378 2751
Size: 1378 1373
ApaI G_GGCC'C
Cuts at: 0 2713 2751
Size: 2713 38
ApaLI G'TGCA_C
Cuts at: 0 2060 2751
Size: 2060 691
ApoI r'AATT_y
Cuts at: 0 339 1335 2751
Size: 339 996 1416
Fragments arranged by size:
1416 996 339
AseI AT'TA_AT
Cuts at: 0 406 2751
Size: 406 2345
AvrII C'CTAG_G
Cuts at: 0 1393 2751
Size: 1393 1358
BanII G_rGCy'C
Cuts at: 0 421 2435 2713 2751
Size: 421 2014 278 38
Fragments arranged by size:
2014 421 278 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 583 1152 1646 2474 2751
Size: 583 569 494 828 277
Fragments arranged by size:
828 583 569 494 277
BclI T'GATC_A
Cuts at: 0 1947 2751
Size: 1947 804
BfrBI ATG'CAT
Cuts at: 0 2581 2751
Size: 2581 170
BglI GCCn_nnn'nGGC
Cuts at: 0 1363 2751
Size: 1363 1388
BlpI GC'TnA_GC
Cuts at: 0 664 2751
Size: 664 2087
Bme1580I G_kGCm'C
Cuts at: 0 171 2064 2713 2751
Size: 171 1893 649 38
Fragments arranged by size:
1893 649 171 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 174 716 1563 1926 2707 2751
Size: 174 542 847 363 781 44
Fragments arranged by size:
847 781 542 363 174 44
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1464 2751
Size: 1464 1287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 2751
Size: 406 2345
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 2086 2293 2751
Size: 494 1592 207 458
Fragments arranged by size:
1592 494 458 207
BsaBI GATnn'nnATC
Cuts at: 0 1517 2751
Size: 1517 1234
BsaHI Gr'CG_yC
Cuts at: 0 2419 2751
Size: 2419 332
BsaWI w'CCGG_w
Cuts at: 0 2548 2751
Size: 2548 203
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2617 2647 2751
Size: 2617 30 104
Fragments arranged by size:
2617 104 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 197 2083 2751
Size: 197 1886 668
Fragments arranged by size:
1886 668 197
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2270 2751
Size: 2270 481
BsiHKAI G_wGCw'C
Cuts at: 0 421 2064 2435 2751
Size: 421 1643 371 316
Fragments arranged by size:
1643 421 371 316
Bsp1286I G_dGCh'C
Cuts at: 0 171 421 2064 2435 2713 2751
Size: 171 250 1643 371 278 38
Fragments arranged by size:
1643 371 278 250 171 38
BspHI T'CATG_A
Cuts at: 0 972 1014 2721 2751
Size: 972 42 1707 30
Fragments arranged by size:
1707 972 42 30
BsrDI GCAATG_nn'
Cuts at: 0 553 950 1879 2091 2279 2751
Size: 553 397 929 212 188 472
Fragments arranged by size:
929 553 472 397 212 188
BstBI TT'CG_AA
Cuts at: 0 1845 2751
Size: 1845 906
BstYI r'GATC_y
Cuts at: 0 1287 1884 2072 2751
Size: 1287 597 188 679
Fragments arranged by size:
1287 679 597 188
Bsu36I CC'TnA_GG
Cuts at: 0 425 2751
Size: 425 2326
BtgI C'CryG_G
Cuts at: 0 440 2751
Size: 440 2311
BtsI GCAGTG_nn'
Cuts at: 0 1373 2751
Size: 1373 1378
DraI TTT'AAA
Cuts at: 0 9 2751
Size: 9 2742
EaeI y'GGCC_r
Cuts at: 0 377 2751
Size: 377 2374
EarI CTCTTCn'nnn_
Cuts at: 0 82 197 1496 2751
Size: 82 115 1299 1255
Fragments arranged by size:
1299 1255 115 82
EcoICRI GAG'CTC
Cuts at: 0 419 2433 2751
Size: 419 2014 318
Fragments arranged by size:
2014 419 318
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 406 456 1739 2751
Size: 63 343 50 1283 1012
Fragments arranged by size:
1283 1012 343 63 50
EcoO109I rG'GnC_Cy
Cuts at: 0 2117 2709 2751
Size: 2117 592 42
Fragments arranged by size:
2117 592 42
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 910 1114 2751
Size: 910 204 1637
Fragments arranged by size:
1637 910 204
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 573 605 685 717 1489 1521 2404 2436
Size: 573 32 80 32 772 32 883 32
Cuts at: 2436 2751
Size: 315
Fragments arranged by size:
883 772 573 315 80 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2397 2751
Size: 2397 354
HindIII A'AGCT_T
Cuts at: 0 1610 2021 2202 2751
Size: 1610 411 181 549
Fragments arranged by size:
1610 549 411 181
HpaI GTT'AAC
Cuts at: 0 2397 2751
Size: 2397 354
MfeI C'AATT_G
Cuts at: 0 65 2258 2354 2751
Size: 65 2193 96 397
Fragments arranged by size:
2193 397 96 65
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 2751
Size: 308 2443
MscI TGG'CCA
Cuts at: 0 379 2751
Size: 379 2372
MslI CAynn'nnrTG
Cuts at: 0 534 2726 2751
Size: 534 2192 25
Fragments arranged by size:
2192 534 25
MspA1I CmG'CkG
Cuts at: 0 1663 2751
Size: 1663 1088
NcoI C'CATG_G
Cuts at: 0 440 2751
Size: 440 2311
NsiI A_TGCA'T
Cuts at: 0 2583 2751
Size: 2583 168
NspI r_CATG'y
Cuts at: 0 930 1074 1979 2084 2751
Size: 930 144 905 105 667
Fragments arranged by size:
930 905 667 144 105
PciI A'CATG_T
Cuts at: 0 1975 2751
Size: 1975 776
PfoI T'CCnGG_A
Cuts at: 0 2207 2539 2751
Size: 2207 332 212
Fragments arranged by size:
2207 332 212
PpuMI rG'GwC_Cy
Cuts at: 0 2117 2751
Size: 2117 634
PshAI GACnn'nnGTC
Cuts at: 0 941 2751
Size: 941 1810
PsiI TTA'TAA
Cuts at: 0 1922 2751
Size: 1922 829
PspOMI G'GGCC_C
Cuts at: 0 2709 2751
Size: 2709 42
PstI C_TGCA'G
Cuts at: 0 1310 2751
Size: 1310 1441
PvuII CAG'CTG
Cuts at: 0 1663 2751
Size: 1663 1088
SacI G_AGCT'C
Cuts at: 0 421 2435 2751
Size: 421 2014 316
Fragments arranged by size:
2014 421 316
SapI GCTCTTCn'nnn_
Cuts at: 0 197 2751
Size: 197 2554
ScaI AGT'ACT
Cuts at: 0 2555 2751
Size: 2555 196
SfcI C'TryA_G
Cuts at: 0 1306 2751
Size: 1306 1445
SmlI C'TyrA_G
Cuts at: 0 509 1806 2065 2308 2751
Size: 509 1297 259 243 443
Fragments arranged by size:
1297 509 443 259 243
SspI AAT'ATT
Cuts at: 0 778 1041 1621 2751
Size: 778 263 580 1130
Fragments arranged by size:
1130 778 580 263
StuI AGG'CCT
Cuts at: 0 2314 2751
Size: 2314 437
StyI C'CwwG_G
Cuts at: 0 440 1103 1393 2751
Size: 440 663 290 1358
Fragments arranged by size:
1358 663 440 290
TatI w'GTAC_w
Cuts at: 0 540 2553 2751
Size: 540 2013 198
Fragments arranged by size:
2013 540 198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2203 2235 2617 2649 2751
Size: 2203 32 382 32 102
Fragments arranged by size:
2203 382 102 32 32
XbaI T'CTAG_A
Cuts at: 0 1578 2004 2751
Size: 1578 426 747
Fragments arranged by size:
1578 747 426
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 753 1365 2751
Size: 753 612 1386
Fragments arranged by size:
1386 753 612
XmnI GAAnn'nnTTC
Cuts at: 0 362 1094 1402 1652 2022 2751
Size: 362 732 308 250 370 729
Fragments arranged by size:
732 729 370 362 308 250
Enzymes that do cut:
AclI AcuI AflII AflIII AhdI AlfI AlwNI ApaI
ApaLI ApoI AseI AvrII BanII BbsI BclI BfrBI
BglI BlpI Bme1580I BmrI BplI BpmI BpuEI BsaBI
BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspHI
BsrDI BstBI BstYI Bsu36I BtgI BtsI DraI EaeI
EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NcoI NsiI
NspI PciI PfoI PpuMI PshAI PsiI PspOMI PstI
PvuII SacI SapI ScaI SfcI SmlI SspI StuI
StyI TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AgeI AleI AloI
AscI AsiSI AvaI BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BglII BmgBI BmtI Bpu10I BsaI BsaAI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII KasI KpnI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PflMI PmeI PmlI PpiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI TaqII Tth111I XhoI XmaI ZraI