Restriction map for
pF1KB8478
(Linear) (Six-base) MAPSORT of: KIBB8478.seq Check: 4030 from: 1 to: 2541
KIBB8478.orf
With 182 enzymes: SgfI *
March 2, 2011 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1045 1337 1377 2457 2541
Size: 1045 292 40 1080 84
Fragments arranged by size:
1080 1045 292 84 40
AatII G_ACGT'C
Cuts at: 0 914 1653 2541
Size: 914 739 888
Fragments arranged by size:
914 888 739
Acc65I G'GTAC_C
Cuts at: 0 1769 2541
Size: 1769 772
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 2541
Size: 808 1733
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1525 1966 2541
Size: 1525 441 575
Fragments arranged by size:
1525 575 441
AlwNI CAG_nnn'CTG
Cuts at: 0 348 2541
Size: 348 2193
ApaI G_GGCC'C
Cuts at: 0 599 2473 2541
Size: 599 1874 68
Fragments arranged by size:
1874 599 68
AvaI C'yCGr_G
Cuts at: 0 215 302 812 1042 1858 2541
Size: 215 87 510 230 816 683
Fragments arranged by size:
816 683 510 230 215 87
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2133 2166 2541
Size: 2133 33 375
Fragments arranged by size:
2133 375 33
BanI G'GyrC_C
Cuts at: 0 702 850 957 1769 1942 2251 2260 2506
Size: 702 148 107 812 173 309 9 246
Cuts at: 2506 2523 2541
Size: 17 18
Fragments arranged by size:
812 702 309 246 173 148 107 18
17 9
BanII G_rGCy'C
Cuts at: 0 8 34 161 560 599 612 1049 1859
Size: 8 26 127 399 39 13 437 810
Cuts at: 1859 1868 2182 2201 2473 2541
Size: 9 314 19 272 68
Fragments arranged by size:
810 437 399 314 272 127 68 39
26 19 13 9 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1539 2541
Size: 1539 1002
BbvCI CC'TCA_GC
Cuts at: 0 601 2541
Size: 601 1940
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1467 1501 2541
Size: 1467 34 1040
Fragments arranged by size:
1467 1040 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2161 2541
Size: 2161 380
BglI GCCn_nnn'nGGC
Cuts at: 0 165 275 511 538 865 1872 2541
Size: 165 110 236 27 327 1007 669
Fragments arranged by size:
1007 669 327 236 165 110 27
Bme1580I G_kGCm'C
Cuts at: 0 599 707 853 2473 2490 2511 2541
Size: 599 108 146 1620 17 21 30
Fragments arranged by size:
1620 599 146 108 30 21 17
BmgBI CAC'GTC
Cuts at: 0 2188 2541
Size: 2188 353
BmrI ACTGGGnnnn_n'
Cuts at: 0 155 660 1332 1697 2541
Size: 155 505 672 365 844
Fragments arranged by size:
844 672 505 365 155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 571 936 1156 1675 1873 2355 2541
Size: 376 195 365 220 519 198 482 186
Fragments arranged by size:
519 482 376 365 220 198 195 186
Bpu10I CC'TnA_GC
Cuts at: 0 601 2541
Size: 601 1940
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 520 709 1647 2083 2541
Size: 10 510 189 938 436 458
Fragments arranged by size:
938 510 458 436 189 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 107 1219 2541
Size: 107 1112 1322
Fragments arranged by size:
1322 1112 107
BsaAI yAC'GTr
Cuts at: 0 1410 2541
Size: 1410 1131
BsaHI Gr'CG_yC
Cuts at: 0 911 1650 1909 2104 2541
Size: 911 739 259 195 437
Fragments arranged by size:
911 739 437 259 195
BsaWI w'CCGG_w
Cuts at: 0 1532 2541
Size: 1532 1009
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 196 226 1093 1123 2198 2228 2541
Size: 196 30 867 30 1075 30 313
Fragments arranged by size:
1075 867 313 196 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 98 373 568 1276 1279 1551 1752 1876
Size: 98 275 195 708 3 272 201 124
Cuts at: 1876 2206 2209 2541
Size: 330 3 332
Fragments arranged by size:
708 332 330 275 272 201 195 124
98 3 3
BseYI C'CCAG_C
Cuts at: 0 1073 1688 1818 1962 2350 2541
Size: 1073 615 130 144 388 191
Fragments arranged by size:
1073 615 388 191 144 130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 679 1531 2111 2372 2541
Size: 670 9 852 580 261 169
Fragments arranged by size:
852 670 580 261 169 9
BsiEI CG_ry'CG
Cuts at: 0 1405 2063 2541
Size: 1405 658 478
Fragments arranged by size:
1405 658 478
BsiHKAI G_wGCw'C
Cuts at: 0 612 779 1328 2403 2541
Size: 612 167 549 1075 138
Fragments arranged by size:
1075 612 549 167 138
BsmBI CGTCTCn'nnnn_
Cuts at: 0 230 2541
Size: 230 2311
Bsp1286I G_dGCh'C
Cuts at: 0 8 34 161 560 599 612 707 779
Size: 8 26 127 399 39 13 95 72
Cuts at: 779 853 1049 1328 1859 1868 2182 2201 2403
Size: 74 196 279 531 9 314 19 202
Cuts at: 2403 2473 2490 2511 2541
Size: 70 17 21 30
Fragments arranged by size:
531 399 314 279 202 196 127 95
74 72 70 39 30 26 21 19
17 13 9 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 692 1045 1337 1377 1988 2457 2541
Size: 692 353 292 40 611 469 84
Fragments arranged by size:
692 611 469 353 292 84 40
BsrBI CCG'CTC
Cuts at: 0 1279 1677 2082 2541
Size: 1279 398 405 459
Fragments arranged by size:
1279 459 405 398
BsrDI GCAATG_nn'
Cuts at: 0 527 1674 2541
Size: 527 1147 867
Fragments arranged by size:
1147 867 527
BsrFI r'CCGG_y
Cuts at: 0 89 589 2541
Size: 89 500 1952
Fragments arranged by size:
1952 500 89
BsrGI T'GTAC_A
Cuts at: 0 392 2541
Size: 392 2149
BssHII G'CGCG_C
Cuts at: 0 1282 1453 2541
Size: 1282 171 1088
Fragments arranged by size:
1282 1088 171
BstAPI GCAn_nnn'nTGC
Cuts at: 0 684 977 2541
Size: 684 293 1564
Fragments arranged by size:
1564 684 293
BstEII G'GTnAC_C
Cuts at: 0 193 1831 2541
Size: 193 1638 710
Fragments arranged by size:
1638 710 193
BstXI CCAn_nnnn'nTGG
Cuts at: 0 265 1602 2502 2541
Size: 265 1337 900 39
Fragments arranged by size:
1337 900 265 39
BstYI r'GATC_y
Cuts at: 0 1205 1801 2541
Size: 1205 596 740
Fragments arranged by size:
1205 740 596
Bsu36I CC'TnA_GG
Cuts at: 0 95 184 872 1797 2541
Size: 95 89 688 925 744
Fragments arranged by size:
925 744 688 95 89
BtgI C'CryG_G
Cuts at: 0 669 845 966 1162 1422 1840 2202 2356
Size: 669 176 121 196 260 418 362 154
Cuts at: 2356 2518 2541
Size: 162 23
Fragments arranged by size:
669 418 362 260 196 176 162 154
121 23
BtsI GCAGTG_nn'
Cuts at: 0 60 687 912 1018 2541
Size: 60 627 225 106 1523
Fragments arranged by size:
1523 627 225 106 60
EaeI y'GGCC_r
Cuts at: 0 267 332 614 1064 1971 2541
Size: 267 65 282 450 907 570
Fragments arranged by size:
907 570 450 282 267 65
EarI CTCTTCn'nnn_
Cuts at: 0 7 25 505 1222 2541
Size: 7 18 480 717 1319
Fragments arranged by size:
1319 717 480 18 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1318 2541
Size: 1318 1223
EcoICRI GAG'CTC
Cuts at: 0 610 2541
Size: 610 1931
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 571 808 936 1156 1675 1873 2355
Size: 376 195 237 128 220 519 198 482
Cuts at: 2355 2541
Size: 186
Fragments arranged by size:
519 482 376 237 220 198 195 186
128
EcoNI CCTnn'n_nnAGG
Cuts at: 0 187 2541
Size: 187 2354
EcoO109I rG'GnC_Cy
Cuts at: 0 444 596 696 1083 1146 1699 1745 2469
Size: 444 152 100 387 63 553 46 724
Cuts at: 2469 2470 2514 2536 2541
Size: 1 44 22 5
Fragments arranged by size:
724 553 444 387 152 100 63 46
44 22 5 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 36 2541
Size: 36 2505
FseI GG_CCGG'CC
Cuts at: 0 93 2541
Size: 93 2448
HaeII r_GCGC'y
Cuts at: 0 1135 1933 2541
Size: 1135 798 608
Fragments arranged by size:
1135 798 608
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 196 228 712 744 1093 1125 1197 1229
Size: 196 32 484 32 349 32 72 32
Cuts at: 1229 1279 1311 1774 1806 2198 2230 2541
Size: 50 32 463 32 392 32 311
Fragments arranged by size:
484 463 392 349 311 196 72 50
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 648 1008 2541
Size: 648 360 1533
Fragments arranged by size:
1533 648 360
HpaI GTT'AAC
Cuts at: 0 1008 2541
Size: 1008 1533
KpnI G_GTAC'C
Cuts at: 0 1773 2541
Size: 1773 768
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 755 1106 1583 2541
Size: 314 441 351 477 958
Fragments arranged by size:
958 477 441 351 314
MscI TGG'CCA
Cuts at: 0 269 334 2541
Size: 269 65 2207
Fragments arranged by size:
2207 269 65
MslI CAynn'nnrTG
Cuts at: 0 463 835 1507 2541
Size: 463 372 672 1034
Fragments arranged by size:
1034 672 463 372
MspA1I CmG'CkG
Cuts at: 0 62 115 404 847 1164 1293 1311 1424
Size: 62 53 289 443 317 129 18 113
Cuts at: 1424 1842 1926 2166 2204 2350 2541
Size: 418 84 240 38 146 191
Fragments arranged by size:
443 418 317 289 240 191 146 129
113 84 62 53 38 18
NaeI GCC'GGC
Cuts at: 0 91 2541
Size: 91 2450
NgoMIV G'CCGG_C
Cuts at: 0 89 2541
Size: 89 2452
NspI r_CATG'y
Cuts at: 0 1482 1688 2541
Size: 1482 206 853
Fragments arranged by size:
1482 853 206
PasI CC'CwG_GG
Cuts at: 0 2280 2541
Size: 2280 261
PflMI CCAn_nnn'nTGG
Cuts at: 0 642 967 2541
Size: 642 325 1574
Fragments arranged by size:
1574 642 325
PfoI T'CCnGG_A
Cuts at: 0 341 2417 2541
Size: 341 2076 124
Fragments arranged by size:
2076 341 124
PmlI CAC'GTG
Cuts at: 0 1410 2541
Size: 1410 1131
PpuMI rG'GwC_Cy
Cuts at: 0 444 1083 1146 1699 1745 2541
Size: 444 639 63 553 46 796
Fragments arranged by size:
796 639 553 444 63 46
PspOMI G'GGCC_C
Cuts at: 0 595 2469 2541
Size: 595 1874 72
Fragments arranged by size:
1874 595 72
PstI C_TGCA'G
Cuts at: 0 752 1121 1385 1614 1985 2485 2541
Size: 752 369 264 229 371 500 56
Fragments arranged by size:
752 500 371 369 264 229 56
PvuII CAG'CTG
Cuts at: 0 404 1311 2350 2541
Size: 404 907 1039 191
Fragments arranged by size:
1039 907 404 191
SacI G_AGCT'C
Cuts at: 0 612 2541
Size: 612 1929
SacII CC_GC'GG
Cuts at: 0 848 1165 1425 1843 2205 2541
Size: 848 317 260 418 362 336
Fragments arranged by size:
848 418 362 336 317 260
SanDI GG'GwC_CC
Cuts at: 0 1699 2541
Size: 1699 842
SapI GCTCTTCn'nnn_
Cuts at: 0 7 505 2541
Size: 7 498 2036
Fragments arranged by size:
2036 498 7
SfcI C'TryA_G
Cuts at: 0 748 1117 1381 1610 1981 2481 2541
Size: 748 369 264 229 371 500 60
Fragments arranged by size:
748 500 371 369 264 229 60
SmaI CCC'GGG
Cuts at: 0 217 2541
Size: 217 2324
SmlI C'TyrA_G
Cuts at: 0 25 535 724 1662 2098 2541
Size: 25 510 189 938 436 443
Fragments arranged by size:
938 510 443 436 189 25
SphI G_CATG'C
Cuts at: 0 1482 1688 2541
Size: 1482 206 853
Fragments arranged by size:
1482 853 206
StuI AGG'CCT
Cuts at: 0 886 1516 1875 2541
Size: 886 630 359 666
Fragments arranged by size:
886 666 630 359
StyI C'CwwG_G
Cuts at: 0 1235 1968 2541
Size: 1235 733 573
Fragments arranged by size:
1235 733 573
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 253 2077 2494 2511 2541
Size: 253 1824 417 17 30
Fragments arranged by size:
1824 417 253 30 17
TatI w'GTAC_w
Cuts at: 0 392 2541
Size: 392 2149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 39 71 1010 1042 2541
Size: 39 32 939 32 1499
Fragments arranged by size:
1499 939 39 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 567 1625 1770 2541
Size: 567 1058 145 771
Fragments arranged by size:
1058 771 567 145
XmaI C'CCGG_G
Cuts at: 0 215 2541
Size: 215 2326
ZraI GAC'GTC
Cuts at: 0 912 1651 2541
Size: 912 739 890
Fragments arranged by size:
912 890 739
Enzymes that do cut:
AarI AatII Acc65I AcuI AlfI AlwNI ApaI AvaI
BaeI BanI BanII BbsI BbvCI BcgI BciVI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI
HaeII Hin4I HincII HpaI KpnI MmeI MscI MslI
MspA1I NaeI NgoMIV NspI PasI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI
SfcI SmaI SmlI SphI StuI StyI TaqII TatI
TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AgeI AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BamHI
BbeI BclI BfrBI BglII BlpI BmtI BplI BsaBI
BsiWI BsmI BspEI BspHI BssSI BstBI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV
FspI FspAI HindIII KasI MfeI MluI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmnI