Restriction map for
pF1KB8447
(Linear) (Six-base) MAPSORT of: KIBB8447.seq Check: 1007 from: 1 to: 1407
KIBB8447.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
Acc65I G'GTAC_C
Cuts at: 0 969 1407
Size: 969 438
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 889 1407
Size: 119 770 518
Fragments arranged by size:
770 518 119
AflIII A'CryG_T
Cuts at: 0 144 313 1407
Size: 144 169 1094
Fragments arranged by size:
1094 169 144
AlwNI CAG_nnn'CTG
Cuts at: 0 852 1407
Size: 852 555
ApaI G_GGCC'C
Cuts at: 0 1231 1407
Size: 1231 176
ApaLI G'TGCA_C
Cuts at: 0 10 1407
Size: 10 1397
ApoI r'AATT_y
Cuts at: 0 79 113 281 558 801 1407
Size: 79 34 168 277 243 606
Fragments arranged by size:
606 277 243 168 79 34
AvrII C'CTAG_G
Cuts at: 0 1083 1407
Size: 1083 324
BanI G'GyrC_C
Cuts at: 0 969 1407
Size: 969 438
BanII G_rGCy'C
Cuts at: 0 607 1231 1407
Size: 607 624 176
Fragments arranged by size:
624 607 176
BbsI GAAGACnn'nnnn_
Cuts at: 0 667 1407
Size: 667 740
BglI GCCn_nnn'nGGC
Cuts at: 0 1154 1407
Size: 1154 253
Bme1580I G_kGCm'C
Cuts at: 0 14 1231 1407
Size: 14 1217 176
Fragments arranged by size:
1217 176 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 712 1110 1407
Size: 712 398 297
Fragments arranged by size:
712 398 297
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 699 1407
Size: 424 275 708
Fragments arranged by size:
708 424 275
BsaHI Gr'CG_yC
Cuts at: 0 724 1376 1407
Size: 724 652 31
Fragments arranged by size:
724 652 31
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 390 1407
Size: 390 1017
BseYI C'CCAG_C
Cuts at: 0 1390 1407
Size: 1390 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1099 1407
Size: 485 614 308
Fragments arranged by size:
614 485 308
BsiHKAI G_wGCw'C
Cuts at: 0 14 607 1407
Size: 14 593 800
Fragments arranged by size:
800 593 14
BsiWI C'GTAC_G
Cuts at: 0 1274 1407
Size: 1274 133
BsmBI CGTCTCn'nnnn_
Cuts at: 0 610 1407
Size: 610 797
Bsp1286I G_dGCh'C
Cuts at: 0 14 607 1231 1407
Size: 14 593 624 176
Fragments arranged by size:
624 593 176 14
BspHI T'CATG_A
Cuts at: 0 249 1407
Size: 249 1158
BsrDI GCAATG_nn'
Cuts at: 0 995 1407
Size: 995 412
BsrFI r'CCGG_y
Cuts at: 0 1105 1407
Size: 1105 302
BstBI TT'CG_AA
Cuts at: 0 551 738 1407
Size: 551 187 669
Fragments arranged by size:
669 551 187
BstEII G'GTnAC_C
Cuts at: 0 474 1407
Size: 474 933
Bsu36I CC'TnA_GG
Cuts at: 0 1294 1407
Size: 1294 113
BtgI C'CryG_G
Cuts at: 0 401 535 782 1035 1340 1407
Size: 401 134 247 253 305 67
Fragments arranged by size:
401 305 253 247 134 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1293 1407
Size: 1293 114
BtsI GCAGTG_nn'
Cuts at: 0 607 1407
Size: 607 800
ClaI AT'CG_AT
Cuts at: 0 791 1407
Size: 791 616
EaeI y'GGCC_r
Cuts at: 0 1141 1407
Size: 1141 266
EarI CTCTTCn'nnn_
Cuts at: 0 38 1407
Size: 38 1369
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 974 1407
Size: 974 433
EcoICRI GAG'CTC
Cuts at: 0 605 1407
Size: 605 802
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 889 1407
Size: 119 770 518
Fragments arranged by size:
770 518 119
EcoNI CCTnn'n_nnAGG
Cuts at: 0 229 1407
Size: 229 1178
EcoO109I rG'GnC_Cy
Cuts at: 0 1021 1227 1228 1407
Size: 1021 206 1 179
Fragments arranged by size:
1021 206 179 1
EcoRI G'AATT_C
Cuts at: 0 801 1407
Size: 801 606
EcoRV GAT'ATC
Cuts at: 0 183 1407
Size: 183 1224
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 495 1407
Size: 495 912
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 973 1005 1407
Size: 973 32 402
Fragments arranged by size:
973 402 32
HincII GTy'rAC
Cuts at: 0 843 1407
Size: 843 564
HindIII A'AGCT_T
Cuts at: 0 442 1407
Size: 442 965
HpaI GTT'AAC
Cuts at: 0 843 1407
Size: 843 564
KpnI G_GTAC'C
Cuts at: 0 973 1407
Size: 973 434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 1298 1407
Size: 387 911 109
Fragments arranged by size:
911 387 109
MslI CAynn'nnrTG
Cuts at: 0 155 1407
Size: 155 1252
MspA1I CmG'CkG
Cuts at: 0 637 1037 1268 1407
Size: 637 400 231 139
Fragments arranged by size:
637 400 231 139
NaeI GCC'GGC
Cuts at: 0 1107 1407
Size: 1107 300
NcoI C'CATG_G
Cuts at: 0 401 782 1340 1407
Size: 401 381 558 67
Fragments arranged by size:
558 401 381 67
NdeI CA'TA_TG
Cuts at: 0 655 1407
Size: 655 752
NgoMIV G'CCGG_C
Cuts at: 0 1105 1407
Size: 1105 302
NspI r_CATG'y
Cuts at: 0 148 317 1050 1407
Size: 148 169 733 357
Fragments arranged by size:
733 357 169 148
PciI A'CATG_T
Cuts at: 0 144 313 1407
Size: 144 169 1094
Fragments arranged by size:
1094 169 144
PflMI CCAn_nnn'nTGG
Cuts at: 0 43 852 1407
Size: 43 809 555
Fragments arranged by size:
809 555 43
PpuMI rG'GwC_Cy
Cuts at: 0 1021 1407
Size: 1021 386
PshAI GACnn'nnGTC
Cuts at: 0 314 1407
Size: 314 1093
PsiI TTA'TAA
Cuts at: 0 395 1407
Size: 395 1012
PspOMI G'GGCC_C
Cuts at: 0 1227 1407
Size: 1227 180
PvuII CAG'CTG
Cuts at: 0 637 1407
Size: 637 770
SacI G_AGCT'C
Cuts at: 0 607 1407
Size: 607 800
SacII CC_GC'GG
Cuts at: 0 1038 1407
Size: 1038 369
SanDI GG'GwC_CC
Cuts at: 0 1021 1407
Size: 1021 386
SmlI C'TyrA_G
Cuts at: 0 439 714 1407
Size: 439 275 693
Fragments arranged by size:
693 439 275
SphI G_CATG'C
Cuts at: 0 1050 1407
Size: 1050 357
StyI C'CwwG_G
Cuts at: 0 401 598 782 817 1014 1083 1340 1407
Size: 401 197 184 35 197 69 257 67
Fragments arranged by size:
401 257 197 197 184 69 67 35
TatI w'GTAC_w
Cuts at: 0 641 1347 1407
Size: 641 706 60
Fragments arranged by size:
706 641 60
Tth111I GACn'n_nGTC
Cuts at: 0 723 1407
Size: 723 684
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1156 1387 1407
Size: 1156 231 20
Fragments arranged by size:
1156 231 20
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI ApaI ApaLI ApoI AvrII
BanI BanII BbsI BglI Bme1580I BmrI BpuEI BsaHI
BseRI BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspHI
BsrDI BsrFI BstBI BstEII Bsu36I BtgI BtgZI BtsI
ClaI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI Hin4I HincII HindIII HpaI KpnI
MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI
PciI PflMI PpuMI PshAI PsiI PspOMI PvuII SacI
SacII SanDI SmlI SphI StyI TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AscI AseI AsiSI AvaI BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BplI BpmI Bpu10I BsaI BsaAI
BsaBI BsaWI BsaXI BsiEI BsmI BspEI BspMI BsrBI
BsrGI BssHII BssSI BstAPI BstXI BstYI BstZ17I CspCI
DraI DraIII DrdI EagI FseI FspI FspAI HaeII
KasI MfeI MluI MscI NarI NheI NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PspXI
PsrI PstI PvuI RsrII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TaqII TstI XbaI
XhoI XmaI XmnI ZraI