Restriction map for
pF1KB8440
(Linear) (Six-base) MAPSORT of: KIBB8440.seq Check: 3 from: 1 to: 1263
KIBB8440.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 712 1263
Size: 712 551
Acc65I G'GTAC_C
Cuts at: 0 206 1263
Size: 206 1057
AflIII A'CryG_T
Cuts at: 0 70 214 597 1263
Size: 70 144 383 666
Fragments arranged by size:
666 383 144 70
AleI CACnn'nnGTG
Cuts at: 0 215 1263
Size: 215 1048
ApoI r'AATT_y
Cuts at: 0 234 1263
Size: 234 1029
BanI G'GyrC_C
Cuts at: 0 206 351 1263
Size: 206 145 912
Fragments arranged by size:
912 206 145
BanII G_rGCy'C
Cuts at: 0 1241 1263
Size: 1241 22
BbeI G_GCGC'C
Cuts at: 0 355 1263
Size: 355 908
BbsI GAAGACnn'nnnn_
Cuts at: 0 1198 1263
Size: 1198 65
BciVI GTATCCnnnnn_n'
Cuts at: 0 692 1263
Size: 692 571
BglII A'GATC_T
Cuts at: 0 182 1263
Size: 182 1081
Bme1580I G_kGCm'C
Cuts at: 0 801 1263
Size: 801 462
BmrI ACTGGGnnnn_n'
Cuts at: 0 630 1263
Size: 630 633
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 315 1052 1263
Size: 163 152 737 211
Fragments arranged by size:
737 211 163 152
BsaI GGTCTCn'nnnn_
Cuts at: 0 1222 1263
Size: 1222 41
BsaAI yAC'GTr
Cuts at: 0 71 191 598 1263
Size: 71 120 407 665
Fragments arranged by size:
665 407 120 71
BsaHI Gr'CG_yC
Cuts at: 0 352 1263
Size: 352 911
BseYI C'CCAG_C
Cuts at: 0 530 542 782 1025 1263
Size: 530 12 240 243 238
Fragments arranged by size:
530 243 240 238 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 1263
Size: 128 1135
BsiEI CG_ry'CG
Cuts at: 0 925 1263
Size: 925 338
BsiHKAI G_wGCw'C
Cuts at: 0 251 1241 1263
Size: 251 990 22
Fragments arranged by size:
990 251 22
Bsp1286I G_dGCh'C
Cuts at: 0 251 801 1241 1263
Size: 251 550 440 22
Fragments arranged by size:
550 440 251 22
BspHI T'CATG_A
Cuts at: 0 390 1263
Size: 390 873
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 712 1263
Size: 712 551
BsrDI GCAATG_nn'
Cuts at: 0 425 1263
Size: 425 838
BsrFI r'CCGG_y
Cuts at: 0 538 1017 1263
Size: 538 479 246
Fragments arranged by size:
538 479 246
BssSI C'ACGA_G
Cuts at: 0 433 1263
Size: 433 830
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 217 1263
Size: 30 187 1046
Fragments arranged by size:
1046 187 30
BstYI r'GATC_y
Cuts at: 0 182 603 1263
Size: 182 421 660
Fragments arranged by size:
660 421 182
BtgI C'CryG_G
Cuts at: 0 47 1263
Size: 47 1216
BtsI GCAGTG_nn'
Cuts at: 0 47 251 261 1263
Size: 47 204 10 1002
Fragments arranged by size:
1002 204 47 10
DraIII CAC_nnn'GTG
Cuts at: 0 115 1263
Size: 115 1148
EaeI y'GGCC_r
Cuts at: 0 536 827 896 1263
Size: 536 291 69 367
Fragments arranged by size:
536 367 291 69
EarI CTCTTCn'nnn_
Cuts at: 0 551 1263
Size: 551 712
EcoICRI GAG'CTC
Cuts at: 0 1239 1263
Size: 1239 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 315 1052 1263
Size: 163 152 737 211
Fragments arranged by size:
737 211 163 152
EcoO109I rG'GnC_Cy
Cuts at: 0 155 1263
Size: 155 1108
EcoRI G'AATT_C
Cuts at: 0 234 1263
Size: 234 1029
HaeII r_GCGC'y
Cuts at: 0 355 468 758 813 1161 1263
Size: 355 113 290 55 348 102
Fragments arranged by size:
355 348 290 113 102 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 233 265 432 464 1263
Size: 233 32 167 32 799
Fragments arranged by size:
799 233 167 32 32
KasI G'GCGC_C
Cuts at: 0 351 1263
Size: 351 912
KpnI G_GTAC'C
Cuts at: 0 210 1263
Size: 210 1053
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1008 1226 1263
Size: 1008 218 37
Fragments arranged by size:
1008 218 37
MslI CAynn'nnrTG
Cuts at: 0 215 1263
Size: 215 1048
MspA1I CmG'CkG
Cuts at: 0 719 1263
Size: 719 544
NaeI GCC'GGC
Cuts at: 0 540 1263
Size: 540 723
NarI GG'CG_CC
Cuts at: 0 352 1263
Size: 352 911
NdeI CA'TA_TG
Cuts at: 0 327 1263
Size: 327 936
NgoMIV G'CCGG_C
Cuts at: 0 538 1263
Size: 538 725
NspI r_CATG'y
Cuts at: 0 218 1263
Size: 218 1045
PciI A'CATG_T
Cuts at: 0 214 1263
Size: 214 1049
PflMI CCAn_nnn'nTGG
Cuts at: 0 115 1263
Size: 115 1148
PmlI CAC'GTG
Cuts at: 0 71 598 1263
Size: 71 527 665
Fragments arranged by size:
665 527 71
SacI G_AGCT'C
Cuts at: 0 1241 1263
Size: 1241 22
SfcI C'TryA_G
Cuts at: 0 686 1162 1263
Size: 686 476 101
Fragments arranged by size:
686 476 101
SfoI GGC'GCC
Cuts at: 0 353 1263
Size: 353 910
StuI AGG'CCT
Cuts at: 0 506 1263
Size: 506 757
StyI C'CwwG_G
Cuts at: 0 973 1137 1263
Size: 973 164 126
Fragments arranged by size:
973 164 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 234 522 1263
Size: 234 288 741
Fragments arranged by size:
741 288 234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1116 1148 1263
Size: 1116 32 115
Fragments arranged by size:
1116 115 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 643 1263
Size: 643 620
Enzymes that do cut:
AarI Acc65I AflIII AleI ApoI BanI BanII BbeI
BbsI BciVI BglII Bme1580I BmrI BpmI BsaI BsaAI
BsaHI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrDI BsrFI BssSI BstXI BstYI BtgI BtsI DraIII
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I
KasI KpnI MmeI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PflMI PmlI SacI SfcI SfoI
StuI StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbvCI BcgI BclI BfrBI
BglI BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaBI
BsaWI BsaXI BseRI BsiWI BsmI BsmBI BspEI BsrBI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EciI EcoNI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI MfeI
MluI MscI NcoI NheI NotI NruI NsiI PacI
PasI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TatI Tth111I XbaI XhoI XmaI XmnI ZraI