Restriction map for
pF1KB8437
(Linear) (Six-base) MAPSORT of: KIBB8437.seq Check: 3688 from: 1 to: 1578
KIBB8437.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 1042 1224 1578
Size: 171 871 182 354
Fragments arranged by size:
871 354 182 171
AfeI AGC'GCT
Cuts at: 0 184 205 1578
Size: 184 21 1373
Fragments arranged by size:
1373 184 21
AflIII A'CryG_T
Cuts at: 0 585 1578
Size: 585 993
AhdI GACnn_n'nnGTC
Cuts at: 0 1473 1578
Size: 1473 105
ApoI r'AATT_y
Cuts at: 0 494 759 1578
Size: 494 265 819
Fragments arranged by size:
819 494 265
AvaI C'yCGr_G
Cuts at: 0 128 279 995 1082 1578
Size: 128 151 716 87 496
Fragments arranged by size:
716 496 151 128 87
BamHI G'GATC_C
Cuts at: 0 1261 1578
Size: 1261 317
BanII G_rGCy'C
Cuts at: 0 539 1578
Size: 539 1039
BbsI GAAGACnn'nnnn_
Cuts at: 0 410 422 1166 1578
Size: 410 12 744 412
Fragments arranged by size:
744 412 410 12
BbvCI CC'TCA_GC
Cuts at: 0 92 622 1578
Size: 92 530 956
Fragments arranged by size:
956 530 92
BclI T'GATC_A
Cuts at: 0 1076 1578
Size: 1076 502
BglII A'GATC_T
Cuts at: 0 750 1578
Size: 750 828
BmgBI CAC'GTC
Cuts at: 0 137 1578
Size: 137 1441
BmtI G_CTAG'C
Cuts at: 0 145 1578
Size: 145 1433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1150 1578
Size: 76 1074 428
Fragments arranged by size:
1074 428 76
Bpu10I CC'TnA_GC
Cuts at: 0 92 622 1578
Size: 92 530 956
Fragments arranged by size:
956 530 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 840 1468 1578
Size: 840 628 110
Fragments arranged by size:
840 628 110
BsaI GGTCTCn'nnnn_
Cuts at: 0 310 1578
Size: 310 1268
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 1578
Size: 109 1469
BseYI C'CCAG_C
Cuts at: 0 1185 1372 1578
Size: 1185 187 206
Fragments arranged by size:
1185 206 187
BsiHKAI G_wGCw'C
Cuts at: 0 286 539 1578
Size: 286 253 1039
Fragments arranged by size:
1039 286 253
BsmI GAATG_Cn'
Cuts at: 0 931 1207 1578
Size: 931 276 371
Fragments arranged by size:
931 371 276
Bsp1286I G_dGCh'C
Cuts at: 0 286 539 1578
Size: 286 253 1039
Fragments arranged by size:
1039 286 253
BspHI T'CATG_A
Cuts at: 0 444 1578
Size: 444 1134
BsrBI CCG'CTC
Cuts at: 0 945 1578
Size: 945 633
BssSI C'ACGA_G
Cuts at: 0 601 1494 1578
Size: 601 893 84
Fragments arranged by size:
893 601 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 603 1578
Size: 603 975
BstYI r'GATC_y
Cuts at: 0 750 1261 1578
Size: 750 511 317
Fragments arranged by size:
750 511 317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 732 1578
Size: 732 846
BtsI GCAGTG_nn'
Cuts at: 0 228 1321 1578
Size: 228 1093 257
Fragments arranged by size:
1093 257 228
EaeI y'GGCC_r
Cuts at: 0 5 68 80 1578
Size: 5 63 12 1498
Fragments arranged by size:
1498 63 12 5
EarI CTCTTCn'nnn_
Cuts at: 0 527 1545 1578
Size: 527 1018 33
Fragments arranged by size:
1018 527 33
EcoICRI GAG'CTC
Cuts at: 0 537 1578
Size: 537 1041
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 171 1042 1150 1224 1578
Size: 76 95 871 108 74 354
Fragments arranged by size:
871 354 108 95 76 74
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 556 964 1087 1578
Size: 556 408 123 491
Fragments arranged by size:
556 491 408 123
HaeII r_GCGC'y
Cuts at: 0 186 207 1578
Size: 186 21 1371
Fragments arranged by size:
1371 186 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1459 1491 1578
Size: 1459 32 87
Fragments arranged by size:
1459 87 32
MscI TGG'CCA
Cuts at: 0 7 1578
Size: 7 1571
MslI CAynn'nnrTG
Cuts at: 0 601 1578
Size: 601 977
MspA1I CmG'CkG
Cuts at: 0 74 1119 1578
Size: 74 1045 459
Fragments arranged by size:
1045 459 74
NheI G'CTAG_C
Cuts at: 0 141 1578
Size: 141 1437
NspI r_CATG'y
Cuts at: 0 589 1051 1578
Size: 589 462 527
Fragments arranged by size:
589 527 462
PciI A'CATG_T
Cuts at: 0 585 1578
Size: 585 993
PfoI T'CCnGG_A
Cuts at: 0 701 1166 1578
Size: 701 465 412
Fragments arranged by size:
701 465 412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1381 1413 1578
Size: 1381 32 165
Fragments arranged by size:
1381 165 32
PshAI GACnn'nnGTC
Cuts at: 0 258 1578
Size: 258 1320
PsiI TTA'TAA
Cuts at: 0 938 1578
Size: 938 640
PspXI vC'TCGA_Gb
Cuts at: 0 279 1082 1578
Size: 279 803 496
Fragments arranged by size:
803 496 279
PstI C_TGCA'G
Cuts at: 0 52 203 646 1578
Size: 52 151 443 932
Fragments arranged by size:
932 443 151 52
PvuII CAG'CTG
Cuts at: 0 1119 1578
Size: 1119 459
RsrII CG'GwC_CG
Cuts at: 0 76 1578
Size: 76 1502
SacI G_AGCT'C
Cuts at: 0 539 1578
Size: 539 1039
SapI GCTCTTCn'nnn_
Cuts at: 0 527 1578
Size: 527 1051
SfcI C'TryA_G
Cuts at: 0 48 199 642 712 1429 1482 1578
Size: 48 151 443 70 717 53 96
Fragments arranged by size:
717 443 151 96 70 53 48
SmlI C'TyrA_G
Cuts at: 0 279 819 995 1082 1447 1578
Size: 279 540 176 87 365 131
Fragments arranged by size:
540 365 279 176 131 87
StuI AGG'CCT
Cuts at: 0 439 1578
Size: 439 1139
StyI C'CwwG_G
Cuts at: 0 8 83 353 393 1578
Size: 8 75 270 40 1185
Fragments arranged by size:
1185 270 75 40 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1108 1177 1578
Size: 1108 69 401
Fragments arranged by size:
1108 401 69
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 669 1578
Size: 669 909
XhoI C'TCGA_G
Cuts at: 0 279 995 1082 1578
Size: 279 716 87 496
Fragments arranged by size:
716 496 279 87
Enzymes that do cut:
AcuI AfeI AflIII AhdI ApoI AvaI BamHI BanII
BbsI BbvCI BclI BglII BmgBI BmtI BpmI Bpu10I
BpuEI BsaI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI
BsrBI BssSI BstXI BstYI BtgZI BtsI EaeI EarI
EcoICRI Eco57MI FalI HaeII Hin4I MscI MslI MspA1I
NheI NspI PciI PfoI PpiI PshAI PsiI PspXI
PstI PvuII RsrII SacI SapI SfcI SmlI StuI
StyI TaqII XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BbeI BcgI BciVI BfrBI BglI
BlpI Bme1580I BmrI BplI BsaAI BsaBI BsaHI BsaWI
BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpuMI PspOMI PsrI PvuI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI Tth111I XbaI XmaI XmnI ZraI