Restriction map for
pF1KB8436
(Linear) (Six-base) MAPSORT of: KIBB8436.seq Check: 9907 from: 1 to: 1077
KIBB8436.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AccI GT'mk_AC
Cuts at: 0 866 1077
Size: 866 211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1077
Size: 949 128
AfeI AGC'GCT
Cuts at: 0 692 1077
Size: 692 385
ApoI r'AATT_y
Cuts at: 0 919 1077
Size: 919 158
AvaI C'yCGr_G
Cuts at: 0 133 914 1077
Size: 133 781 163
Fragments arranged by size:
781 163 133
BanII G_rGCy'C
Cuts at: 0 119 1077
Size: 119 958
BciVI GTATCCnnnnn_n'
Cuts at: 0 190 641 1077
Size: 190 451 436
Fragments arranged by size:
451 436 190
BclI T'GATC_A
Cuts at: 0 269 705 1077
Size: 269 436 372
Fragments arranged by size:
436 372 269
BglI GCCn_nnn'nGGC
Cuts at: 0 262 1077
Size: 262 815
BglII A'GATC_T
Cuts at: 0 828 1077
Size: 828 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 1030 1077
Size: 28 1002 47
Fragments arranged by size:
1002 47 28
Bpu10I CC'TnA_GC
Cuts at: 0 31 1077
Size: 31 1046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 274 1077
Size: 103 171 803
Fragments arranged by size:
803 171 103
BsaAI yAC'GTr
Cuts at: 0 986 1077
Size: 986 91
BsaBI GATnn'nnATC
Cuts at: 0 178 1077
Size: 178 899
BsaHI Gr'CG_yC
Cuts at: 0 104 1077
Size: 104 973
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 346 376 1077
Size: 346 30 701
Fragments arranged by size:
701 346 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 789 853 1077
Size: 52 737 64 224
Fragments arranged by size:
737 224 64 52
BsiHKAI G_wGCw'C
Cuts at: 0 119 314 1077
Size: 119 195 763
Fragments arranged by size:
763 195 119
BsmI GAATG_Cn'
Cuts at: 0 433 1077
Size: 433 644
Bsp1286I G_dGCh'C
Cuts at: 0 119 314 1077
Size: 119 195 763
Fragments arranged by size:
763 195 119
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 524 1077
Size: 524 553
BsrBI CCG'CTC
Cuts at: 0 78 1077
Size: 78 999
BssSI C'ACGA_G
Cuts at: 0 715 1077
Size: 715 362
BstYI r'GATC_y
Cuts at: 0 293 828 1077
Size: 293 535 249
Fragments arranged by size:
535 293 249
BtgI C'CryG_G
Cuts at: 0 186 278 1077
Size: 186 92 799
Fragments arranged by size:
799 186 92
BtsI GCAGTG_nn'
Cuts at: 0 651 1077
Size: 651 426
DrdI GACnn_nn'nnGTC
Cuts at: 0 9 1077
Size: 9 1068
EaeI y'GGCC_r
Cuts at: 0 254 1077
Size: 254 823
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 72 1077
Size: 72 1005
EcoICRI GAG'CTC
Cuts at: 0 117 1077
Size: 117 960
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 949 1030 1077
Size: 28 921 81 47
Fragments arranged by size:
921 81 47 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 34 1077
Size: 34 1043
EcoRI G'AATT_C
Cuts at: 0 919 1077
Size: 919 158
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 553 777 1077
Size: 553 224 300
Fragments arranged by size:
553 300 224
HaeII r_GCGC'y
Cuts at: 0 694 1077
Size: 694 383
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 346 378 533 565 1077
Size: 346 32 155 32 512
Fragments arranged by size:
512 346 155 32 32
HincII GTy'rAC
Cuts at: 0 867 1077
Size: 867 210
MslI CAynn'nnrTG
Cuts at: 0 256 1077
Size: 256 821
NcoI C'CATG_G
Cuts at: 0 186 278 1077
Size: 186 92 799
Fragments arranged by size:
799 186 92
NdeI CA'TA_TG
Cuts at: 0 381 1077
Size: 381 696
PfoI T'CCnGG_A
Cuts at: 0 414 422 1077
Size: 414 8 655
Fragments arranged by size:
655 414 8
PmlI CAC'GTG
Cuts at: 0 986 1077
Size: 986 91
SacI G_AGCT'C
Cuts at: 0 119 1077
Size: 119 958
SalI G'TCGA_C
Cuts at: 0 865 1077
Size: 865 212
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 262 1077
Size: 262 815
SmlI C'TyrA_G
Cuts at: 0 118 289 1077
Size: 118 171 788
Fragments arranged by size:
788 171 118
StyI C'CwwG_G
Cuts at: 0 44 186 278 1077
Size: 44 142 92 799
Fragments arranged by size:
799 142 92 44
TatI w'GTAC_w
Cuts at: 0 1064 1077
Size: 1064 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 278 310 1077
Size: 278 32 767
Fragments arranged by size:
767 278 32
XbaI T'CTAG_A
Cuts at: 0 834 1077
Size: 834 243
XmnI GAAnn'nnTTC
Cuts at: 0 247 923 934 1077
Size: 247 676 11 143
Fragments arranged by size:
676 247 143 11
Enzymes that do cut:
AccI AcuI AfeI ApoI AvaI BanII BciVI BclI
BglI BglII BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaXI BseRI BsiHKAI BsmI Bsp1286I BspMI BsrBI BssSI
BstYI BtgI BtsI DrdI EaeI EciI EcoICRI Eco57MI
EcoNI EcoRI FalI HaeII Hin4I HincII MslI NcoI
NdeI PfoI PmlI SacI SalI SfiI SmlI StyI
TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BfrBI BlpI Bme1580I BmgBI BmrI BmtI BplI
BsaI BsaWI BseYI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EagI EarI EcoO109I EcoRV FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I
NaeI NarI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacII SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII Tth111I XcmI XhoI XmaI ZraI