Restriction map for
pF1KB8435
(Linear) (Six-base) MAPSORT of: KIBB8435.seq Check: 491 from: 1 to: 1509
KIBB8435.orf
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 749 820 1241 1509
Size: 223 526 71 421 268
Fragments arranged by size:
526 421 268 223 71
AflIII A'CryG_T
Cuts at: 0 1326 1509
Size: 1326 183
AhdI GACnn_n'nnGTC
Cuts at: 0 91 699 1509
Size: 91 608 810
Fragments arranged by size:
810 608 91
AleI CACnn'nnGTG
Cuts at: 0 10 1509
Size: 10 1499
AlwNI CAG_nnn'CTG
Cuts at: 0 171 344 360 756 1509
Size: 171 173 16 396 753
Fragments arranged by size:
753 396 173 171 16
ApaLI G'TGCA_C
Cuts at: 0 1330 1468 1509
Size: 1330 138 41
Fragments arranged by size:
1330 138 41
ApoI r'AATT_y
Cuts at: 0 936 1509
Size: 936 573
AscI GG'CGCG_CC
Cuts at: 0 1487 1509
Size: 1487 22
AvaI C'yCGr_G
Cuts at: 0 1482 1509
Size: 1482 27
BanI G'GyrC_C
Cuts at: 0 685 1439 1509
Size: 685 754 70
Fragments arranged by size:
754 685 70
BanII G_rGCy'C
Cuts at: 0 293 1509
Size: 293 1216
BbsI GAAGACnn'nnnn_
Cuts at: 0 217 258 305 1055 1103 1509
Size: 217 41 47 750 48 406
Fragments arranged by size:
750 406 217 48 47 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 316 350 1509
Size: 316 34 1159
Fragments arranged by size:
1159 316 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 358 1509
Size: 358 1151
BglI GCCn_nnn'nGGC
Cuts at: 0 793 1451 1509
Size: 793 658 58
Fragments arranged by size:
793 658 58
Bme1580I G_kGCm'C
Cuts at: 0 1334 1472 1509
Size: 1334 138 37
Fragments arranged by size:
1334 138 37
BmgBI CAC'GTC
Cuts at: 0 1335 1509
Size: 1335 174
BmrI ACTGGGnnnn_n'
Cuts at: 0 1237 1509
Size: 1237 272
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 200 928 1509
Size: 200 728 581
Fragments arranged by size:
728 581 200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 382 1078 1509
Size: 193 189 696 431
Fragments arranged by size:
696 431 193 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 883 1509
Size: 394 489 626
Fragments arranged by size:
626 489 394
BsaI GGTCTCn'nnnn_
Cuts at: 0 398 1420 1509
Size: 398 1022 89
Fragments arranged by size:
1022 398 89
BsaAI yAC'GTr
Cuts at: 0 1329 1509
Size: 1329 180
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 223 253 708 738 1029 1059 1509
Size: 223 30 455 30 291 30 450
Fragments arranged by size:
455 450 291 223 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 259 1438 1509
Size: 259 1179 71
Fragments arranged by size:
1179 259 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 87 511 1034 1509
Size: 3 84 424 523 475
Fragments arranged by size:
523 475 424 84 3
BsiHKAI G_wGCw'C
Cuts at: 0 293 1334 1472 1509
Size: 293 1041 138 37
Fragments arranged by size:
1041 293 138 37
BsmI GAATG_Cn'
Cuts at: 0 207 1509
Size: 207 1302
Bsp1286I G_dGCh'C
Cuts at: 0 293 1334 1472 1509
Size: 293 1041 138 37
Fragments arranged by size:
1041 293 138 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 25 431 1286 1509
Size: 25 406 855 223
Fragments arranged by size:
855 406 223 25
BsrFI r'CCGG_y
Cuts at: 0 106 1400 1509
Size: 106 1294 109
Fragments arranged by size:
1294 109 106
BsrGI T'GTAC_A
Cuts at: 0 611 1509
Size: 611 898
BssHII G'CGCG_C
Cuts at: 0 1487 1509
Size: 1487 22
BssSI C'ACGA_G
Cuts at: 0 622 1509
Size: 622 887
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1467 1509
Size: 1467 42
BstEII G'GTnAC_C
Cuts at: 0 12 1509
Size: 12 1497
BstXI CCAn_nnnn'nTGG
Cuts at: 0 641 924 1509
Size: 641 283 585
Fragments arranged by size:
641 585 283
BstYI r'GATC_y
Cuts at: 0 188 356 1509
Size: 188 168 1153
Fragments arranged by size:
1153 188 168
Bsu36I CC'TnA_GG
Cuts at: 0 548 1509
Size: 548 961
BtgI C'CryG_G
Cuts at: 0 8 808 827 1133 1509
Size: 8 800 19 306 376
Fragments arranged by size:
800 376 306 19 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1344 1509
Size: 1344 165
BtsI GCAGTG_nn'
Cuts at: 0 461 1476 1509
Size: 461 1015 33
Fragments arranged by size:
1015 461 33
EaeI y'GGCC_r
Cuts at: 0 2 108 431 926 1283 1509
Size: 2 106 323 495 357 226
Fragments arranged by size:
495 357 323 226 106 2
EcoICRI GAG'CTC
Cuts at: 0 291 1509
Size: 291 1218
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 223 382 749 820 1078 1241 1509
Size: 193 30 159 367 71 258 163 268
Fragments arranged by size:
367 268 258 193 163 159 71 30
EcoO109I rG'GnC_Cy
Cuts at: 0 552 795 1509
Size: 552 243 714
Fragments arranged by size:
714 552 243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1057 1509
Size: 1057 452
FseI GG_CCGG'CC
Cuts at: 0 1404 1509
Size: 1404 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 77 109 223 255 685 717 1029 1061
Size: 77 32 114 32 430 32 312 32
Cuts at: 1061 1509
Size: 448
Fragments arranged by size:
448 430 312 114 77 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1158 1509
Size: 1158 351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 236 1509
Size: 108 128 1273
Fragments arranged by size:
1273 128 108
MscI TGG'CCA
Cuts at: 0 4 433 928 1285 1509
Size: 4 429 495 357 224
Fragments arranged by size:
495 429 357 224 4
MslI CAynn'nnrTG
Cuts at: 0 10 116 919 1509
Size: 10 106 803 590
Fragments arranged by size:
803 590 106 10
MspA1I CmG'CkG
Cuts at: 0 1503 1509
Size: 1503 6
NaeI GCC'GGC
Cuts at: 0 1402 1509
Size: 1402 107
NgoMIV G'CCGG_C
Cuts at: 0 1400 1509
Size: 1400 109
NspI r_CATG'y
Cuts at: 0 599 1509
Size: 599 910
PasI CC'CwG_GG
Cuts at: 0 1198 1509
Size: 1198 311
PflMI CCAn_nnn'nTGG
Cuts at: 0 171 261 1509
Size: 171 90 1248
Fragments arranged by size:
1248 171 90
PfoI T'CCnGG_A
Cuts at: 0 359 1509
Size: 359 1150
PmlI CAC'GTG
Cuts at: 0 1329 1509
Size: 1329 180
PpuMI rG'GwC_Cy
Cuts at: 0 552 1509
Size: 552 957
PstI C_TGCA'G
Cuts at: 0 456 1508 1509
Size: 456 1052 1
Fragments arranged by size:
1052 456 1
SacI G_AGCT'C
Cuts at: 0 293 1509
Size: 293 1216
ScaI AGT'ACT
Cuts at: 0 319 1509
Size: 319 1190
SexAI A'CCwGG_T
Cuts at: 0 260 1235 1509
Size: 260 975 274
Fragments arranged by size:
975 274 260
SfcI C'TryA_G
Cuts at: 0 321 452 1504 1509
Size: 321 131 1052 5
Fragments arranged by size:
1052 321 131 5
SmlI C'TyrA_G
Cuts at: 0 409 898 1509
Size: 409 489 611
Fragments arranged by size:
611 489 409
StuI AGG'CCT
Cuts at: 0 1177 1509
Size: 1177 332
TatI w'GTAC_w
Cuts at: 0 317 611 1382 1509
Size: 317 294 771 127
Fragments arranged by size:
771 317 294 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 708 740 1509
Size: 708 32 769
Fragments arranged by size:
769 708 32
Tth111I GACn'n_nGTC
Cuts at: 0 718 1509
Size: 718 791
XmnI GAAnn'nnTTC
Cuts at: 0 877 1509
Size: 877 632
Enzymes that do cut:
AcuI AflIII AhdI AleI AlwNI ApaLI ApoI AscI
AvaI BanI BanII BbsI BcgI BciVI BglI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaXI
BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI EaeI EcoICRI Eco57MI EcoO109I FalI FseI
Hin4I HincII MmeI MscI MslI MspA1I NaeI NgoMIV
NspI PasI PflMI PfoI PmlI PpuMI PstI SacI
ScaI SexAI SfcI SmlI StuI TatI TstI Tth111I
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI AloI ApaI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BclI BfrBI BglII BlpI BmtI Bpu10I
BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BstBI BstZ17I ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoNI EcoRI EcoRV
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StyI SwaI TaqII XbaI XcmI
XhoI XmaI ZraI