Restriction map for
pF1KB8413
(Linear) (Six-base) MAPSORT of: KIBB8413.seq Check: 8355 from: 1 to: 2160
KIBB8413.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 751 2160
Size: 553 198 1409
Fragments arranged by size:
1409 553 198
AflIII A'CryG_T
Cuts at: 0 557 2006 2160
Size: 557 1449 154
Fragments arranged by size:
1449 557 154
AhdI GACnn_n'nnGTC
Cuts at: 0 978 1563 2160
Size: 978 585 597
Fragments arranged by size:
978 597 585
AleI CACnn'nnGTG
Cuts at: 0 428 1742 2160
Size: 428 1314 418
Fragments arranged by size:
1314 428 418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 2160
Size: 397 1763
AlwNI CAG_nnn'CTG
Cuts at: 0 618 982 1635 2160
Size: 618 364 653 525
Fragments arranged by size:
653 618 525 364
ApoI r'AATT_y
Cuts at: 0 1799 2160
Size: 1799 361
AseI AT'TA_AT
Cuts at: 0 716 2160
Size: 716 1444
AvaI C'yCGr_G
Cuts at: 0 245 1445 1603 2160
Size: 245 1200 158 557
Fragments arranged by size:
1200 557 245 158
BanII G_rGCy'C
Cuts at: 0 470 1238 1343 2129 2160
Size: 470 768 105 786 31
Fragments arranged by size:
786 768 470 105 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 1202 2160
Size: 1202 958
BbvCI CC'TCA_GC
Cuts at: 0 170 1585 2160
Size: 170 1415 575
Fragments arranged by size:
1415 575 170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1426 1460 2160
Size: 1426 34 700
Fragments arranged by size:
1426 700 34
BclI T'GATC_A
Cuts at: 0 1619 2160
Size: 1619 541
BfrBI ATG'CAT
Cuts at: 0 769 2160
Size: 769 1391
BglI GCCn_nnn'nGGC
Cuts at: 0 1217 2160
Size: 1217 943
BlpI GC'TnA_GC
Cuts at: 0 2047 2160
Size: 2047 113
Bme1580I G_kGCm'C
Cuts at: 0 1992 2160
Size: 1992 168
BmgBI CAC'GTC
Cuts at: 0 339 558 2160
Size: 339 219 1602
Fragments arranged by size:
1602 339 219
BmrI ACTGGGnnnn_n'
Cuts at: 0 270 1841 2160
Size: 270 1571 319
Fragments arranged by size:
1571 319 270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 655 2101 2160
Size: 610 45 1446 59
Fragments arranged by size:
1446 610 59 45
Bpu10I CC'TnA_GC
Cuts at: 0 170 1585 2160
Size: 170 1415 575
Fragments arranged by size:
1415 575 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1633 2160
Size: 1633 527
BsaI GGTCTCn'nnnn_
Cuts at: 0 286 681 1921 2160
Size: 286 395 1240 239
Fragments arranged by size:
1240 395 286 239
BsaWI w'CCGG_w
Cuts at: 0 788 1166 2160
Size: 788 378 994
Fragments arranged by size:
994 788 378
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1083 1113 2160
Size: 1083 30 1047
Fragments arranged by size:
1083 1047 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 972 1091 2160
Size: 972 119 1069
Fragments arranged by size:
1069 972 119
BseYI C'CCAG_C
Cuts at: 0 56 1053 2128 2160
Size: 56 997 1075 32
Fragments arranged by size:
1075 997 56 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 2160
Size: 236 1924
BsiHKAI G_wGCw'C
Cuts at: 0 470 1238 1343 1869 2160
Size: 470 768 105 526 291
Fragments arranged by size:
768 526 470 291 105
BsmI GAATG_Cn'
Cuts at: 0 442 1115 1213 2160
Size: 442 673 98 947
Fragments arranged by size:
947 673 442 98
Bsp1286I G_dGCh'C
Cuts at: 0 470 1238 1343 1869 1992 2129 2160
Size: 470 768 105 526 123 137 31
Fragments arranged by size:
768 526 470 137 123 105 31
BspEI T'CCGG_A
Cuts at: 0 1166 2160
Size: 1166 994
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1254 2160
Size: 1254 906
BsrDI GCAATG_nn'
Cuts at: 0 704 999 1299 2160
Size: 704 295 300 861
Fragments arranged by size:
861 704 300 295
BsrFI r'CCGG_y
Cuts at: 0 1640 1709 2160
Size: 1640 69 451
Fragments arranged by size:
1640 451 69
BsrGI T'GTAC_A
Cuts at: 0 176 1031 2160
Size: 176 855 1129
Fragments arranged by size:
1129 855 176
BssSI C'ACGA_G
Cuts at: 0 394 2160
Size: 394 1766
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1473 2160
Size: 1473 687
BstYI r'GATC_y
Cuts at: 0 1595 1607 2160
Size: 1595 12 553
Fragments arranged by size:
1595 553 12
Bsu36I CC'TnA_GG
Cuts at: 0 740 2160
Size: 740 1420
BtgI C'CryG_G
Cuts at: 0 477 548 647 1082 2160
Size: 477 71 99 435 1078
Fragments arranged by size:
1078 477 435 99 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1926 2160
Size: 1926 234
BtsI GCAGTG_nn'
Cuts at: 0 204 1230 2160
Size: 204 1026 930
Fragments arranged by size:
1026 930 204
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 377 412 2160
Size: 377 35 1748
Fragments arranged by size:
1748 377 35
EaeI y'GGCC_r
Cuts at: 0 29 40 320 581 1218 2160
Size: 29 11 280 261 637 942
Fragments arranged by size:
942 637 280 261 29 11
EarI CTCTTCn'nnn_
Cuts at: 0 1331 2160
Size: 1331 829
EcoICRI GAG'CTC
Cuts at: 0 468 1236 1341 2160
Size: 468 768 105 819
Fragments arranged by size:
819 768 468 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 610 655 751 2101 2160
Size: 553 57 45 96 1350 59
Fragments arranged by size:
1350 553 96 59 57 45
EcoO109I rG'GnC_Cy
Cuts at: 0 272 575 1731 2160
Size: 272 303 1156 429
Fragments arranged by size:
1156 429 303 272
HaeII r_GCGC'y
Cuts at: 0 1635 2160
Size: 1635 525
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 644 676 834 866 964 996
Size: 106 32 506 32 158 32 98 32
Cuts at: 996 1424 1456 1533 1549 1565 1581 2160
Size: 428 32 77 16 16 16 579
Fragments arranged by size:
579 506 428 158 106 98 77 32
32 32 32 32 16 16 16
MfeI C'AATT_G
Cuts at: 0 1574 2160
Size: 1574 586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 704 1871 2160
Size: 308 396 1167 289
Fragments arranged by size:
1167 396 308 289
MscI TGG'CCA
Cuts at: 0 31 42 322 583 2160
Size: 31 11 280 261 1577
Fragments arranged by size:
1577 280 261 31 11
MslI CAynn'nnrTG
Cuts at: 0 364 379 428 1243 1742 2056 2160
Size: 364 15 49 815 499 314 104
Fragments arranged by size:
815 499 364 314 104 49 15
MspA1I CmG'CkG
Cuts at: 0 174 261 1362 2160
Size: 174 87 1101 798
Fragments arranged by size:
1101 798 174 87
NcoI C'CATG_G
Cuts at: 0 477 548 1082 2160
Size: 477 71 534 1078
Fragments arranged by size:
1078 534 477 71
NsiI A_TGCA'T
Cuts at: 0 771 2160
Size: 771 1389
NspI r_CATG'y
Cuts at: 0 1131 2010 2160
Size: 1131 879 150
Fragments arranged by size:
1131 879 150
PasI CC'CwG_GG
Cuts at: 0 1000 2160
Size: 1000 1160
PciI A'CATG_T
Cuts at: 0 2006 2160
Size: 2006 154
PflMI CCAn_nnn'nTGG
Cuts at: 0 38 264 549 2160
Size: 38 226 285 1611
Fragments arranged by size:
1611 285 226 38
PfoI T'CCnGG_A
Cuts at: 0 587 1104 2072 2160
Size: 587 517 968 88
Fragments arranged by size:
968 587 517 88
PpuMI rG'GwC_Cy
Cuts at: 0 272 575 1731 2160
Size: 272 303 1156 429
Fragments arranged by size:
1156 429 303 272
PspXI vC'TCGA_Gb
Cuts at: 0 1445 2160
Size: 1445 715
PstI C_TGCA'G
Cuts at: 0 1265 1793 2160
Size: 1265 528 367
Fragments arranged by size:
1265 528 367
PvuII CAG'CTG
Cuts at: 0 174 261 1362 2160
Size: 174 87 1101 798
Fragments arranged by size:
1101 798 174 87
SacI G_AGCT'C
Cuts at: 0 470 1238 1343 2160
Size: 470 768 105 817
Fragments arranged by size:
817 768 470 105
SapI GCTCTTCn'nnn_
Cuts at: 0 1331 2160
Size: 1331 829
SbfI CC_TGCA'GG
Cuts at: 0 1265 1793 2160
Size: 1265 528 367
Fragments arranged by size:
1265 528 367
ScaI AGT'ACT
Cuts at: 0 1963 2160
Size: 1963 197
SfcI C'TryA_G
Cuts at: 0 426 1261 1789 2160
Size: 426 835 528 371
Fragments arranged by size:
835 528 426 371
SmaI CCC'GGG
Cuts at: 0 247 2160
Size: 247 1913
SmlI C'TyrA_G
Cuts at: 0 1445 1612 2160
Size: 1445 167 548
Fragments arranged by size:
1445 548 167
StyI C'CwwG_G
Cuts at: 0 477 548 1082 1118 2120 2160
Size: 477 71 534 36 1002 40
Fragments arranged by size:
1002 534 477 71 40 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 107 1027 1340 2160
Size: 107 920 313 820
Fragments arranged by size:
920 820 313 107
TatI w'GTAC_w
Cuts at: 0 176 1031 1133 1961 2160
Size: 176 855 102 828 199
Fragments arranged by size:
855 828 199 176 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2099 2131 2160
Size: 2099 32 29
Fragments arranged by size:
2099 32 29
Tth111I GACn'n_nGTC
Cuts at: 0 19 276 2143 2160
Size: 19 257 1867 17
Fragments arranged by size:
1867 257 19 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 315 1102 2031 2160
Size: 315 787 929 129
Fragments arranged by size:
929 787 315 129
XhoI C'TCGA_G
Cuts at: 0 1445 2160
Size: 1445 715
XmaI C'CCGG_G
Cuts at: 0 245 2160
Size: 245 1915
Enzymes that do cut:
AcuI AflIII AhdI AleI AlfI AlwNI ApoI AseI
AvaI BanII BbsI BbvCI BcgI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrDI BsrFI BsrGI BssSI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI EcoICRI
Eco57MI EcoO109I HaeII Hin4I MfeI MmeI MscI MslI
MspA1I NcoI NsiI NspI PasI PciI PflMI PfoI
PpuMI PspXI PstI PvuII SacI SapI SbfI ScaI
SfcI SmaI SmlI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BanI BbeI BciVI BglII BmtI BplI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BspHI BsrBI BssHII BstAPI
BstBI BstEII BstZ17I ClaI DraI DraIII DrdI EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacII
SalI SanDI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI XbaI XmnI
ZraI