Restriction map for
pF1KB8405
(Linear) (Six-base) MAPSORT of: KIBB8405.seq Check: 9506 from: 1 to: 2412
KIBB8405.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1008 2026 2412
Size: 1008 1018 386
Fragments arranged by size:
1018 1008 386
AccI GT'mk_AC
Cuts at: 0 1748 2412
Size: 1748 664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1757 1831 2412
Size: 1757 74 581
Fragments arranged by size:
1757 581 74
AflIII A'CryG_T
Cuts at: 0 114 2412
Size: 114 2298
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1010 2412
Size: 1010 1402
AlwNI CAG_nnn'CTG
Cuts at: 0 624 853 856 952 1158 1192 2412
Size: 624 229 3 96 206 34 1220
Fragments arranged by size:
1220 624 229 206 96 34 3
ApaI G_GGCC'C
Cuts at: 0 1580 1621 2412
Size: 1580 41 791
Fragments arranged by size:
1580 791 41
ApoI r'AATT_y
Cuts at: 0 1569 2412
Size: 1569 843
AseI AT'TA_AT
Cuts at: 0 761 2412
Size: 761 1651
BamHI G'GATC_C
Cuts at: 0 1309 2412
Size: 1309 1103
BanI G'GyrC_C
Cuts at: 0 1723 2412
Size: 1723 689
BanII G_rGCy'C
Cuts at: 0 1580 1621 2240 2412
Size: 1580 41 619 172
Fragments arranged by size:
1580 619 172 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 5 2281 2412
Size: 5 2276 131
Fragments arranged by size:
2276 131 5
BciVI GTATCCnnnnn_n'
Cuts at: 0 1401 2165 2412
Size: 1401 764 247
Fragments arranged by size:
1401 764 247
BclI T'GATC_A
Cuts at: 0 108 602 2309 2412
Size: 108 494 1707 103
Fragments arranged by size:
1707 494 108 103
BfrBI ATG'CAT
Cuts at: 0 1807 1866 2412
Size: 1807 59 546
Fragments arranged by size:
1807 546 59
BglI GCCn_nnn'nGGC
Cuts at: 0 1732 2395 2412
Size: 1732 663 17
Fragments arranged by size:
1732 663 17
BlpI GC'TnA_GC
Cuts at: 0 1001 1957 2412
Size: 1001 956 455
Fragments arranged by size:
1001 956 455
Bme1580I G_kGCm'C
Cuts at: 0 1580 1621 2412
Size: 1580 41 791
Fragments arranged by size:
1580 791 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 222 1097 1575 1624 2412
Size: 222 875 478 49 788
Fragments arranged by size:
875 788 478 222 49
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 474 2412
Size: 474 1938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 967 1049 2412
Size: 967 82 1363
Fragments arranged by size:
1363 967 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 445 1112 2412
Size: 69 376 667 1300
Fragments arranged by size:
1300 667 376 69
BsaI GGTCTCn'nnnn_
Cuts at: 0 1083 2078 2412
Size: 1083 995 334
Fragments arranged by size:
1083 995 334
BsaBI GATnn'nnATC
Cuts at: 0 2128 2412
Size: 2128 284
BsaHI Gr'CG_yC
Cuts at: 0 1757 2412
Size: 1757 655
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 1848 2412
Size: 184 1664 564
Fragments arranged by size:
1664 564 184
BseYI C'CCAG_C
Cuts at: 0 596 1230 2239 2412
Size: 596 634 1009 173
Fragments arranged by size:
1009 634 596 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 121 2412
Size: 65 56 2291
Fragments arranged by size:
2291 65 56
BsiEI CG_ry'CG
Cuts at: 0 1767 2008 2412
Size: 1767 241 404
Fragments arranged by size:
1767 404 241
BsiHKAI G_wGCw'C
Cuts at: 0 2263 2412
Size: 2263 149
BsmI GAATG_Cn'
Cuts at: 0 1807 1809 2412
Size: 1807 2 603
Fragments arranged by size:
1807 603 2
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1214 2412
Size: 1214 1198
Bsp1286I G_dGCh'C
Cuts at: 0 1580 1621 2240 2263 2412
Size: 1580 41 619 23 149
Fragments arranged by size:
1580 619 149 41 23
BspHI T'CATG_A
Cuts at: 0 809 2412
Size: 809 1603
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1008 2026 2412
Size: 1008 1018 386
Fragments arranged by size:
1018 1008 386
BsrBI CCG'CTC
Cuts at: 0 807 1000 2412
Size: 807 193 1412
Fragments arranged by size:
1412 807 193
BsrDI GCAATG_nn'
Cuts at: 0 71 1676 2412
Size: 71 1605 736
Fragments arranged by size:
1605 736 71
BssSI C'ACGA_G
Cuts at: 0 1742 2412
Size: 1742 670
BstAPI GCAn_nnn'nTGC
Cuts at: 0 741 853 856 952 2412
Size: 741 112 3 96 1460
Fragments arranged by size:
1460 741 112 96 3
BstYI r'GATC_y
Cuts at: 0 779 1309 1454 1894 1964 2412
Size: 779 530 145 440 70 448
Fragments arranged by size:
779 530 448 440 145 70
BtgI C'CryG_G
Cuts at: 0 1761 2412
Size: 1761 651
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2174 2412
Size: 2174 238
BtsI GCAGTG_nn'
Cuts at: 0 111 2382 2412
Size: 111 2271 30
Fragments arranged by size:
2271 111 30
DraIII CAC_nnn'GTG
Cuts at: 0 1489 2412
Size: 1489 923
EaeI y'GGCC_r
Cuts at: 0 1007 1642 1711 1764 2225 2412
Size: 1007 635 69 53 461 187
Fragments arranged by size:
1007 635 461 187 69 53
EagI C'GGCC_G
Cuts at: 0 1764 2412
Size: 1764 648
EarI CTCTTCn'nnn_
Cuts at: 0 92 883 1231 1299 1340 1937 2412
Size: 92 791 348 68 41 597 475
Fragments arranged by size:
791 597 475 348 92 68 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2300 2412
Size: 2300 112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 967 1049 1757 1831 2412
Size: 967 82 708 74 581
Fragments arranged by size:
967 708 581 82 74
EcoNI CCTnn'n_nnAGG
Cuts at: 0 317 2412
Size: 317 2095
EcoO109I rG'GnC_Cy
Cuts at: 0 1576 1577 2412
Size: 1576 1 835
Fragments arranged by size:
1576 835 1
EcoRV GAT'ATC
Cuts at: 0 1365 2412
Size: 1365 1047
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 29 403 1986 2412
Size: 29 374 1583 426
Fragments arranged by size:
1583 426 374 29
HaeII r_GCGC'y
Cuts at: 0 375 2412
Size: 375 2037
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 1354 1386 1439 1471 2058 2090
Size: 67 32 1255 32 53 32 587 32
Cuts at: 2090 2347 2379 2412
Size: 257 32 33
Fragments arranged by size:
1255 587 257 67 53 33 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1336 1494 1586 2412
Size: 1336 158 92 826
Fragments arranged by size:
1336 826 158 92
HindIII A'AGCT_T
Cuts at: 0 421 2038 2412
Size: 421 1617 374
Fragments arranged by size:
1617 421 374
HpaI GTT'AAC
Cuts at: 0 1586 2412
Size: 1586 826
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1898 1940 2412
Size: 1898 42 472
Fragments arranged by size:
1898 472 42
MscI TGG'CCA
Cuts at: 0 1009 1713 2412
Size: 1009 704 699
Fragments arranged by size:
1009 704 699
MslI CAynn'nnrTG
Cuts at: 0 1018 2412
Size: 1018 1394
MspA1I CmG'CkG
Cuts at: 0 49 600 837 853 949 1005 1461 1535
Size: 49 551 237 16 96 56 456 74
Cuts at: 1535 1763 2412
Size: 228 649
Fragments arranged by size:
649 551 456 237 228 96 74 56
49 16
NsiI A_TGCA'T
Cuts at: 0 1809 1868 2412
Size: 1809 59 544
Fragments arranged by size:
1809 544 59
NspI r_CATG'y
Cuts at: 0 118 801 1017 1866 2412
Size: 118 683 216 849 546
Fragments arranged by size:
849 683 546 216 118
PciI A'CATG_T
Cuts at: 0 114 2412
Size: 114 2298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 563 595 2412
Size: 563 32 1817
Fragments arranged by size:
1817 563 32
PspOMI G'GGCC_C
Cuts at: 0 1576 1617 2412
Size: 1576 41 795
Fragments arranged by size:
1576 795 41
PstI C_TGCA'G
Cuts at: 0 773 1540 1686 2412
Size: 773 767 146 726
Fragments arranged by size:
773 767 726 146
PvuII CAG'CTG
Cuts at: 0 49 600 853 949 1005 1535 2412
Size: 49 551 253 96 56 530 877
Fragments arranged by size:
877 551 530 253 96 56 49
SacII CC_GC'GG
Cuts at: 0 1764 2412
Size: 1764 648
SapI GCTCTTCn'nnn_
Cuts at: 0 1937 2412
Size: 1937 475
SexAI A'CCwGG_T
Cuts at: 0 903 2412
Size: 903 1509
SfcI C'TryA_G
Cuts at: 0 769 1536 1682 1693 1749 2370 2412
Size: 769 767 146 11 56 621 42
Fragments arranged by size:
769 767 621 146 56 42 11
SmlI C'TyrA_G
Cuts at: 0 84 424 1127 2412
Size: 84 340 703 1285
Fragments arranged by size:
1285 703 340 84
SphI G_CATG'C
Cuts at: 0 1017 2412
Size: 1017 1395
StuI AGG'CCT
Cuts at: 0 1852 2412
Size: 1852 560
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1231 2022 2412
Size: 1231 791 390
Fragments arranged by size:
1231 791 390
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1518 2263 2412
Size: 1518 745 149
Fragments arranged by size:
1518 745 149
XmnI GAAnn'nnTTC
Cuts at: 0 12 1225 1807 2412
Size: 12 1213 582 605
Fragments arranged by size:
1213 605 582 12
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI ApaI ApoI
AseI BamHI BanI BanII BbsI BciVI BclI BfrBI
BglI BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaBI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI
BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
HincII HindIII HpaI MmeI MscI MslI MspA1I NsiI
NspI PciI PpiI PspOMI PstI PvuII SacII SapI
SexAI SfcI SmlI SphI StuI TaqII XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaLI AscI AsiSI AvaI AvrII BaeI BbeI
BbvCI BcgI BglII BmgBI BmtI Bpu10I BsaAI BsaWI
BsaXI BsiWI BspEI BsrFI BsrGI BssHII BstBI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoICRI
EcoRI FseI FspI FspAI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PflMI PfoI PmeI PmlI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StyI SwaI TatI TstI Tth111I
XbaI XhoI XmaI ZraI