Restriction map for
pF1KB8404
(Linear) (Six-base) MAPSORT of: KIBB8404.seq Check: 154 from: 1 to: 3540
KIBB8404.orf
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 498 814 3540
Size: 498 316 2726
Fragments arranged by size:
2726 498 316
AccI GT'mk_AC
Cuts at: 0 287 1952 3540
Size: 287 1665 1588
Fragments arranged by size:
1665 1588 287
Acc65I G'GTAC_C
Cuts at: 0 652 3540
Size: 652 2888
AclI AA'CG_TT
Cuts at: 0 2534 3540
Size: 2534 1006
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3487 3540
Size: 3487 53
AflIII A'CryG_T
Cuts at: 0 846 1408 1937 2430 2563 3540
Size: 846 562 529 493 133 977
Fragments arranged by size:
977 846 562 529 493 133
AhdI GACnn_n'nnGTC
Cuts at: 0 1553 3540
Size: 1553 1987
AleI CACnn'nnGTG
Cuts at: 0 311 3010 3235 3540
Size: 311 2699 225 305
Fragments arranged by size:
2699 311 305 225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 221 329 2300 2931 3138 3540
Size: 221 108 1971 631 207 402
Fragments arranged by size:
1971 631 402 221 207 108
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 562 594 3540
Size: 562 32 2946
Fragments arranged by size:
2946 562 32
AlwNI CAG_nnn'CTG
Cuts at: 0 366 3085 3131 3540
Size: 366 2719 46 409
Fragments arranged by size:
2719 409 366 46
ApaI G_GGCC'C
Cuts at: 0 949 998 1154 2620 3271 3540
Size: 949 49 156 1466 651 269
Fragments arranged by size:
1466 949 651 269 156 49
AvaI C'yCGr_G
Cuts at: 0 1335 1973 2231 3271 3540
Size: 1335 638 258 1040 269
Fragments arranged by size:
1335 1040 638 269 258
BamHI G'GATC_C
Cuts at: 0 2834 3540
Size: 2834 706
BanI G'GyrC_C
Cuts at: 0 652 1237 1742 1825 2173 2496 2508 2595
Size: 652 585 505 83 348 323 12 87
Cuts at: 2595 2718 2846 3540
Size: 123 128 694
Fragments arranged by size:
694 652 585 505 348 323 128 123
87 83 12
BanII G_rGCy'C
Cuts at: 0 665 879 936 949 998 1154 1453 1893
Size: 665 214 57 13 49 156 299 440
Cuts at: 1893 2620 3148 3163 3190 3271 3540
Size: 727 528 15 27 81 269
Fragments arranged by size:
727 665 528 440 299 269 214 156
81 57 49 27 15 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 844 2449 3540
Size: 844 1605 1091
Fragments arranged by size:
1605 1091 844
BbvCI CC'TCA_GC
Cuts at: 0 1727 1913 2371 3540
Size: 1727 186 458 1169
Fragments arranged by size:
1727 1169 458 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 792 826 3313 3347 3485 3519 3540
Size: 792 34 2487 34 138 34 21
Fragments arranged by size:
2487 792 138 34 34 34 21
BciVI GTATCCnnnnn_n'
Cuts at: 0 1154 3540
Size: 1154 2386
BclI T'GATC_A
Cuts at: 0 1001 2789 3540
Size: 1001 1788 751
Fragments arranged by size:
1788 1001 751
BfrBI ATG'CAT
Cuts at: 0 2148 3525 3540
Size: 2148 1377 15
Fragments arranged by size:
2148 1377 15
BglI GCCn_nnn'nGGC
Cuts at: 0 2366 2727 3540
Size: 2366 361 813
Fragments arranged by size:
2366 813 361
BlpI GC'TnA_GC
Cuts at: 0 1790 3540
Size: 1790 1750
Bme1580I G_kGCm'C
Cuts at: 0 858 949 998 1154 2571 2600 2620 3262
Size: 858 91 49 156 1417 29 20 642
Cuts at: 3262 3271 3540
Size: 9 269
Fragments arranged by size:
1417 858 642 269 156 91 49 29
20 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 81 116 570 609 742 1096 1447 1512
Size: 81 35 454 39 133 354 351 65
Cuts at: 1512 1778 3540
Size: 266 1762
Fragments arranged by size:
1762 454 354 351 266 133 81 65
39 35
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 846 3540
Size: 846 2694
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 360 426 1003 1212 3132 3174 3390
Size: 89 271 66 577 209 1920 42 216
Cuts at: 3390 3540
Size: 150
Fragments arranged by size:
1920 577 271 216 209 150 89 66
42
Bpu10I CC'TnA_GC
Cuts at: 0 647 1727 1913 2371 3142 3540
Size: 647 1080 186 458 771 398
Fragments arranged by size:
1080 771 647 458 398 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 1528 3540
Size: 160 1368 2012
Fragments arranged by size:
2012 1368 160
BsaI GGTCTCn'nnnn_
Cuts at: 0 565 915 955 1041 2034 3540
Size: 565 350 40 86 993 1506
Fragments arranged by size:
1506 993 565 350 86 40
BsaAI yAC'GTr
Cuts at: 0 1938 2261 2433 2566 3008 3540
Size: 1938 323 172 133 442 532
Fragments arranged by size:
1938 532 442 323 172 133
BsaBI GATnn'nnATC
Cuts at: 0 2776 2794 3540
Size: 2776 18 746
Fragments arranged by size:
2776 746 18
BsaHI Gr'CG_yC
Cuts at: 0 223 2807 3540
Size: 223 2584 733
Fragments arranged by size:
2584 733 223
BsaWI w'CCGG_w
Cuts at: 0 28 525 879 3540
Size: 28 497 354 2661
Fragments arranged by size:
2661 497 354 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 562 592 2293 2323 3540
Size: 562 30 1701 30 1217
Fragments arranged by size:
1701 1217 562 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 490 1461 2782 3540
Size: 490 971 1321 758
Fragments arranged by size:
1321 971 758 490
BseYI C'CCAG_C
Cuts at: 0 161 639 1131 1386 1512 1885 2271 2411
Size: 161 478 492 255 126 373 386 140
Cuts at: 2411 2570 3018 3166 3540
Size: 159 448 148 374
Fragments arranged by size:
492 478 448 386 374 373 255 161
159 148 140 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 691 1154 3262 3540
Size: 335 356 463 2108 278
Fragments arranged by size:
2108 463 356 335 278
BsiEI CG_ry'CG
Cuts at: 0 54 3540
Size: 54 3486
BsiHKAI G_wGCw'C
Cuts at: 0 2321 3148 3540
Size: 2321 827 392
Fragments arranged by size:
2321 827 392
BsmI GAATG_Cn'
Cuts at: 0 2148 3540
Size: 2148 1392
Bsp1286I G_dGCh'C
Cuts at: 0 665 858 879 936 949 998 1154 1453
Size: 665 193 21 57 13 49 156 299
Cuts at: 1453 1893 2321 2571 2600 2620 3148 3163 3190
Size: 440 428 250 29 20 528 15 27
Cuts at: 3190 3262 3271 3540
Size: 72 9 269
Fragments arranged by size:
665 528 440 428 299 269 250 193
156 72 57 49 29 27 21 20
15 13 9
BspEI T'CCGG_A
Cuts at: 0 525 879 3540
Size: 525 354 2661
Fragments arranged by size:
2661 525 354
BspHI T'CATG_A
Cuts at: 0 1310 3540
Size: 1310 2230
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 498 814 3540
Size: 498 316 2726
Fragments arranged by size:
2726 498 316
BsrBI CCG'CTC
Cuts at: 0 115 3540
Size: 115 3425
BsrDI GCAATG_nn'
Cuts at: 0 48 1399 3540
Size: 48 1351 2141
Fragments arranged by size:
2141 1351 48
BsrFI r'CCGG_y
Cuts at: 0 49 2515 3540
Size: 49 2466 1025
Fragments arranged by size:
2466 1025 49
BssSI C'ACGA_G
Cuts at: 0 70 166 3540
Size: 70 96 3374
Fragments arranged by size:
3374 96 70
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3131 3540
Size: 3131 409
BstEII G'GTnAC_C
Cuts at: 0 726 1377 1461 3540
Size: 726 651 84 2079
Fragments arranged by size:
2079 726 651 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 129 3237 3540
Size: 129 3108 303
Fragments arranged by size:
3108 303 129
BstYI r'GATC_y
Cuts at: 0 1205 2771 2834 3383 3540
Size: 1205 1566 63 549 157
Fragments arranged by size:
1566 1205 549 157 63
Bsu36I CC'TnA_GG
Cuts at: 0 1286 1629 3540
Size: 1286 343 1911
Fragments arranged by size:
1911 1286 343
BtgI C'CryG_G
Cuts at: 0 1243 2424 2621 2811 2858 3540
Size: 1243 1181 197 190 47 682
Fragments arranged by size:
1243 1181 682 197 190 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1360 3076 3316 3540
Size: 1360 1716 240 224
Fragments arranged by size:
1716 1360 240 224
BtsI GCAGTG_nn'
Cuts at: 0 1880 2792 3284 3540
Size: 1880 912 492 256
Fragments arranged by size:
1880 912 492 256
DraIII CAC_nnn'GTG
Cuts at: 0 89 3540
Size: 89 3451
DrdI GACnn_nn'nnGTC
Cuts at: 0 598 891 1960 2937 3540
Size: 598 293 1069 977 603
Fragments arranged by size:
1069 977 603 598 293
EaeI y'GGCC_r
Cuts at: 0 51 386 1532 2048 2165 2517 2586 2643
Size: 51 335 1146 516 117 352 69 57
Cuts at: 2643 3347 3506 3540
Size: 704 159 34
Fragments arranged by size:
1146 704 516 352 335 159 117 69
57 51 34
EagI C'GGCC_G
Cuts at: 0 51 3540
Size: 51 3489
EarI CTCTTCn'nnn_
Cuts at: 0 524 1309 3540
Size: 524 785 2231
Fragments arranged by size:
2231 785 524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 487 2913 3282 3354 3376 3540
Size: 487 2426 369 72 22 164
Fragments arranged by size:
2426 487 369 164 72 22
EcoICRI GAG'CTC
Cuts at: 0 3146 3540
Size: 3146 394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 360 426 1003 1212 3132 3174 3390
Size: 89 271 66 577 209 1920 42 216
Cuts at: 3390 3487 3540
Size: 97 53
Fragments arranged by size:
1920 577 271 216 209 97 89 66
53 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 209 1324 3540
Size: 209 1115 2216
Fragments arranged by size:
2216 1115 209
EcoO109I rG'GnC_Cy
Cuts at: 0 171 676 945 946 994 1150 1339 1773
Size: 171 505 269 1 48 156 189 434
Cuts at: 1773 2617 3267 3268 3540
Size: 844 650 1 272
Fragments arranged by size:
844 650 505 434 272 269 189 171
156 48 1 1
HaeII r_GCGC'y
Cuts at: 0 151 417 2200 2361 2378 3484 3540
Size: 151 266 1783 161 17 1106 56
Fragments arranged by size:
1783 1106 266 161 151 56 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1539 1571 3540
Size: 1539 32 1969
Fragments arranged by size:
1969 1539 32
KpnI G_GTAC'C
Cuts at: 0 656 3540
Size: 656 2884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1591 2366 3540
Size: 1591 775 1174
Fragments arranged by size:
1591 1174 775
MscI TGG'CCA
Cuts at: 0 1534 2167 2645 3540
Size: 1534 633 478 895
Fragments arranged by size:
1534 895 633 478
MslI CAynn'nnrTG
Cuts at: 0 311 559 1940 3010 3212 3235 3540
Size: 311 248 1381 1070 202 23 305
Fragments arranged by size:
1381 1070 311 305 248 202 23
MspA1I CmG'CkG
Cuts at: 0 1789 1983 2058 2623 3303 3540
Size: 1789 194 75 565 680 237
Fragments arranged by size:
1789 680 565 237 194 75
NaeI GCC'GGC
Cuts at: 0 51 3540
Size: 51 3489
NcoI C'CATG_G
Cuts at: 0 2858 3540
Size: 2858 682
NgoMIV G'CCGG_C
Cuts at: 0 49 3540
Size: 49 3491
NsiI A_TGCA'T
Cuts at: 0 2150 3527 3540
Size: 2150 1377 13
Fragments arranged by size:
2150 1377 13
NspI r_CATG'y
Cuts at: 0 1184 1256 1412 2531 3525 3540
Size: 1184 72 156 1119 994 15
Fragments arranged by size:
1184 1119 994 156 72 15
PasI CC'CwG_GG
Cuts at: 0 942 1778 2384 3540
Size: 942 836 606 1156
Fragments arranged by size:
1156 942 836 606
PciI A'CATG_T
Cuts at: 0 1408 3540
Size: 1408 2132
PflMI CCAn_nnn'nTGG
Cuts at: 0 381 3540
Size: 381 3159
PfoI T'CCnGG_A
Cuts at: 0 128 398 1761 1897 3059 3148 3540
Size: 128 270 1363 136 1162 89 392
Fragments arranged by size:
1363 1162 392 270 136 128 89
PmlI CAC'GTG
Cuts at: 0 1938 2261 2433 2566 3008 3540
Size: 1938 323 172 133 442 532
Fragments arranged by size:
1938 532 442 323 172 133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 562 594 3540
Size: 562 32 2946
Fragments arranged by size:
2946 562 32
PpuMI rG'GwC_Cy
Cuts at: 0 676 1773 3540
Size: 676 1097 1767
Fragments arranged by size:
1767 1097 676
PshAI GACnn'nnGTC
Cuts at: 0 615 2132 3540
Size: 615 1517 1408
Fragments arranged by size:
1517 1408 615
PspOMI G'GGCC_C
Cuts at: 0 945 994 1150 2616 3267 3540
Size: 945 49 156 1466 651 273
Fragments arranged by size:
1466 945 651 273 156 49
PstI C_TGCA'G
Cuts at: 0 610 635 1788 2766 2920 3540
Size: 610 25 1153 978 154 620
Fragments arranged by size:
1153 978 620 610 154 25
PvuII CAG'CTG
Cuts at: 0 1789 1983 3303 3540
Size: 1789 194 1320 237
Fragments arranged by size:
1789 1320 237 194
RsrII CG'GwC_CG
Cuts at: 0 2732 3540
Size: 2732 808
SacI G_AGCT'C
Cuts at: 0 3148 3540
Size: 3148 392
SacII CC_GC'GG
Cuts at: 0 2624 3540
Size: 2624 916
SanDI GG'GwC_CC
Cuts at: 0 1773 3540
Size: 1773 1767
SapI GCTCTTCn'nnn_
Cuts at: 0 1309 3540
Size: 1309 2231
SfcI C'TryA_G
Cuts at: 0 62 606 631 1784 1949 2762 2916 2955
Size: 62 544 25 1153 165 813 154 39
Cuts at: 2955 3540
Size: 585
Fragments arranged by size:
1153 813 585 544 165 154 62 39
25
SmaI CCC'GGG
Cuts at: 0 1337 1975 2233 3273 3540
Size: 1337 638 258 1040 267
Fragments arranged by size:
1337 1040 638 267 258
SmlI C'TyrA_G
Cuts at: 0 175 1543 3540
Size: 175 1368 1997
Fragments arranged by size:
1997 1368 175
StuI AGG'CCT
Cuts at: 0 268 406 2068 3540
Size: 268 138 1662 1472
Fragments arranged by size:
1662 1472 268 138
StyI C'CwwG_G
Cuts at: 0 182 389 1655 1661 2063 2333 2858 3540
Size: 182 207 1266 6 402 270 525 682
Fragments arranged by size:
1266 682 525 402 270 207 182 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 100 1104 1668 1843 2320 2526 3000 3062
Size: 100 1004 564 175 477 206 474 62
Cuts at: 3062 3540
Size: 478
Fragments arranged by size:
1004 564 478 477 474 206 175 100
62
Tth111I GACn'n_nGTC
Cuts at: 0 222 2226 3540
Size: 222 2004 1314
Fragments arranged by size:
2004 1314 222
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 450 1872 2362 3540
Size: 450 1422 490 1178
Fragments arranged by size:
1422 1178 490 450
XmaI C'CCGG_G
Cuts at: 0 1335 1973 2231 3271 3540
Size: 1335 638 258 1040 269
Fragments arranged by size:
1335 1040 638 269 258
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AloI AlwNI ApaI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KpnI MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuII RsrII SacI SacII SanDI SapI
SfcI SmaI SmlI StuI StyI TaqII Tth111I XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BglII BmgBI BmtI BsiWI
BsmBI BsrGI BssHII BstBI BstZ17I ClaI CspCI DraI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI MfeI MluI NarI NdeI NheI NotI
NruI PacI PmeI PsiI PspXI PsrI PvuI SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI XbaI
XhoI XmnI ZraI