Restriction map for
pF1KB8402
(Linear) (Six-base) MAPSORT of: KIBB8402.seq Check: 6910 from: 1 to: 2346
KIBB8402.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AatII G_ACGT'C
Cuts at: 0 536 2346
Size: 536 1810
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 372 1639 1663 1675 1831 2080 2346
Size: 186 186 1267 24 12 156 249 266
Fragments arranged by size:
1267 266 249 186 186 156 24 12
AfeI AGC'GCT
Cuts at: 0 910 1129 1213 1633 1885 2346
Size: 910 219 84 420 252 461
Fragments arranged by size:
910 461 420 252 219 84
AflIII A'CryG_T
Cuts at: 0 640 2346
Size: 640 1706
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1255 1287 1507 1539 1675 1707 2346
Size: 1255 32 220 32 136 32 639
Fragments arranged by size:
1255 639 220 136 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1160 1404 1419 1617 1839 1923 2088 2346
Size: 1160 244 15 198 222 84 165 258
Fragments arranged by size:
1160 258 244 222 198 165 84 15
ApaI G_GGCC'C
Cuts at: 0 799 1103 2346
Size: 799 304 1243
Fragments arranged by size:
1243 799 304
ApaLI G'TGCA_C
Cuts at: 0 891 1006 1588 1756 2346
Size: 891 115 582 168 590
Fragments arranged by size:
891 590 582 168 115
ApoI r'AATT_y
Cuts at: 0 315 658 2346
Size: 315 343 1688
Fragments arranged by size:
1688 343 315
AvaI C'yCGr_G
Cuts at: 0 412 701 1011 2182 2346
Size: 412 289 310 1171 164
Fragments arranged by size:
1171 412 310 289 164
BamHI G'GATC_C
Cuts at: 0 373 2346
Size: 373 1973
BanI G'GyrC_C
Cuts at: 0 944 1409 2162 2346
Size: 944 465 753 184
Fragments arranged by size:
944 753 465 184
BanII G_rGCy'C
Cuts at: 0 200 421 444 799 1103 1494 2346
Size: 200 221 23 355 304 391 852
Fragments arranged by size:
852 391 355 304 221 200 23
BbvCI CC'TCA_GC
Cuts at: 0 2158 2346
Size: 2158 188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 393 427 1281 1315 2346
Size: 393 34 854 34 1031
Fragments arranged by size:
1031 854 393 34 34
BfrBI ATG'CAT
Cuts at: 0 261 2346
Size: 261 2085
BglI GCCn_nnn'nGGC
Cuts at: 0 15 1646 2084 2187 2346
Size: 15 1631 438 103 159
Fragments arranged by size:
1631 438 159 103 15
BlpI GC'TnA_GC
Cuts at: 0 1462 1825 2074 2308 2346
Size: 1462 363 249 234 38
Fragments arranged by size:
1462 363 249 234 38
Bme1580I G_kGCm'C
Cuts at: 0 799 895 1010 1103 1450 1592 1760 1786
Size: 799 96 115 93 347 142 168 26
Cuts at: 1786 2346
Size: 560
Fragments arranged by size:
799 560 347 168 142 115 96 93
26
BmrI ACTGGGnnnn_n'
Cuts at: 0 461 882 913 2346
Size: 461 421 31 1433
Fragments arranged by size:
1433 461 421 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 716 2346
Size: 716 1630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 981 1196 1495 2346
Size: 981 215 299 851
Fragments arranged by size:
981 851 299 215
Bpu10I CC'TnA_GC
Cuts at: 0 2158 2346
Size: 2158 188
BsaI GGTCTCn'nnnn_
Cuts at: 0 1260 1764 2346
Size: 1260 504 582
Fragments arranged by size:
1260 582 504
BsaAI yAC'GTr
Cuts at: 0 643 2346
Size: 643 1703
BsaHI Gr'CG_yC
Cuts at: 0 434 533 2346
Size: 434 99 1813
Fragments arranged by size:
1813 434 99
BsaWI w'CCGG_w
Cuts at: 0 635 719 803 1358 1869 2346
Size: 635 84 84 555 511 477
Fragments arranged by size:
635 555 511 477 84 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1255 1285 1507 1537 1675 1705 2346
Size: 1255 30 222 30 138 30 641
Fragments arranged by size:
1255 641 222 138 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 237 434 2346
Size: 237 197 1912
Fragments arranged by size:
1912 237 197
BseYI C'CCAG_C
Cuts at: 0 554 850 2346
Size: 554 296 1496
Fragments arranged by size:
1496 554 296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1405 1825 1909 2074 2346
Size: 1405 420 84 165 272
Fragments arranged by size:
1405 420 272 165 84
BsiHKAI G_wGCw'C
Cuts at: 0 553 895 1010 1328 1580 1592 1748 1760
Size: 553 342 115 318 252 12 156 12
Cuts at: 1760 1997 2249 2346
Size: 237 252 97
Fragments arranged by size:
553 342 318 252 252 237 156 115
97 12 12
BsmBI CGTCTCn'nnnn_
Cuts at: 0 141 670 2346
Size: 141 529 1676
Fragments arranged by size:
1676 529 141
Bsp1286I G_dGCh'C
Cuts at: 0 200 421 444 553 799 895 1010 1103
Size: 200 221 23 109 246 96 115 93
Cuts at: 1103 1328 1450 1494 1580 1592 1748 1760 1786
Size: 225 122 44 86 12 156 12 26
Cuts at: 1786 1997 2249 2346
Size: 211 252 97
Fragments arranged by size:
252 246 225 221 211 200 156 122
115 109 97 96 93 86 44 26
23 12 12
BspEI T'CCGG_A
Cuts at: 0 803 1869 2346
Size: 803 1066 477
Fragments arranged by size:
1066 803 477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1227 2346
Size: 1227 1119
BsrDI GCAATG_nn'
Cuts at: 0 1200 2330 2346
Size: 1200 1130 16
Fragments arranged by size:
1200 1130 16
BsrFI r'CCGG_y
Cuts at: 0 941 966 1496 2346
Size: 941 25 530 850
Fragments arranged by size:
941 850 530 25
BssSI C'ACGA_G
Cuts at: 0 835 1695 2346
Size: 835 860 651
Fragments arranged by size:
860 835 651
BstAPI GCAn_nnn'nTGC
Cuts at: 0 899 2346
Size: 899 1447
BstXI CCAn_nnnn'nTGG
Cuts at: 0 510 2346
Size: 510 1836
BstYI r'GATC_y
Cuts at: 0 72 348 373 513 2346
Size: 72 276 25 140 1833
Fragments arranged by size:
1833 276 140 72 25
BtgI C'CryG_G
Cuts at: 0 688 987 2346
Size: 688 299 1359
Fragments arranged by size:
1359 688 299
BtsI GCAGTG_nn'
Cuts at: 0 1008 1315 2112 2346
Size: 1008 307 797 234
Fragments arranged by size:
1008 797 307 234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 688 736 1207 1459 1627 1795 2044
Size: 19 669 48 471 252 168 168 249
Cuts at: 2044 2346
Size: 302
Fragments arranged by size:
669 471 302 252 249 168 168 48
19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 372 981 1196 1495 1639 1663 1675
Size: 186 186 609 215 299 144 24 12
Cuts at: 1675 1831 2080 2346
Size: 156 249 266
Fragments arranged by size:
609 299 266 249 215 186 186 156
144 24 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 398 1039 1234 2346
Size: 398 641 195 1112
Fragments arranged by size:
1112 641 398 195
EcoO109I rG'GnC_Cy
Cuts at: 0 499 647 795 796 992 1099 1100 1604
Size: 499 148 148 1 196 107 1 504
Cuts at: 1604 1856 2085 2189 2346
Size: 252 229 104 157
Fragments arranged by size:
504 499 252 229 196 157 148 148
107 104 1 1
EcoRI G'AATT_C
Cuts at: 0 315 2346
Size: 315 2031
FseI GG_CCGG'CC
Cuts at: 0 970 2346
Size: 970 1376
FspI TGC'GCA
Cuts at: 0 1199 2346
Size: 1199 1147
FspAI rTGC'GCAy
Cuts at: 0 1199 2346
Size: 1199 1147
HaeII r_GCGC'y
Cuts at: 0 912 1081 1131 1145 1215 1635 1839 1887
Size: 912 169 50 14 70 420 204 48
Cuts at: 1887 2346
Size: 459
Fragments arranged by size:
912 459 420 204 169 70 50 48
14
HindIII A'AGCT_T
Cuts at: 0 2236 2346
Size: 2236 110
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 855 2346
Size: 855 1491
MspA1I CmG'CkG
Cuts at: 0 288 989 1343 1401 1416 1595 1763 1821
Size: 288 701 354 58 15 179 168 58
Cuts at: 1821 1920 1931 2070 2346
Size: 99 11 139 276
Fragments arranged by size:
701 354 288 276 179 168 139 99
58 58 15 11
NaeI GCC'GGC
Cuts at: 0 968 2346
Size: 968 1378
NgoMIV G'CCGG_C
Cuts at: 0 966 2346
Size: 966 1380
NsiI A_TGCA'T
Cuts at: 0 263 2346
Size: 263 2083
NspI r_CATG'y
Cuts at: 0 109 1485 2157 2346
Size: 109 1376 672 189
Fragments arranged by size:
1376 672 189 109
PflMI CCAn_nnn'nTGG
Cuts at: 0 1456 2346
Size: 1456 890
PfoI T'CCnGG_A
Cuts at: 0 175 2346
Size: 175 2171
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1255 1287 1507 1539 1675 1707 2346
Size: 1255 32 220 32 136 32 639
Fragments arranged by size:
1255 639 220 136 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 499 2346
Size: 499 1847
PshAI GACnn'nnGTC
Cuts at: 0 133 2346
Size: 133 2213
PspOMI G'GGCC_C
Cuts at: 0 795 1099 2346
Size: 795 304 1247
Fragments arranged by size:
1247 795 304
PstI C_TGCA'G
Cuts at: 0 1085 1165 1238 1282 2346
Size: 1085 80 73 44 1064
Fragments arranged by size:
1085 1064 80 73 44
PvuII CAG'CTG
Cuts at: 0 288 1401 1821 1920 2070 2346
Size: 288 1113 420 99 150 276
Fragments arranged by size:
1113 420 288 276 150 99
SacII CC_GC'GG
Cuts at: 0 990 2346
Size: 990 1356
SbfI CC_TGCA'GG
Cuts at: 0 1165 1282 2346
Size: 1165 117 1064
Fragments arranged by size:
1165 1064 117
SfcI C'TryA_G
Cuts at: 0 1081 1161 1234 1278 1530 2346
Size: 1081 80 73 44 252 816
Fragments arranged by size:
1081 816 252 80 73 44
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2187 2346
Size: 2187 159
SgrAI Cr'CCGG_yG
Cuts at: 0 941 2346
Size: 941 1405
SphI G_CATG'C
Cuts at: 0 1485 2346
Size: 1485 861
StuI AGG'CCT
Cuts at: 0 393 2106 2346
Size: 393 1713 240
Fragments arranged by size:
1713 393 240
StyI C'CwwG_G
Cuts at: 0 399 562 2346
Size: 399 163 1784
Fragments arranged by size:
1784 399 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 568 1570 2346
Size: 568 1002 776
Fragments arranged by size:
1002 776 568
TatI w'GTAC_w
Cuts at: 0 2256 2346
Size: 2256 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2251 2283 2346
Size: 2251 32 63
Fragments arranged by size:
2251 63 32
XbaI T'CTAG_A
Cuts at: 0 75 141 2346
Size: 75 66 2205
Fragments arranged by size:
2205 75 66
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 367 2346
Size: 367 1979
XmnI GAAnn'nnTTC
Cuts at: 0 1354 2218 2346
Size: 1354 864 128
Fragments arranged by size:
1354 864 128
ZraI GAC'GTC
Cuts at: 0 534 2346
Size: 534 1812
Enzymes that do cut:
AatII AcuI AfeI AflIII AloI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbvCI BcgI BfrBI
BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BssSI BstAPI
BstXI BstYI BtgI BtsI EciI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI FspAI HaeII HindIII MmeI MspA1I
NaeI NgoMIV NsiI NspI PflMI PfoI PpiI PpuMI
PshAI PspOMI PstI PvuII SacII SbfI SfcI SfiI
SgrAI SphI StuI StyI TaqII TatI TstI XbaI
XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BciVI BclI BglII BmgBI BmtI BpuEI BsaBI BsiEI
BsiWI BsmI BspHI BsrBI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EcoICRI EcoRV FalI Hin4I HincII
HpaI KasI KpnI MfeI MluI MscI MslI NarI
NcoI NdeI NheI NotI NruI PacI PasI PciI
PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacI
SalI SanDI SapI ScaI SexAI SfoI SgfI SmaI
SmlI SnaBI SpeI SrfI SspI SwaI Tth111I XhoI
XmaI