(Linear) (Six-base) MAPSORT of: KIBB8400.seq  Check: 8849  from: 1  to: 633



KIBB8400.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     629     633 
   Size:        629       4
 
AlwNI CAG_nnn'CTG

Cuts at:      0     139     633 
   Size:        139     494
 
BanI G'GyrC_C

Cuts at:      0     435     633 
   Size:        435     198
 
BanII G_rGCy'C

Cuts at:      0     110     633 
   Size:        110     523
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     196     633 
   Size:        196     437
 
BclI T'GATC_A

Cuts at:      0     453     633 
   Size:        453     180
 
BglI GCCn_nnn'nGGC

Cuts at:      0     444     633 
   Size:        444     189
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     106     124     633 
   Size:         57      49      18     509

  Fragments arranged by size: 

                 509      57      49      18
 
Bpu10I CC'TnA_GC

Cuts at:      0       5     109     633 
   Size:          5     104     524

  Fragments arranged by size: 

                 524     104       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      42      45     633 
   Size:         42       3     588

  Fragments arranged by size: 

                 588      42       3
 
BseYI C'CCAG_C

Cuts at:      0     311     633 
   Size:        311     322
 
BsiHKAI G_wGCw'C

Cuts at:      0     110     489     633 
   Size:        110     379     144

  Fragments arranged by size: 

                 379     144     110
 
BsmI GAATG_Cn'

Cuts at:      0     576     633 
   Size:        576      57
 
Bsp1286I G_dGCh'C

Cuts at:      0     110     489     633 
   Size:        110     379     144

  Fragments arranged by size: 

                 379     144     110
 
BsrBI CCG'CTC

Cuts at:      0      50      71     633 
   Size:         50      21     562

  Fragments arranged by size: 

                 562      50      21
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     139     633 
   Size:        139     494
 
BtgI C'CryG_G

Cuts at:      0      59     633 
   Size:         59     574
 
EcoICRI GAG'CTC

Cuts at:      0     108     633 
   Size:        108     525
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     106     124     633 
   Size:         57      49      18     509

  Fragments arranged by size: 

                 509      57      49      18
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     181     633 
   Size:        181     452
 
HaeII r_GCGC'y

Cuts at:      0      42     633 
   Size:         42     591
 
HindIII A'AGCT_T

Cuts at:      0     119     633 
   Size:        119     514
 
MspA1I CmG'CkG

Cuts at:      0     102     633 
   Size:        102     531
 
NcoI C'CATG_G

Cuts at:      0      59     633 
   Size:         59     574
 
NspI r_CATG'y

Cuts at:      0     337     425     633 
   Size:        337      88     208

  Fragments arranged by size: 

                 337     208      88
 
PvuII CAG'CTG

Cuts at:      0     102     633 
   Size:        102     531
 
SacI G_AGCT'C

Cuts at:      0     110     633 
   Size:        110     523
 
SfcI C'TryA_G

Cuts at:      0     414     633 
   Size:        414     219
 
SphI G_CATG'C

Cuts at:      0     337     633 
   Size:        337     296
 
SspI AAT'ATT

Cuts at:      0     540     633 
   Size:        540      93
 
StyI C'CwwG_G

Cuts at:      0      59     633 
   Size:         59     574
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      81     633 
   Size:         81     552
 
XmnI GAAnn'nnTTC

Cuts at:      0     164     633 
   Size:        164     469
 

 Enzymes that do cut:

     AlfI    AlwNI     BanI    BanII    BciVI     BclI     BglI     BpmI
   Bpu10I    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BsrBI   BstAPI
     BtgI  EcoICRI  Eco57MI     FalI    HaeII  HindIII   MspA1I     NcoI
     NspI    PvuII     SacI     SfcI     SphI     SspI     StyI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     ZraI